PMID- 10536140
OWN - NLM
STAT- MEDLINE
DCOM- 19991228
LR  - 20190501
IS  - 1362-4962 (Electronic)
IS  - 0305-1048 (Linking)
VI  - 27
IP  - 22
DP  - 1999 Nov 15
TI  - Multi-forms of human MTH1 polypeptides produced by alternative translation
      initiation and single nucleotide polymorphism.
PG  - 4335-43
AB  - The human MTH1 gene for 8-oxo-7,8-dihydrodeoxyguanosine triphosphatase, produces 
      seven types (types 1, 2A, 2B, 3A, 3B, 4A and 4B) of mRNAs. The B-type mRNAs with 
      exon 2b-2c segments have three additional in-frame AUGs in their 5' regions. We
      report here that these transcripts produce three forms of MTH1 polypeptides (p22,
      p21 and p18) in in vitro translation reactions. Three polypeptides were also
      detected in extracts of human cells, using western blotting. B-type mRNAs with a 
      polymorphic alteration (GU-->GC) at the beginning of exon 2c that converts an
      in-frame UGA to CGA yielding another in-frame AUG further upstream, produced an
      additional polypeptide (p26) in vitro. Substitution of each AUG abolished the
      production of each corresponding polypeptide. Cell lines from individuals with
      the GC allele contain more B-type mRNAs than do those of GT homozygotes, and the 
      former produce all of four polypeptides but the latter lack p26. Amounts of each 
      polypeptide reflected copy number of the GC allele in each cell line. There is an
      apparent linkage dis-equilibrium between the two polymorphic sites, GT/GC at exon
      2c and Val83/Met83 at codon 83 for p18.
FAU - Oda, H
AU  - Oda H
AD  - Department of Biochemistry, Medical Institute of Bioregulation, Kyushu
      University, Fukuoka, Japan.
FAU - Taketomi, A
AU  - Taketomi A
FAU - Maruyama, R
AU  - Maruyama R
FAU - Itoh, R
AU  - Itoh R
FAU - Nishioka, K
AU  - Nishioka K
FAU - Yakushiji, H
AU  - Yakushiji H
FAU - Suzuki, T
AU  - Suzuki T
FAU - Sekiguchi, M
AU  - Sekiguchi M
FAU - Nakabeppu, Y
AU  - Nakabeppu Y
LA  - eng
SI  - GENBANK/AB025233
SI  - GENBANK/AB025234
SI  - GENBANK/AB025235
SI  - GENBANK/AB025236
SI  - GENBANK/AB025237
SI  - GENBANK/AB025238
SI  - GENBANK/AB025239
SI  - GENBANK/AB025240
SI  - GENBANK/AB025241
SI  - GENBANK/AB025242
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - Nucleic Acids Res
JT  - Nucleic acids research
JID - 0411011
RN  - 0 (Codon, Initiator)
RN  - 0 (RNA, Messenger)
RN  - EC 3.1.3.2 (Phosphoric Monoester Hydrolases)
RN  - EC 3.6.1.55 (8-oxodGTPase)
RN  - EC 6.5.1.- (DNA Repair Enzymes)
SB  - IM
MH  - Base Sequence
MH  - Codon, Initiator
MH  - *DNA Repair Enzymes
MH  - Exons
MH  - Humans
MH  - In Vitro Techniques
MH  - Lymphocytes/*physiology
MH  - Molecular Sequence Data
MH  - Phosphoric Monoester Hydrolases/*genetics/metabolism/physiology
MH  - *Polymorphism, Single Nucleotide
MH  - *Protein Biosynthesis
MH  - RNA, Messenger/metabolism
PMC - PMC148714
EDAT- 1999/10/28 00:00
MHDA- 1999/10/28 00:01
CRDT- 1999/10/28 00:00
PHST- 1999/10/28 00:00 [pubmed]
PHST- 1999/10/28 00:01 [medline]
PHST- 1999/10/28 00:00 [entrez]
AID - gkc655 [pii]
AID - 10.1093/nar/27.22.4335 [doi]
PST - ppublish
SO  - Nucleic Acids Res. 1999 Nov 15;27(22):4335-43. doi: 10.1093/nar/27.22.4335.