PMID- 10531356
OWN - NLM
STAT- MEDLINE
DCOM- 19991216
LR  - 20190614
IS  - 0021-9258 (Print)
IS  - 0021-9258 (Linking)
VI  - 274
IP  - 44
DP  - 1999 Oct 29
TI  - The human homologue of the yeast proteins Skb1 and Hsl7p interacts with Jak
      kinases and contains protein methyltransferase activity.
PG  - 31531-42
AB  - To expand our understanding of the role of Jak2 in cellular signaling, we used
      the yeast two-hybrid system to identify Jak2-interacting proteins. One of the
      clones identified represents a human homologue of the Schizosaccaromyces pombe
      Shk1 kinase-binding protein 1, Skb1, and the protein encoded by the Saccharomyces
      cerevisiae HSL7 (histone synthetic lethal 7) gene. Since no functional motifs or 
      biochemical activities for this protein or its homologues had been reported, we
      sought to determine a biochemical function for this human protein. We demonstrate
      that this protein is a protein methyltransferase. This protein, designated JBP1
      (Jak-binding protein 1), and its homologues contain motifs conserved among
      protein methyltransferases. JBP1 can be cross-linked to radiolabeled
      S-adenosylmethionine (AdoMet) and methylates histones (H2A and H4) and myelin
      basic protein. Mutants containing substitutions within a conserved region likely 
      to be involved in AdoMet binding exhibit little or no activity. We mapped the
      JBP1 gene to chromosome 14q11.2-21. In addition, JBP1 co-immunoprecipitates with 
      several other proteins, which serve as methyl group acceptors and which may
      represent physiological targets of this methyltransferase. Messenger RNA for JBP1
      is widely expressed in human tissues. We have also identified and sequenced a
      homologue of JBP1 in Drosophila melanogaster. This report provides a clue to the 
      biochemical function for this conserved protein and suggests that protein
      methyltransferases may have a role in cellular signaling.
FAU - Pollack, B P
AU  - Pollack BP
AD  - Department of Molecular Genetics, University of Medicine and Dentistry of New
      Jersey, Robert Wood Johnson Medical School, Piscataway, New Jersey 08854-5635,
      USA.
FAU - Kotenko, S V
AU  - Kotenko SV
FAU - He, W
AU  - He W
FAU - Izotova, L S
AU  - Izotova LS
FAU - Barnoski, B L
AU  - Barnoski BL
FAU - Pestka, S
AU  - Pestka S
LA  - eng
SI  - GENBANK/AF167572
SI  - GENBANK/AF167573
SI  - GENBANK/AF167574
GR  - 2T32AI07403/AI/NIAID NIH HHS/United States
GR  - R01 AI36450/AI/NIAID NIH HHS/United States
GR  - R01-CA46465/CA/NCI NIH HHS/United States
GR  - etc.
PT  - Comparative Study
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - J Biol Chem
JT  - The Journal of biological chemistry
JID - 2985121R
RN  - 0 (Carrier Proteins)
RN  - 0 (Drosophila Proteins)
RN  - 0 (Histones)
RN  - 0 (Proto-Oncogene Proteins)
RN  - 0 (Saccharomyces cerevisiae Proteins)
RN  - 0 (Schizosaccharomyces pombe Proteins)
RN  - 0 (Skb1 protein, S pombe)
RN  - EC 2.1.1.- (Methyltransferases)
RN  - EC 2.1.1.- (Protein Methyltransferases)
RN  - EC 2.1.1.319 (PRMT5 protein, human)
RN  - EC 2.1.1.319 (Protein-Arginine N-Methyltransferases)
RN  - EC 2.1.1.319 (csul protein, Drosophila)
RN  - EC 2.1.1.320 (HSL7 protein, S cerevisiae)
RN  - EC 2.7.- (Protein Kinases)
RN  - EC 2.7.10.1 (Protein-Tyrosine Kinases)
RN  - EC 2.7.10.2 (JAK2 protein, human)
RN  - EC 2.7.10.2 (Janus Kinase 2)
SB  - IM
MH  - Amino Acid Sequence
MH  - Carrier Proteins/genetics
MH  - Chromosome Mapping
MH  - Chromosomes, Human, Pair 14
MH  - Cloning, Molecular
MH  - Drosophila Proteins
MH  - Histones/metabolism
MH  - Humans
MH  - In Situ Hybridization, Fluorescence
MH  - Janus Kinase 2
MH  - Methylation
MH  - *Methyltransferases
MH  - Molecular Sequence Data
MH  - Point Mutation
MH  - Precipitin Tests
MH  - Protein Binding
MH  - Protein Kinases/genetics
MH  - Protein Methyltransferases/genetics/*metabolism
MH  - Protein-Arginine N-Methyltransferases
MH  - Protein-Tyrosine Kinases/*metabolism
MH  - *Proto-Oncogene Proteins
MH  - *Saccharomyces cerevisiae Proteins
MH  - *Schizosaccharomyces pombe Proteins
MH  - Sequence Homology, Amino Acid
MH  - Substrate Specificity
MH  - Two-Hybrid System Techniques
EDAT- 1999/10/26 00:00
MHDA- 1999/10/26 00:01
CRDT- 1999/10/26 00:00
PHST- 1999/10/26 00:00 [pubmed]
PHST- 1999/10/26 00:01 [medline]
PHST- 1999/10/26 00:00 [entrez]
AID - 10.1074/jbc.274.44.31531 [doi]
PST - ppublish
SO  - J Biol Chem. 1999 Oct 29;274(44):31531-42. doi: 10.1074/jbc.274.44.31531.