PMID- 10531436
OWN - NLM
STAT- MEDLINE
DCOM- 19991119
LR  - 20191023
IS  - 1529-2401 (Electronic)
IS  - 0270-6474 (Linking)
VI  - 19
IP  - 21
DP  - 1999 Nov 1
TI  - Targeted deletion of a cyclic nucleotide-gated channel subunit (OCNC1):
      biochemical and morphological consequences in adult mice.
PG  - 9313-21
AB  - The olfactory cyclic nucleotide-gated channel subunit 1 (OCNC1) is required for
      signal transduction in olfactory receptor cells. To further investigate the role 
      of this channel in the olfactory system, the biochemical and morphological
      consequences of targeted disruption of OCNC1 were investigated in adult mice.
      Null as compared to wild-type mice had smaller olfactory bulbs, suggesting
      compromised development of the central target of the receptor cells. Ectopic
      olfactory marker protein (OMP)-stained fibers localized to the external plexiform
      layer reflected the relative immaturity of the olfactory bulb in the null mice.
      The olfactory epithelium of the knock-out mouse was thinner and showed lower
      expression of olfactory marker protein and growth-associated protein 43,
      indicating decreases in both generation and maturation of receptor cells.
      Tyrosine hydroxylase (TH) expression in the olfactory bulb, examined as a
      reflection of afferent activity, was reduced in the majority of periglomerular
      neurons but retained in atypical or "necklace" glomeruli localized to posterior
      aspects of the olfactory bulb. Double label studies demonstrated that the
      remaining TH-immunostained neurons received their innervation from a subset of
      receptor cells previously shown to express a phosphodiesterase that differs from 
      that found in most receptor cells. These data indicate that expression of OCNC1
      is required for normal development of the olfactory epithelium and olfactory
      bulb. The robust expression of TH in some periglomerular cells in the OCNC1-null 
      mice suggests that receptor cells innervating these glomeruli may use an
      alternate signal transduction pathway.
FAU - Baker, H
AU  - Baker H
AD  - Cornell University Medical College, The Burke Medical Research Institute, White
      Plains, New York 10605, USA. habaker@med.cornell.edu
FAU - Cummings, D M
AU  - Cummings DM
FAU - Munger, S D
AU  - Munger SD
FAU - Margolis, J W
AU  - Margolis JW
FAU - Franzen, L
AU  - Franzen L
FAU - Reed, R R
AU  - Reed RR
FAU - Margolis, F L
AU  - Margolis FL
LA  - eng
GR  - AG09686/AG/NIA NIH HHS/United States
GR  - DC03112/DC/NIDCD NIH HHS/United States
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - J Neurosci
JT  - The Journal of neuroscience : the official journal of the Society for
      Neuroscience
JID - 8102140
RN  - 0 (Cyclic Nucleotide-Gated Cation Channels)
RN  - 0 (GAP-43 Protein)
RN  - 0 (Ion Channels)
RN  - 0 (Nerve Tissue Proteins)
RN  - 0 (Olfactory Marker Protein)
RN  - 0 (Omp protein, mouse)
SB  - IM
MH  - Animals
MH  - Cyclic Nucleotide-Gated Cation Channels
MH  - Female
MH  - GAP-43 Protein/genetics
MH  - Genotype
MH  - Ion Channels/deficiency/genetics/*physiology
MH  - Male
MH  - Mice
MH  - Mice, Knockout
MH  - Nerve Fibers/physiology/ultrastructure
MH  - Nerve Tissue Proteins/analysis/genetics
MH  - Olfactory Bulb/abnormalities/*physiology
MH  - Olfactory Marker Protein
MH  - Olfactory Mucosa/abnormalities/cytology/pathology/physiology
MH  - Olfactory Receptor Neurons/cytology/pathology/*physiology
PMC - PMC6782908
EDAT- 1999/10/26 00:00
MHDA- 1999/10/26 00:01
CRDT- 1999/10/26 00:00
PHST- 1999/10/26 00:00 [pubmed]
PHST- 1999/10/26 00:01 [medline]
PHST- 1999/10/26 00:00 [entrez]
PST - ppublish
SO  - J Neurosci. 1999 Nov 1;19(21):9313-21.