PMID- 10535749
OWN - NLM
STAT- MEDLINE
DCOM- 19991104
LR  - 20190623
IS  - 0006-2952 (Print)
IS  - 0006-2952 (Linking)
VI  - 58
IP  - 10
DP  - 1999 Nov 15
TI  - Differential phosphorylation of sites in the linker region of P-glycoprotein by
      protein kinase C isozymes alpha, betaI, betaII, gamma, delta, epsilon, eta, and
      zeta.
PG  - 1587-92
AB  - To determine whether individual protein kinase C (PKC) isozymes differentially
      phosphorylate sites in the linker region of human P-glycoprotein (P-gp), we used 
      a synthetic peptide substrate, PG-2, exactly corresponding to amino acid residues
      spanning the region 656-689 of the multidrug resistance gene (MDRI). All tested
      PKC isozymes phosphorylated PG-2. The maximum phosphate incorporation by
      calcium-dependent PKC isozymes alpha, betaI, betaII, and gamma was 3, 2, 2, and 3
      mol phosphate/mol PG-2, respectively. The maximum phosphate incorporation by
      calcium-independent isozymes delta, epsilon, eta, and zeta was 1.5, 0.5, 1.5, and
      1.5 mol phosphate/mol PG-2, respectively. Two-dimensional tryptic phosphopeptide 
      mapping indicated differential phosphorylation of the PKC consensus sites
      Ser-661, Ser-667, and Ser-671 by individual isozymes, which may be functionally
      significant. These data suggest that differential phosphorylation by PKC
      isoenzymes of PKC sites within the P-gp linker region may play a role in
      modulating P-gp activity.
FAU - Sachs, C W
AU  - Sachs CW
AD  - Department of Medicine, Duke University, Durham, NC, USA.
FAU - Chambers, T C
AU  - Chambers TC
FAU - Fine, R L
AU  - Fine RL
LA  - eng
GR  - 5R21 CA 66228-02/CA/NCI NIH HHS/United States
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, Non-P.H.S.
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - England
TA  - Biochem Pharmacol
JT  - Biochemical pharmacology
JID - 0101032
RN  - 0 (ATP Binding Cassette Transporter, Subfamily B, Member 1)
RN  - 0 (Isoenzymes)
RN  - 0 (Peptide Fragments)
RN  - 0 (Phosphopeptides)
RN  - 0 (Recombinant Proteins)
RN  - EC 2.7.11.13 (Protein Kinase C)
SB  - IM
MH  - ATP Binding Cassette Transporter, Subfamily B, Member 1/*metabolism
MH  - Amino Acid Sequence
MH  - Humans
MH  - In Vitro Techniques
MH  - Isoenzymes/*metabolism
MH  - Molecular Sequence Data
MH  - Peptide Fragments/metabolism
MH  - Peptide Mapping
MH  - Phosphopeptides/analysis
MH  - Phosphorylation
MH  - Protein Kinase C/*metabolism
MH  - Recombinant Proteins/metabolism
EDAT- 1999/10/27 00:00
MHDA- 1999/10/27 00:01
CRDT- 1999/10/27 00:00
PHST- 1999/10/27 00:00 [pubmed]
PHST- 1999/10/27 00:01 [medline]
PHST- 1999/10/27 00:00 [entrez]
AID - S0006295299002403 [pii]
AID - 10.1016/s0006-2952(99)00240-3 [doi]
PST - ppublish
SO  - Biochem Pharmacol. 1999 Nov 15;58(10):1587-92. doi:
      10.1016/s0006-2952(99)00240-3.