PMID- 10533284
OWN - NLM
STAT- MEDLINE
DCOM- 19991116
LR  - 20081121
IS  - 1357-2725 (Print)
IS  - 1357-2725 (Linking)
VI  - 31
IP  - 9
DP  - 1999 Sep
TI  - AML1, the target of chromosomal rearrangements in human leukemia, regulates the
      expression of human complement receptor type 1 (CR1) gene.
PG  - 933-40
AB  - The human CR1 gene is expressed specifically in hematopoietic cells. It is
      suggested that some cell-type specific factors which involve in gene-specific
      activation or repression exist in cells according to the result that the gene
      expression varies differently depend on differentiation stage. Here, we
      demonstrate that the integrity of a polyomavirus enhancer core sequence,
      5'-TGTGGT-3', is critical to the human CR1 promoter activity. AML1 is a
      site-specific DNA-binding protein that recognizes the enhancer core motif TGTGGT.
      We show that the AML1 binds specifically to this site and activates the human CR1
      promoter. Furthermore, we demonstrate that the Ets binding site (GGAA) located 2 
      bp upstream of the AML1 site is also involved in the regulation of the human CR1 
      promoter activity. Point mutations of either the AML1 or the Ets binding site
      that abolish the binding of the respective factors result in significant
      decreases of the human CR1 promoter activity. These results suggest that AML1 and
      Ets proteins direct the expression of the human CR1 promoter.
FAU - Kim, J H
AU  - Kim JH
AD  - School of Life Sciences, Chungbuk National University, Cheongju, South Korea.
FAU - Lee, S
AU  - Lee S
FAU - Rho, J K
AU  - Rho JK
FAU - Choe, S Y
AU  - Choe SY
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Netherlands
TA  - Int J Biochem Cell Biol
JT  - The international journal of biochemistry & cell biology
JID - 9508482
RN  - 0 (Core Binding Factor Alpha 2 Subunit)
RN  - 0 (DNA-Binding Proteins)
RN  - 0 (Proto-Oncogene Proteins)
RN  - 0 (RUNX1 protein, human)
RN  - 0 (Receptors, Complement)
RN  - 0 (Transcription Factors)
RN  - 9007-49-2 (DNA)
SB  - IM
MH  - Base Sequence
MH  - Cell Line
MH  - Core Binding Factor Alpha 2 Subunit
MH  - DNA/*metabolism
MH  - *DNA-Binding Proteins
MH  - Electroporation
MH  - *Gene Rearrangement
MH  - Humans
MH  - Leukemia/*genetics
MH  - Molecular Sequence Data
MH  - Promoter Regions, Genetic
MH  - *Proto-Oncogene Proteins
MH  - Receptors, Complement/*genetics
MH  - Transcription Factors/*genetics/metabolism
MH  - Tumor Cells, Cultured
EDAT- 1999/10/26 00:00
MHDA- 1999/10/26 00:01
CRDT- 1999/10/26 00:00
PHST- 1999/10/26 00:00 [pubmed]
PHST- 1999/10/26 00:01 [medline]
PHST- 1999/10/26 00:00 [entrez]
AID - S1357272599000485 [pii]
PST - ppublish
SO  - Int J Biochem Cell Biol. 1999 Sep;31(9):933-40.