PMID- 10536039
OWN - NLM
STAT- MEDLINE
DCOM- 20000119
LR  - 20190513
IS  - 0959-6658 (Print)
IS  - 0959-6658 (Linking)
VI  - 9
IP  - 11
DP  - 1999 Nov
TI  - A structural comparison of lipopolysaccharides from two strains of Helicobacter
      pylori, of which one strain (442) does and the other strain (471) does not
      stimulate pepsinogen secretion.
PG  - 1235-45
AB  - Lipopolysaccharides (LPSs) from strains of Helicobacter pylori (442 and 471),
      which differed in stimulation of pepsinogen secretion, were isolated as
      water-soluble material of high-M(r), and as water-insoluble gels of low-M(r).
      Chemical and spectroscopic analyses of soluble LPS and oligosaccharides liberated
      from the gels led to proposed structures with Lewis (Le) antigen termini
      connected to N -acetyllacto-saminoglycans of alternating 3-linked beta-D-Gal and 
      4-linked beta-D-GlcNAc residues with various laterally attached glycosyl
      substituents. The LPS of H.pylori 442 was similar to previously examined strains 
      (NCTC 11637 and P466) in having partially glycosylated chains with alpha-L-Fuc
      units attached to O-3 of the majority of GlcNAc residues in Le(x)units, and in
      chain termination with Le(x)or Le(y)determinants. In contrast, terminal
      Le(y)units occurred in LPS of H.pylori 471 and glycosaminoglycan chains carried a
      smaller proportion of alpha-L-Fuc units, but at O-6 of a majority of
      nonfucosylated GlcNAc residues, there was a novel type of branching with
      alpha-D-Gal substituents. Evidence for the branched regions was obtained
      from(1)H-NMR spectra and from characterization of oligosaccharides formed by the 
      action of endo-beta-galactosidase. Examination of oligosaccharides liberated from
      water-insoluble LPS gels of H.pylori 442 and 471 provided evidence for similar
      core OS structures to those from other H.pylori strains but interesting
      differences were observed.
FAU - Aspinall, G O
AU  - Aspinall GO
AD  - Department of Chemistry, York University, North York, ON M3J 1P3, Canada.
FAU - Mainkar, A S
AU  - Mainkar AS
FAU - Moran, A P
AU  - Moran AP
LA  - eng
PT  - Comparative Study
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - Glycobiology
JT  - Glycobiology
JID - 9104124
RN  - 0 (Antigens, Bacterial)
RN  - 0 (Lewis Blood-Group System)
RN  - 0 (Lipopolysaccharides)
RN  - 0 (O Antigens)
RN  - 0 (Oligosaccharides)
RN  - 9001-10-9 (Pepsinogen A)
SB  - IM
MH  - Antigens, Bacterial/*chemistry/immunology
MH  - Carbohydrate Conformation
MH  - Carbohydrate Sequence
MH  - Duodenal Ulcer/microbiology
MH  - Glycosylation
MH  - Helicobacter Infections/microbiology
MH  - Helicobacter pylori/*chemistry/immunology/isolation & purification
MH  - Humans
MH  - Lewis Blood-Group System/*chemistry
MH  - Lipopolysaccharides/*chemistry/immunology
MH  - Magnetic Resonance Spectroscopy
MH  - Molecular Sequence Data
MH  - Molecular Weight
MH  - O Antigens/*chemistry/immunology
MH  - Oligosaccharides/analysis
MH  - Pepsinogen A/*metabolism
MH  - Secretory Rate/drug effects
MH  - Solubility
MH  - Species Specificity
MH  - Stimulation, Chemical
EDAT- 1999/10/27 00:00
MHDA- 1999/10/27 00:01
CRDT- 1999/10/27 00:00
PHST- 1999/10/27 00:00 [pubmed]
PHST- 1999/10/27 00:01 [medline]
PHST- 1999/10/27 00:00 [entrez]
AID - cwc119 [pii]
AID - 10.1093/glycob/9.11.1235 [doi]
PST - ppublish
SO  - Glycobiology. 1999 Nov;9(11):1235-45. doi: 10.1093/glycob/9.11.1235.