PMID- 10535767
OWN - NLM
STAT- MEDLINE
DCOM- 19991117
LR  - 20190921
IS  - 0742-3098 (Print)
IS  - 0742-3098 (Linking)
VI  - 27
IP  - 3
DP  - 1999 Oct
TI  - CREB phosphorylation and melatonin biosynthesis in the rat pineal gland:
      involvement of cyclic AMP dependent protein kinase type II.
PG  - 170-82
AB  - Phosphorylation of cyclic AMP response element binding protein (CREB) at amino
      acid serine 133 appears as an important link between the norepinephrine
      (NE)-induced activation of second messenger systems and the stimulation of
      melatonin biosynthesis. Here we investigated in the rat pineal gland: 1) the type
      of protein kinase that mediates CREB phosphorylation: and 2) its impact on
      melatonin biosynthesis. Immunochemical or immunocytochemical demonstration of
      serine133-phosphorylated cyclic AMP regulated element binding protein (pCREB) and
      radioimmunological detection of melatonin revealed that only cyclic AMP-dependent
      protein kinase (PKA) inhibitors suppressed NE-induced CREB phosphorylation and
      stimulation of melatonin biosynthesis, whereas inhibitors of cyclic GMP-dependent
      protein kinase (PKG), mitogen-activated protein kinase kinase, protein kinase C, 
      or calcium-calmodulin-dependent protein kinase (CaMK) were ineffective.
      Investigations with cyclic AMP-agonist pairs that selectively activate either PKA
      type I or II link NE-induced CREB phosphorylation and stimulation of melatonin
      biosynthesis to the activation of PKA type II. Our data suggest that PKA type II 
      plays an important role in the transcriptional control of melatonin biosynthesis 
      in the rat pineal organ.
FAU - Maronde, E
AU  - Maronde E
AD  - Dr. Senckenbergische Anatomie, Anatomisches Institut II, J.W. Goethe Universitat,
      Frankfurt, FRG.
FAU - Wicht, H
AU  - Wicht H
FAU - Tasken, K
AU  - Tasken K
FAU - Genieser, H G
AU  - Genieser HG
FAU - Dehghani, F
AU  - Dehghani F
FAU - Olcese, J
AU  - Olcese J
FAU - Korf, H W
AU  - Korf HW
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - J Pineal Res
JT  - Journal of pineal research
JID - 8504412
RN  - 0 (Cyclic AMP Response Element-Binding Protein)
RN  - 0 (Enzyme Inhibitors)
RN  - 0 (Protein Kinase Inhibitors)
RN  - 17885-08-4 (Phosphoserine)
RN  - EC 2.7.11.11 (Cyclic AMP-Dependent Protein Kinase Type II)
RN  - EC 2.7.11.11 (Cyclic AMP-Dependent Protein Kinases)
RN  - JL5DK93RCL (Melatonin)
RN  - X4W3ENH1CV (Norepinephrine)
SB  - IM
MH  - Animals
MH  - Cell Nucleus/enzymology
MH  - Cyclic AMP Response Element-Binding Protein/analysis/*metabolism
MH  - Cyclic AMP-Dependent Protein Kinase Type II
MH  - Cyclic AMP-Dependent Protein Kinases/antagonists & inhibitors/*metabolism
MH  - Enzyme Inhibitors/pharmacology
MH  - Immunohistochemistry
MH  - Male
MH  - Melatonin/*biosynthesis
MH  - Norepinephrine/pharmacology
MH  - Phosphorylation
MH  - Phosphoserine/analysis/metabolism
MH  - Pineal Gland/chemistry/drug effects/*metabolism
MH  - Protein Kinase Inhibitors
MH  - Rats
MH  - Rats, Wistar
EDAT- 1999/10/27 00:00
MHDA- 1999/10/27 00:01
CRDT- 1999/10/27 00:00
PHST- 1999/10/27 00:00 [pubmed]
PHST- 1999/10/27 00:01 [medline]
PHST- 1999/10/27 00:00 [entrez]
AID - 10.1111/j.1600-079x.1999.tb00613.x [doi]
PST - ppublish
SO  - J Pineal Res. 1999 Oct;27(3):170-82. doi: 10.1111/j.1600-079x.1999.tb00613.x.