PMID- 10531362
OWN - NLM
STAT- MEDLINE
DCOM- 19991216
LR  - 20190508
IS  - 0021-9258 (Print)
IS  - 0021-9258 (Linking)
VI  - 274
IP  - 44
DP  - 1999 Oct 29
TI  - Interaction and functional cooperation of PEBP2/CBF with Smads. Synergistic
      induction of the immunoglobulin germline Calpha promoter.
PG  - 31577-82
AB  - Smads are signal transducers for members of the transforming growth factor-beta
      (TGF-beta) superfamily. Upon ligand stimulation, receptor-regulated Smads
      (R-Smads) are phosphorylated by serine/threonine kinase receptors, form complexes
      with common-partner Smad, and translocate into the nucleus, where they regulate
      the transcription of target genes together with other transcription factors.
      Polyomavirus enhancer binding protein 2/core binding factor (PEBP2/CBF) is a
      transcription factor complex composed of alpha and beta subunits. The alpha
      subunits of PEBP2/CBF, which contain the highly conserved Runt domain, play
      essential roles in hematopoiesis and osteogenesis. Here we show that three
      mammalian alpha subunits of PEBP2/CBF form complexes with R-Smads that act in
      TGF-beta/activin pathways as well as those acting in bone morphogenetic protein
      (BMP) pathways. Among them, PEBP2alphaC/CBFA3/AML2 forms a complex with Smad3 and
      stimulates transcription of the germline Ig Calpha promoter in a cooperative
      manner, for which binding of both factors to their specific binding sites is
      essential. PEBP2 may thus be a nuclear target of TGF-beta/BMP signaling.
FAU - Hanai, J
AU  - Hanai J
AD  - Department of Biochemistry, The Cancer Institute of the Japanese Foundation for
      Cancer Research, 1-37-1 Kami-ikebukuro, Toshima-ku, Tokyo 70-8455, Japan.
FAU - Chen, L F
AU  - Chen LF
FAU - Kanno, T
AU  - Kanno T
FAU - Ohtani-Fujita, N
AU  - Ohtani-Fujita N
FAU - Kim, W Y
AU  - Kim WY
FAU - Guo, W H
AU  - Guo WH
FAU - Imamura, T
AU  - Imamura T
FAU - Ishidou, Y
AU  - Ishidou Y
FAU - Fukuchi, M
AU  - Fukuchi M
FAU - Shi, M J
AU  - Shi MJ
FAU - Stavnezer, J
AU  - Stavnezer J
FAU - Kawabata, M
AU  - Kawabata M
FAU - Miyazono, K
AU  - Miyazono K
FAU - Ito, Y
AU  - Ito Y
LA  - eng
GR  - R01 AI23283/AI/NIAID NIH HHS/United States
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - J Biol Chem
JT  - The Journal of biological chemistry
JID - 2985121R
RN  - 0 (Bone Morphogenetic Proteins)
RN  - 0 (DNA-Binding Proteins)
RN  - 0 (Immunoglobulins)
RN  - 0 (Receptors, Transforming Growth Factor beta)
RN  - 0 (Smad3 Protein)
RN  - 0 (Trans-Activators)
RN  - 0 (Transcription Factor AP-2)
RN  - 0 (Transcription Factors)
RN  - 0 (Transforming Growth Factor beta)
RN  - EC 2.7.11.1 (Protein-Serine-Threonine Kinases)
RN  - EC 2.7.11.30 (Activin Receptors, Type I)
RN  - EC 2.7.11.30 (Receptor, Transforming Growth Factor-beta Type I)
SB  - IM
MH  - *Activin Receptors, Type I
MH  - Bone Morphogenetic Proteins/*physiology
MH  - DNA-Binding Proteins/*metabolism
MH  - Germ Cells
MH  - Immunoglobulins/*genetics
MH  - Promoter Regions, Genetic
MH  - Protein Binding
MH  - Protein-Serine-Threonine Kinases/metabolism
MH  - Receptor, Transforming Growth Factor-beta Type I
MH  - Receptors, Transforming Growth Factor beta/metabolism
MH  - Signal Transduction
MH  - Smad3 Protein
MH  - Trans-Activators/metabolism
MH  - Transcription Factor AP-2
MH  - Transcription Factors/*metabolism
MH  - *Transcriptional Activation
MH  - Transforming Growth Factor beta/*physiology
EDAT- 1999/10/26 00:00
MHDA- 1999/10/26 00:01
CRDT- 1999/10/26 00:00
PHST- 1999/10/26 00:00 [pubmed]
PHST- 1999/10/26 00:01 [medline]
PHST- 1999/10/26 00:00 [entrez]
AID - 10.1074/jbc.274.44.31577 [doi]
PST - ppublish
SO  - J Biol Chem. 1999 Oct 29;274(44):31577-82. doi: 10.1074/jbc.274.44.31577.