PMID- 10532805
OWN - NLM
STAT- MEDLINE
DCOM- 19991018
LR  - 20071114
IS  - 1044-7431 (Print)
IS  - 1044-7431 (Linking)
VI  - 14
IP  - 2
DP  - 1999 Aug
TI  - Molecular cloning, tissue-specific expression, and chromosomal localization of a 
      novel nerve growth factor-regulated G-protein- coupled receptor, nrg-1.
PG  - 141-52
AB  - A novel and differentially expressed gene, named nrg-1, was identified by EST
      expression profiling and subsequently isolated as a 2.2-kb full-length clone from
      a rat PC12 cell cDNA library. Sequence analysis reveals that nrg-1 encodes a
      putative seven transmembrane spanning domain protein with structural features
      characteristic of receptors belonging to the G-protein-coupled receptor gene
      superfamily. The 400-amino-acid protein encoded by nrg-1 exhibits a high degree
      of sequence identity (40-44%) to the Edg receptor family; members include Edg-1, 
      Edg-2, Edg-3, Edg-4, and H218. Both Northern analysis andEST expression profiling
      revealed that whole-tissue distribution of nrg-1 mRNA is restricted, found almost
      exclusively in brain. Transcripts of nrg-1 could be ubiquitously detected in
      different regions, with very prominent expression in lower brain regions such as 
      the midbrain, pons,medulla, and spinal cord. In PC12 cells, nerve growth factor
      induces neuronal differentiation and repressed expression of nrg-1. Two other
      agents that differentiate PC12 cells, fibroblast growth factor and dibdutyryl
      cAMP, down-regulated nrg-1 mRNA levels. Epidermal growth factor, and agent that
      does not induce differentiation, did not repress nrg-1 mRNA levels. In a PC12
      cell mutant that is deficient in protein kinase A activity (AB.11), all three
      differentiating agents were unable to down-regulate nrg-1 mRNA. Hence, protein
      kinase A appears to be an obligatory cellular component in nrg-1 mRNA regulation.
      Chromosomal mapping employing a rat somatic cell readiation hybrid panel
      demonstrated that nrg-1 is linked to marker D8Rat54 and tightly associated with
      H218 on chromosome 8.
FAU - Glickman, M
AU  - Glickman M
AD  - Department of Molecular Biology, The Institute of Genomic Research, 9712 Medical 
      Center Drive, Rockville, MD 20850, USA.
FAU - Malek, R L
AU  - Malek RL
FAU - Kwitek-Black, A E
AU  - Kwitek-Black AE
FAU - Jacob, H J
AU  - Jacob HJ
FAU - Lee, N H
AU  - Lee NH
LA  - eng
SI  - GENBANK/AF115249
GR  - HL59781/HL/NHLBI NIH HHS/United States
GR  - NS35231/NS/NINDS NIH HHS/United States
PT  - Journal Article
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - Mol Cell Neurosci
JT  - Molecular and cellular neurosciences
JID - 9100095
RN  - 0 (Glycoproteins)
RN  - 0 (Immediate-Early Proteins)
RN  - 0 (Nerve Growth Factors)
RN  - 0 (Neuregulins)
RN  - 0 (RNA, Messenger)
RN  - 0 (Receptors, Cell Surface)
RN  - 0 (Receptors, G-Protein-Coupled)
RN  - 0 (Receptors, Lysophospholipid)
RN  - 62229-50-9 (Epidermal Growth Factor)
RN  - EC 3.6.1.- (GTP-Binding Proteins)
SB  - IM
MH  - Amino Acid Sequence
MH  - Animals
MH  - Base Sequence
MH  - Brain/*metabolism
MH  - *Chromosome Mapping
MH  - Cloning, Molecular
MH  - Epidermal Growth Factor/pharmacology
MH  - Expressed Sequence Tags
MH  - Female
MH  - GTP-Binding Proteins/metabolism
MH  - *Gene Expression Regulation
MH  - Glycoproteins/chemistry/*genetics
MH  - Humans
MH  - Immediate-Early Proteins/chemistry
MH  - Male
MH  - Molecular Sequence Data
MH  - Nerve Growth Factors/genetics
MH  - Neuregulins
MH  - Organ Specificity
MH  - PC12 Cells
MH  - RNA, Messenger/genetics
MH  - Rats
MH  - Receptors, Cell Surface/chemistry/*genetics
MH  - *Receptors, G-Protein-Coupled
MH  - Receptors, Lysophospholipid
MH  - Sequence Alignment
MH  - Sequence Homology, Amino Acid
MH  - Spinal Cord/*metabolism
MH  - *Transcription, Genetic
EDAT- 1999/10/26 00:00
MHDA- 1999/10/26 00:01
CRDT- 1999/10/26 00:00
PHST- 1999/10/26 00:00 [pubmed]
PHST- 1999/10/26 00:01 [medline]
PHST- 1999/10/26 00:00 [entrez]
AID - S1044-7431(99)90776-5 [pii]
AID - 10.1006/mcne.1999.0776 [doi]
PST - ppublish
SO  - Mol Cell Neurosci. 1999 Aug;14(2):141-52. doi: 10.1006/mcne.1999.0776.