PMID- 10535943
OWN - NLM
STAT- MEDLINE
DCOM- 19991210
LR  - 20211203
IS  - 0027-8424 (Print)
IS  - 0027-8424 (Linking)
VI  - 96
IP  - 22
DP  - 1999 Oct 26
TI  - Ku is associated with the telomere in mammals.
PG  - 12454-8
AB  - Telomeres are specialized DNA/protein complexes that comprise the ends of
      eukaryotic chromosomes. The highly expressed Ku heterodimer, composed of 70 and
      80 K(d) subunits (Ku70 and Ku80), is the high-affinity DNA binding component of
      the DNA-dependent protein kinase. Ku is critical for nonhomologous DNA
      double-stranded break repair and site-specific recombination of V(D)J gene
      segments. Ku also plays an important role in telomere maintenance in yeast.
      Herein, we report, using an in vivo crosslinking method, that human and hamster
      telomeric DNAs specifically coimmunoprecipitate with human Ku80 after
      crosslinking. Localization of Ku to the telomere does not depend on the
      DNA-dependent protein kinase catalytic component. These findings suggest a direct
      link between Ku and the telomere in mammalian cells.
FAU - Hsu, H L
AU  - Hsu HL
AD  - Life Sciences Division, Los Alamos National Laboratory, Los Alamos, NM 87545,
      USA.
FAU - Gilley, D
AU  - Gilley D
FAU - Blackburn, E H
AU  - Blackburn EH
FAU - Chen, D J
AU  - Chen DJ
LA  - eng
GR  - GM26259/GM/NIGMS NIH HHS/United States
GR  - R37 CA050519/CA/NCI NIH HHS/United States
GR  - R01 GM026259/GM/NIGMS NIH HHS/United States
GR  - CA50519/CA/NCI NIH HHS/United States
GR  - R37 GM026259/GM/NIGMS NIH HHS/United States
GR  - R01 CA050519/CA/NCI NIH HHS/United States
PT  - Journal Article
PT  - Research Support, U.S. Gov't, Non-P.H.S.
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - Proc Natl Acad Sci U S A
JT  - Proceedings of the National Academy of Sciences of the United States of America
JID - 7505876
RN  - 0 (Antigens, Nuclear)
RN  - 0 (DNA-Binding Proteins)
RN  - 0 (Nuclear Proteins)
RN  - 0 (Saccharomyces cerevisiae Proteins)
RN  - 0 (high affinity DNA-binding factor, S cerevisiae)
RN  - EC 2.7.11.1 (DNA-Activated Protein Kinase)
RN  - EC 2.7.11.1 (PRKDC protein, human)
RN  - EC 2.7.11.1 (Protein Serine-Threonine Kinases)
RN  - EC 3.6.4.- (DNA Helicases)
RN  - EC 3.6.4.12 (XRCC5 protein, human)
RN  - EC 3.6.4.12 (Xrcc6 protein, human)
RN  - EC 4.2.99.- (Ku Autoantigen)
SB  - IM
MH  - Animals
MH  - *Antigens, Nuclear
MH  - CHO Cells
MH  - Catalytic Domain
MH  - Cricetinae
MH  - *DNA Helicases
MH  - DNA-Activated Protein Kinase
MH  - DNA-Binding Proteins/*metabolism
MH  - Dimerization
MH  - Humans
MH  - Ku Autoantigen
MH  - Nuclear Proteins/*metabolism
MH  - Protein Serine-Threonine Kinases/metabolism
MH  - *Saccharomyces cerevisiae Proteins
MH  - *Telomere
MH  - Tumor Cells, Cultured
PMC - PMC22947
EDAT- 1999/10/27 00:00
MHDA- 1999/10/27 00:01
CRDT- 1999/10/27 00:00
PHST- 1999/10/27 00:00 [pubmed]
PHST- 1999/10/27 00:01 [medline]
PHST- 1999/10/27 00:00 [entrez]
AID - 10.1073/pnas.96.22.12454 [doi]
PST - ppublish
SO  - Proc Natl Acad Sci U S A. 1999 Oct 26;96(22):12454-8. doi:
      10.1073/pnas.96.22.12454.