PMID- 10535737
OWN - NLM
STAT- MEDLINE
DCOM- 19991110
LR  - 20190705
IS  - 0092-8674 (Print)
IS  - 0092-8674 (Linking)
VI  - 99
IP  - 2
DP  - 1999 Oct 15
TI  - A novel clathrin adaptor complex mediates basolateral targeting in polarized
      epithelial cells.
PG  - 189-98
AB  - Although polarized epithelial cells are well known to maintain distinct apical
      and basolateral plasma membrane domains, the mechanisms responsible for targeting
      membrane proteins to the apical or basolateral surfaces have remained elusive. We
      have identified a novel form of the AP-1 clathrin adaptor complex that contains
      as one of its subunits mu1B, an epithelial cell-specific homolog of the
      ubiquitously expressed mu1A. LLC-PK1 kidney epithelial cells do not express mu1B 
      and missort many basolateral proteins to the apical surface. Stable expression of
      mu1B selectively restored basolateral targeting, improved the overall
      organization of LLC-PK1 monolayers, and had no effect on apical targeting. We
      conclude that basolateral sorting is mediated by an epithelial cell-specific
      version of the AP-1 complex containing mu1B.
FAU - Folsch, H
AU  - Folsch H
AD  - Department of Cell Biology, and Ludwig Institute for Cancer Research, Yale
      University School of Medicine, New Haven, Connecticut 06520-8002, USA.
FAU - Ohno, H
AU  - Ohno H
FAU - Bonifacino, J S
AU  - Bonifacino JS
FAU - Mellman, I
AU  - Mellman I
LA  - eng
GR  - GM-29765/GM/NIGMS NIH HHS/United States
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - Cell
JT  - Cell
JID - 0413066
RN  - 0 (AP1M1 protein, human)
RN  - 0 (AP1M2 protein, human)
RN  - 0 (Adaptor Protein Complex 1)
RN  - 0 (Adaptor Protein Complex alpha Subunits)
RN  - 0 (Adaptor Protein Complex mu Subunits)
RN  - 0 (Adaptor Proteins, Vesicular Transport)
RN  - 0 (Clathrin)
RN  - 0 (Macromolecular Substances)
RN  - 0 (Membrane Proteins)
RN  - 0 (Receptors, Transferrin)
RN  - 0 (Recombinant Proteins)
RN  - 0 (adaptor protein complex 1, mu 1 subunit)
RN  - 0 (adaptor protein complex 1, mu 2 subunit)
SB  - IM
CIN - Cell. 1999 Oct 15;99(2):121-2. PMID: 10535730
MH  - *Adaptor Protein Complex 1
MH  - Adaptor Protein Complex alpha Subunits
MH  - *Adaptor Protein Complex mu Subunits
MH  - Adaptor Proteins, Vesicular Transport
MH  - Animals
MH  - Cell Line
MH  - Cell Membrane/*physiology
MH  - Cell Polarity/*physiology
MH  - Clathrin/*metabolism
MH  - Coated Pits, Cell-Membrane/*physiology
MH  - Dogs
MH  - Epithelial Cells/*physiology
MH  - Humans
MH  - Kidney
MH  - LLC-PK1 Cells/*physiology
MH  - Macromolecular Substances
MH  - Membrane Proteins/*metabolism
MH  - Receptors, Transferrin/genetics/physiology
MH  - Recombinant Proteins/metabolism
MH  - Swine
MH  - Transfection
EDAT- 1999/10/27 00:00
MHDA- 1999/10/27 00:01
CRDT- 1999/10/27 00:00
PHST- 1999/10/27 00:00 [pubmed]
PHST- 1999/10/27 00:01 [medline]
PHST- 1999/10/27 00:00 [entrez]
AID - S0092-8674(00)81650-5 [pii]
AID - 10.1016/s0092-8674(00)81650-5 [doi]
PST - ppublish
SO  - Cell. 1999 Oct 15;99(2):189-98. doi: 10.1016/s0092-8674(00)81650-5.