PMID- 10536058
OWN - NLM
STAT- MEDLINE
DCOM- 19991130
LR  - 20071114
IS  - 1058-8388 (Print)
IS  - 1058-8388 (Linking)
VI  - 216
IP  - 2
DP  - 1999 Oct
TI  - Murine prenatal expression of cholecystokinin in neural crest, enteric neurons,
      and enteroendocrine cells.
PG  - 190-200
AB  - Cholecystokinin (CCK) is a regulatory peptide that is primarily expressed in two 
      adult cell types: endocrine cells of the intestine and neurons of the central
      nervous system. To determine the ontogeny of CCK expression during intestinal
      organogenesis, we created a mouse strain in which the CCK gene was replaced by a 
      lacZ reporter cassette using homologous recombination in embryonic stem cells.
      Initially, CCK expression in the developing intestine was limited to the
      myenteric plexus of the enteric nervous system. This expression pattern was
      widespread, extending from the proximal stomach into the colon, yet transient,
      being detected soon after gut tube closure [embryonic day 10.5 (E10.5)] through
      E15.5. Since enteric neurons are derived from the neural crest, we examined
      earlier (E8.5-9.5) embryos and concluded that lacZ was expressed in
      subpopulations of neural tube and neural crest cells. Endocrine cell expression
      in the intestinal epithelium occurred later, beginning at E15.5 as enteric
      neuronal expression was dwindling. This expression persisted to yield the adult
      pattern of scattered single endocrine cells in the upper small intestine. The
      data show that CCK is a very early marker of both neuronal and endocrine cell
      lineages in the developing gastrointestinal tract. Furthermore, reverse
      transcriptase polymerase chain reaction (RT-PCR) analysis showed that CCK
      receptor transcripts were detected in embryos as early as E10.5, suggesting that 
      CCK signaling is established early in mouse development. Dev Dyn
      1999;216:190-200.
CI  - Copyright 1999 Wiley-Liss, Inc.
FAU - Lay, J M
AU  - Lay JM
AD  - Graduate Program in Cellular and Molecular Biology, The University of Michigan,
      Ann Arbor 48109-0622, USA.
FAU - Gillespie, P J
AU  - Gillespie PJ
FAU - Samuelson, L C
AU  - Samuelson LC
LA  - eng
GR  - R01DK48815/DK/NIDDK NIH HHS/United States
GR  - R03MH50582/MH/NIMH NIH HHS/United States
GR  - T32GM07315/GM/NIGMS NIH HHS/United States
GR  - etc.
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - Dev Dyn
JT  - Developmental dynamics : an official publication of the American Association of
      Anatomists
JID - 9201927
RN  - 1393-25-5 (Secretin)
RN  - 63231-63-0 (RNA)
RN  - 9011-97-6 (Cholecystokinin)
RN  - EC 3.2.1.23 (beta-Galactosidase)
SB  - IM
MH  - Animals
MH  - Base Sequence
MH  - Brain/embryology/metabolism
MH  - Cholecystokinin/genetics/*metabolism
MH  - Digestive System/embryology/metabolism
MH  - Enteric Nervous System/*embryology/*metabolism
MH  - Enteroendocrine Cells/*metabolism
MH  - Female
MH  - Gene Expression Regulation, Developmental
MH  - Genes, Reporter
MH  - In Situ Hybridization
MH  - Male
MH  - Mice
MH  - Mice, Mutant Strains
MH  - Neural Crest/*embryology/*metabolism
MH  - RNA/metabolism
MH  - Secretin/genetics
MH  - Stem Cells/metabolism
MH  - beta-Galactosidase/metabolism
EDAT- 1999/10/27 00:00
MHDA- 1999/10/27 00:01
CRDT- 1999/10/27 00:00
PHST- 1999/10/27 00:00 [pubmed]
PHST- 1999/10/27 00:01 [medline]
PHST- 1999/10/27 00:00 [entrez]
AID - 10.1002/(SICI)1097-0177(199910)216:2<190::AID-DVDY9>3.0.CO;2-K [pii]
AID - 10.1002/(SICI)1097-0177(199910)216:2<190::AID-DVDY9>3.0.CO;2-K [doi]
PST - ppublish
SO  - Dev Dyn. 1999 Oct;216(2):190-200. doi:
      10.1002/(SICI)1097-0177(199910)216:2<190::AID-DVDY9>3.0.CO;2-K.