PMID- 10536157
OWN - NLM
STAT- MEDLINE
DCOM- 19991228
LR  - 20190501
IS  - 1362-4962 (Electronic)
IS  - 0305-1048 (Linking)
VI  - 27
IP  - 22
DP  - 1999 Nov 15
TI  - The human REV1 gene codes for a DNA template-dependent dCMP transferase.
PG  - 4468-75
AB  - DNA is frequently damaged by various physical and chemical agents. DNA damage can
      lead to mutations during replication. In the yeast Saccharomyces cerevisiae, the 
      damage-induced mutagenesis pathway requires the Rev1 protein. We have isolated a 
      human cDNA homologous to the yeast REV1 gene. The human REV1 cDNA consists of
      4255 bp and codes for a protein of 1251 amino acid residues with a calculated
      molecular weight of 138 248 Da. The human REV1 gene is localized between 2q11.1
      and 2q11.2. We show that the human REV1 protein is a dCMP transferase that
      specifically inserts a dCMP residue opposite a DNA template G. In addition, the
      human REV1 transferase is able to efficiently and specifically insert a dCMP
      opposite a DNA template apurinic/apyrimidinic (AP) site or a uracil residue.
      These results suggest that the REV1 transferase may play a critical role during
      mutagenic translesion DNA synthesis bypassing a template AP site in human cells. 
      Consistent with its role as a fundamental mutagenic protein, the REV1 gene is
      ubiquitously expressed in various human tissues.
FAU - Lin, W
AU  - Lin W
AD  - Graduate Center for Toxicology, University of Kentucky, Lexington, KY 40536, USA.
FAU - Xin, H
AU  - Xin H
FAU - Zhang, Y
AU  - Zhang Y
FAU - Wu, X
AU  - Wu X
FAU - Yuan, F
AU  - Yuan F
FAU - Wang, Z
AU  - Wang Z
LA  - eng
SI  - GENBANK/AF151538
SI  - GENBANK/AF153594
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - Nucleic Acids Res
JT  - Nucleic acids research
JID - 0411011
RN  - 0 (DNA, Complementary)
RN  - 0 (Fungal Proteins)
RN  - 0 (Saccharomyces cerevisiae Proteins)
RN  - 1032-65-1 (Deoxycytidine Monophosphate)
RN  - EC 2.7.7.- (Nucleotidyltransferases)
RN  - EC 2.7.7.- (REV1 protein, S cerevisiae)
SB  - IM
MH  - Amino Acid Sequence
MH  - Chromosome Mapping
MH  - Chromosomes, Human, Pair 2
MH  - Cloning, Organism
MH  - Conserved Sequence
MH  - DNA, Complementary/analysis
MH  - Deoxycytidine Monophosphate/metabolism
MH  - Fungal Proteins/chemistry/*genetics
MH  - Humans
MH  - Molecular Sequence Data
MH  - *Nucleotidyltransferases
MH  - Saccharomyces cerevisiae
MH  - *Saccharomyces cerevisiae Proteins
MH  - Sequence Homology, Amino Acid
MH  - Templates, Genetic
PMC - PMC148731
EDAT- 1999/10/28 00:00
MHDA- 1999/10/28 00:01
CRDT- 1999/10/28 00:00
PHST- 1999/10/28 00:00 [pubmed]
PHST- 1999/10/28 00:01 [medline]
PHST- 1999/10/28 00:00 [entrez]
AID - gkc648 [pii]
AID - 10.1093/nar/27.22.4468 [doi]
PST - ppublish
SO  - Nucleic Acids Res. 1999 Nov 15;27(22):4468-75. doi: 10.1093/nar/27.22.4468.