PMID- 10536037
OWN - NLM
STAT- MEDLINE
DCOM- 20000119
LR  - 20190513
IS  - 0959-6658 (Print)
IS  - 0959-6658 (Linking)
VI  - 9
IP  - 11
DP  - 1999 Nov
TI  - Cloning and expression of a human gene encoding an
      N-acetylgalactosamine-alpha2,6-sialyltransferase (ST6GalNAc I): a candidate for
      synthesis of cancer-associated sialyl-Tn antigens.
PG  - 1213-24
AB  - The sialyl-Tn (sTn) antigen is a well known cancer-associated antigen, the
      expression of which is related to the prognosis of cancer patients. We aimed to
      isolate a human gene encoding an N -acetylgalactosamine
      alpha2,6-sialyltransferase which synthesizes sTn antigen, and to characterize the
      enzyme. Degenerate primers encoding sialyl motifs were used for the polymerase
      chain reaction to amplify complementary DNAs prepared from RNAs of human pyloric 
      mucosae with intestinal metaplasia, which abundantly expressed sTn antigen,
      followed by screening of full-length cDNAs using the amplified DNA fragment as a 
      probe. We isolated two human cDNA clones, long-form (2.46 kb) and short-form
      (2.23 kb) cDNAs. The former encodes an active enzyme with a predicted 600 amino
      acid sequence. The latter, a splice-variant of the long-form, encodes an inactive
      enzyme. HCT15 human colorectal cancer cells stably expressing the long-form cDNA 
      expressed sTn epitopes on O -glycans. The long form cDNA was considered to encode
      a human homologue of chick ST6GalNAc I for the following reasons: (1) the
      putative amino acid sequence showed greater homology to that of chick ST6GalNAc I
      (55%) compared to other sialyltransferases, (2) it encodes the extraordinarily
      long stem region that is a typical feature of chick ST6GalNAc I, and (3) the
      substrate specificity was very similar to that of chick ST6GalNAc I. In situ
      hybridization demonstrated that the localization of transcripts correlated well
      with that of sTn antigen in gastric cancer cells and Goblet cells in intestinal
      metaplastic glands. Thus, we determined that the long-form cDNA of the human
      ST6GalNAc I gene encodes the probable candidate for the human sTn synthase(s).
FAU - Ikehara, Y
AU  - Ikehara Y
AD  - Division of Cell Biology, Institute of Life Science, Soka University, 1-236
      Tangi-cho, Hachioji, Tokyo 192-8577, Japan.
FAU - Kojima, N
AU  - Kojima N
FAU - Kurosawa, N
AU  - Kurosawa N
FAU - Kudo, T
AU  - Kudo T
FAU - Kono, M
AU  - Kono M
FAU - Nishihara, S
AU  - Nishihara S
FAU - Issiki, S
AU  - Issiki S
FAU - Morozumi, K
AU  - Morozumi K
FAU - Itzkowitz, S
AU  - Itzkowitz S
FAU - Tsuda, T
AU  - Tsuda T
FAU - Nishimura, S I
AU  - Nishimura SI
FAU - Tsuji, S
AU  - Tsuji S
FAU - Narimatsu, H
AU  - Narimatsu H
LA  - eng
SI  - GENBANK/Y11339
SI  - GENBANK/Y11340
GR  - R01 CA52491/CA/NCI NIH HHS/United States
PT  - Comparative Study
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - England
TA  - Glycobiology
JT  - Glycobiology
JID - 9104124
RN  - 0 (Antigens, Tumor-Associated, Carbohydrate)
RN  - 0 (DNA, Complementary)
RN  - 0 (Neoplasm Proteins)
RN  - 0 (Recombinant Fusion Proteins)
RN  - 0 (alpha-Fetoproteins)
RN  - 0 (sialosyl-Tn antigen)
RN  - EC 2.4.99.- (Sialyltransferases)
RN  - EC 2.4.99.3 (CMP-N-acetylneuraminate-alpha-N-acetylgalactosaminide
      alpha-2,6-sialyltransferase)
RN  - V956696549 (Acetylglucosamine)
SB  - IM
MH  - Acetylglucosamine/metabolism
MH  - Adenocarcinoma/metabolism/pathology
MH  - Amino Acid Sequence
MH  - Animals
MH  - Antigens, Tumor-Associated, Carbohydrate/*biosynthesis
MH  - Base Sequence
MH  - Carbohydrate Conformation
MH  - Cell Transformation, Neoplastic
MH  - Chick Embryo
MH  - Cloning, Molecular
MH  - Colorectal Neoplasms/metabolism/pathology
MH  - DNA, Complementary/genetics
MH  - Gastric Mucosa/*metabolism/pathology
MH  - *Genes
MH  - Humans
MH  - In Situ Hybridization
MH  - Intestinal Mucosa/*metabolism/pathology
MH  - Metaplasia
MH  - Molecular Sequence Data
MH  - Neoplasm Proteins/biosynthesis/genetics
MH  - Polymerase Chain Reaction
MH  - Precancerous Conditions/*metabolism/pathology
MH  - Pylorus/*metabolism/pathology
MH  - Recombinant Fusion Proteins/metabolism
MH  - Sialyltransferases/biosynthesis/*genetics
MH  - Species Specificity
MH  - Stomach Neoplasms/metabolism/pathology
MH  - Substrate Specificity
MH  - Transfection
MH  - alpha-Fetoproteins/metabolism
EDAT- 1999/10/27 00:00
MHDA- 1999/10/27 00:01
CRDT- 1999/10/27 00:00
PHST- 1999/10/27 00:00 [pubmed]
PHST- 1999/10/27 00:01 [medline]
PHST- 1999/10/27 00:00 [entrez]
AID - cwc120 [pii]
AID - 10.1093/glycob/9.11.1213 [doi]
PST - ppublish
SO  - Glycobiology. 1999 Nov;9(11):1213-24. doi: 10.1093/glycob/9.11.1213.