PMID- 10531361
OWN - NLM
STAT- MEDLINE
DCOM- 19991216
LR  - 20190508
IS  - 0021-9258 (Print)
IS  - 0021-9258 (Linking)
VI  - 274
IP  - 44
DP  - 1999 Oct 29
TI  - Visinin-like protein (VILIP) is a neuron-specific calcium-dependent
      double-stranded RNA-binding protein.
PG  - 31571-6
AB  - Double-stranded RNA-binding proteins function in regulating the stability,
      translation, and localization of specific mRNAs. In this study, we have
      demonstrated that the neuron-specific, calcium-binding protein, visinin-like
      protein (VILIP) contains one double-stranded RNA-binding domain, a protein motif 
      conserved among many double-stranded RNA-binding proteins. We showed that VILIP
      can specifically bind double-stranded RNA, and this interaction specifically
      requires the presence of calcium. Mobility shift studies indicated that VILIP
      binds double-stranded RNA as a single protein-RNA complex with an apparent
      equilibrium dissociation constant of 9.0 x 10(-6) M. To our knowledge, VILIP is
      the first double-stranded RNA-binding protein shown to be calcium-dependent.
      Furthermore, VILIP specifically binds the 3'-untranslated region of the
      neurotrophin receptor, trkB, an mRNA localized to hippocampal dendrites in an
      activity-dependent manner. Given that VILIP is also expressed in the hippocampus,
      these data suggest that VILIP may employ a novel, calcium-dependent mechanism to 
      regulate its binding to important localized mRNAs in the central nervous system.
FAU - Mathisen, P M
AU  - Mathisen PM
AD  - Department of Immunology, Lerner Research Institute, The Cleveland Clinic
      Foundation, Cleveland, Ohio 44195, USA. mathisp@ccf.org
FAU - Johnson, J M
AU  - Johnson JM
FAU - Kawczak, J A
AU  - Kawczak JA
FAU - Tuohy, V K
AU  - Tuohy VK
LA  - eng
GR  - NS36054/NS/NINDS NIH HHS/United States
GR  - RG2768/RG/CSR NIH HHS/United States
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - J Biol Chem
JT  - The Journal of biological chemistry
JID - 2985121R
RN  - 0 (3' Untranslated Regions)
RN  - 0 (Calcium-Binding Proteins)
RN  - 0 (Nerve Tissue Proteins)
RN  - 0 (Neurocalcin)
RN  - 0 (RNA, Double-Stranded)
RN  - 0 (RNA-Binding Proteins)
RN  - 0 (Receptors, Calcium-Sensing)
RN  - 0 (Vsnl1 protein, mouse)
RN  - EC 2.7.10.1 (Receptor, trkB)
RN  - SY7Q814VUP (Calcium)
SB  - IM
MH  - 3' Untranslated Regions
MH  - Amino Acid Sequence
MH  - Animals
MH  - Brain Chemistry
MH  - Calcium/*metabolism
MH  - Calcium-Binding Proteins/*metabolism
MH  - Hippocampus/cytology
MH  - Mice
MH  - Molecular Sequence Data
MH  - Nerve Tissue Proteins/*metabolism
MH  - Neurocalcin
MH  - Neurons/chemistry/metabolism
MH  - Protein Binding
MH  - Protein Structure, Tertiary
MH  - RNA, Double-Stranded/*metabolism
MH  - RNA-Binding Proteins/*metabolism
MH  - Receptor, trkB/genetics
MH  - *Receptors, Calcium-Sensing
MH  - Sequence Homology, Amino Acid
EDAT- 1999/10/26 00:00
MHDA- 1999/10/26 00:01
CRDT- 1999/10/26 00:00
PHST- 1999/10/26 00:00 [pubmed]
PHST- 1999/10/26 00:01 [medline]
PHST- 1999/10/26 00:00 [entrez]
AID - 10.1074/jbc.274.44.31571 [doi]
PST - ppublish
SO  - J Biol Chem. 1999 Oct 29;274(44):31571-6. doi: 10.1074/jbc.274.44.31571.