PMID- 10535925
OWN - NLM
STAT- MEDLINE
DCOM- 19991210
LR  - 20211203
IS  - 0027-8424 (Print)
IS  - 0027-8424 (Linking)
VI  - 96
IP  - 22
DP  - 1999 Oct 26
TI  - Covalent modification of the homeodomain-interacting protein kinase 2 (HIPK2) by 
      the ubiquitin-like protein SUMO-1.
PG  - 12350-5
AB  - Posttranslational modifications such as ubiquitination and phosphorylation play
      an important role in the regulation of cellular protein function.
      Homeodomain-interacting protein kinase 2 (HIPK2) is a member of the recently
      identified family of nuclear protein kinases that act as corepressors for
      homeodomain transcription factors. Here, we show that HIPK2 is regulated by a
      ubiquitin-like protein, SUMO-1. We demonstrate that HIPK2 localizes to nuclear
      speckles (dots) by means of a speckle-retention signal. This speckle-retention
      signal contains a domain that interacts with a mouse ubiquitin-like protein
      conjugating (E2) enzyme, mUBC9. In cultured cells, HIPK2 is covalently modified
      by SUMO-1, and the SUMO-1 modification of HIPK2 correlates with its localization 
      to nuclear speckles (dots). Thus, our results provide firm evidence that the
      nuclear protein kinase HIPK2 can be covalently modified by SUMO-1, which directs 
      its localization to nuclear speckles (dots).
FAU - Kim, Y H
AU  - Kim YH
AD  - Laboratory of Molecular Cardiology, National Heart, Lung, and Blood Institute,
      National Institutes of Health, Bethesda, MD 20892, USA.
FAU - Choi, C Y
AU  - Choi CY
FAU - Kim, Y
AU  - Kim Y
LA  - eng
PT  - Journal Article
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - Proc Natl Acad Sci U S A
JT  - Proceedings of the National Academy of Sciences of the United States of America
JID - 7505876
RN  - 0 (Carrier Proteins)
RN  - 0 (DNA Primers)
RN  - 0 (SUMO-1 Protein)
RN  - 0 (Ubiquitins)
RN  - EC 2.3.2.23 (Ubiquitin-Conjugating Enzymes)
RN  - EC 2.7.- (Protein Kinases)
RN  - EC 2.7.1.- (HIPK2 protein, human)
RN  - EC 2.7.1.- (Hipk2 protein, mouse)
RN  - EC 2.7.11.1 (Protein Serine-Threonine Kinases)
RN  - EC 6.- (Ligases)
RN  - EC 6.3.2.- (ubiquitin-conjugating enzyme UBC9)
SB  - IM
MH  - Amino Acid Sequence
MH  - Animals
MH  - Base Sequence
MH  - Carrier Proteins/chemistry/*metabolism
MH  - Cell Line
MH  - DNA Primers
MH  - Humans
MH  - Immunohistochemistry
MH  - Ligases/metabolism
MH  - Mice
MH  - Molecular Sequence Data
MH  - Protein Kinases/chemistry/*metabolism
MH  - *Protein Serine-Threonine Kinases
MH  - SUMO-1 Protein
MH  - Sequence Homology, Amino Acid
MH  - *Ubiquitin-Conjugating Enzymes
MH  - Ubiquitins/*metabolism
PMC - PMC22920
EDAT- 1999/10/27 00:00
MHDA- 1999/10/27 00:01
CRDT- 1999/10/27 00:00
PHST- 1999/10/27 00:00 [pubmed]
PHST- 1999/10/27 00:01 [medline]
PHST- 1999/10/27 00:00 [entrez]
AID - 10.1073/pnas.96.22.12350 [doi]
PST - ppublish
SO  - Proc Natl Acad Sci U S A. 1999 Oct 26;96(22):12350-5. doi:
      10.1073/pnas.96.22.12350.