PMID- 10535736
OWN - NLM
STAT- MEDLINE
DCOM- 19991110
LR  - 20190705
IS  - 0092-8674 (Print)
IS  - 0092-8674 (Linking)
VI  - 99
IP  - 2
DP  - 1999 Oct 15
TI  - Essential role of phosphoinositide metabolism in synaptic vesicle recycling.
PG  - 179-88
AB  - Growing evidence suggests that phosphoinositides play an important role in
      membrane traffic. A polyphosphoinositide phosphatase, synaptojanin 1, was
      identified as a major presynaptic protein associated with endocytic coated
      intermediates. We report here that synaptojanin 1-deficient mice exhibit
      neurological defects and die shortly after birth. In neurons of mutant animals,
      PI(4,5)P2 levels are increased, and clathrin-coated vesicles accumulate in the
      cytomatrix-rich area that surrounds the synaptic vesicle cluster in nerve
      endings. In cell-free assays, reduced phosphoinositide phosphatase activity
      correlated with increased association of clathrin coats with liposomes.
      Intracellular recording in hippocampal slices revealed enhanced synaptic
      depression during prolonged high-frequency stimulation followed by delayed
      recovery. These results provide genetic evidence for a crucial role of
      phosphoinositide metabolism in synaptic vesicle recycling.
FAU - Cremona, O
AU  - Cremona O
AD  - Howard Hughes Medical Institute, Yale University School of Medicine, New Haven,
      Connecticut 06510, USA.
FAU - Di Paolo, G
AU  - Di Paolo G
FAU - Wenk, M R
AU  - Wenk MR
FAU - Luthi, A
AU  - Luthi A
FAU - Kim, W T
AU  - Kim WT
FAU - Takei, K
AU  - Takei K
FAU - Daniell, L
AU  - Daniell L
FAU - Nemoto, Y
AU  - Nemoto Y
FAU - Shears, S B
AU  - Shears SB
FAU - Flavell, R A
AU  - Flavell RA
FAU - McCormick, D A
AU  - McCormick DA
FAU - De Camilli, P
AU  - De Camilli P
LA  - eng
GR  - D.111/Telethon/Italy
GR  - NS26143/NS/NINDS NIH HHS/United States
GR  - NS46128/NS/NINDS NIH HHS/United States
GR  - NS36251/NS/NINDS NIH HHS/United States
GR  - D.061/Telethon/Italy
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - Cell
JT  - Cell
JID - 0413066
RN  - 0 (Enzyme Inhibitors)
RN  - 0 (Nerve Tissue Proteins)
RN  - 0 (Phosphatidylinositols)
RN  - EC 3.1.3.- (synaptojanin)
RN  - EC 3.1.3.2 (Phosphoric Monoester Hydrolases)
SB  - IM
MH  - Animals
MH  - Cell-Free System
MH  - Cerebral Cortex/metabolism
MH  - Coated Pits, Cell-Membrane/metabolism
MH  - Endocytosis
MH  - Enzyme Inhibitors/metabolism
MH  - Exons
MH  - Hippocampus/*physiology
MH  - In Vitro Techniques
MH  - Membrane Potentials
MH  - Mice
MH  - Mice, Knockout
MH  - Microscopy, Electron
MH  - Nerve Endings/metabolism
MH  - Nerve Tissue Proteins/deficiency/genetics/*metabolism
MH  - Neurons/metabolism/*physiology/ultrastructure
MH  - Phosphatidylinositols/*metabolism
MH  - Phosphoric Monoester Hydrolases/deficiency/genetics/*metabolism
MH  - Synaptic Vesicles/*metabolism/ultrastructure
EDAT- 1999/10/27 00:00
MHDA- 1999/10/27 00:01
CRDT- 1999/10/27 00:00
PHST- 1999/10/27 00:00 [pubmed]
PHST- 1999/10/27 00:01 [medline]
PHST- 1999/10/27 00:00 [entrez]
AID - S0092-8674(00)81649-9 [pii]
AID - 10.1016/s0092-8674(00)81649-9 [doi]
PST - ppublish
SO  - Cell. 1999 Oct 15;99(2):179-88. doi: 10.1016/s0092-8674(00)81649-9.