PMID- 10531061
OWN - NLM
STAT- MEDLINE
DCOM- 19991105
LR  - 20190619
IS  - 0036-8075 (Print)
IS  - 0036-8075 (Linking)
VI  - 286
IP  - 5440
DP  - 1999 Oct 22
TI  - Light-independent role of CRY1 and CRY2 in the mammalian circadian clock.
PG  - 768-71
AB  - Cryptochrome (CRY), a photoreceptor for the circadian clock in Drosophila, binds 
      to the clock component TIM in a light-dependent fashion and blocks its function. 
      In mammals, genetic evidence suggests a role for CRYs within the clock, distinct 
      from hypothetical photoreceptor functions. Mammalian CRY1 and CRY2 are here shown
      to act as light-independent inhibitors of CLOCK-BMAL1, the activator driving Per1
      transcription. CRY1 or CRY2 (or both) showed light-independent interactions with 
      CLOCK and BMAL1, as well as with PER1, PER2, and TIM. Thus, mammalian CRYs act as
      light-independent components of the circadian clock and probably regulate Per1
      transcriptional cycling by contacting both the activator and its feedback
      inhibitors.
FAU - Griffin, E A Jr
AU  - Griffin EA Jr
AD  - Department of Neurobiology, Harvard Medical School, Boston, MA 02115 USA.
FAU - Staknis, D
AU  - Staknis D
FAU - Weitz, C J
AU  - Weitz CJ
LA  - eng
GR  - MH-59943/MH/NIMH NIH HHS/United States
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - Science
JT  - Science (New York, N.Y.)
JID - 0404511
RN  - 0 (ARNTL Transcription Factors)
RN  - 0 (ARNTL protein, human)
RN  - 0 (Arntl protein, mouse)
RN  - 0 (Basic Helix-Loop-Helix Transcription Factors)
RN  - 0 (CRY1 protein, human)
RN  - 0 (CRY2 protein, human)
RN  - 0 (Cell Cycle Proteins)
RN  - 0 (Cry1 protein, mouse)
RN  - 0 (Cry2 protein, mouse)
RN  - 0 (Cryptochromes)
RN  - 0 (Drosophila Proteins)
RN  - 0 (Eye Proteins)
RN  - 0 (Flavoproteins)
RN  - 0 (Intracellular Signaling Peptides and Proteins)
RN  - 0 (Nuclear Proteins)
RN  - 0 (PER1 protein, human)
RN  - 0 (PER2 protein, human)
RN  - 0 (Per1 protein, mouse)
RN  - 0 (Per2 protein, mouse)
RN  - 0 (Period Circadian Proteins)
RN  - 0 (Receptors, G-Protein-Coupled)
RN  - 0 (TIMELESS protein, human)
RN  - 0 (Timeless protein, mouse)
RN  - 0 (Trans-Activators)
RN  - 0 (Transcription Factors)
RN  - 0 (cry protein, Drosophila)
RN  - EC 2.3.1.48 (CLOCK Proteins)
RN  - EC 2.3.1.48 (CLOCK protein, human)
RN  - EC 2.3.1.48 (Clock protein, mouse)
SB  - IM
MH  - 3T3 Cells
MH  - ARNTL Transcription Factors
MH  - Animals
MH  - Basic Helix-Loop-Helix Transcription Factors
MH  - *Biological Clocks
MH  - CLOCK Proteins
MH  - Cell Cycle Proteins
MH  - Cells, Cultured
MH  - *Circadian Rhythm
MH  - Cryptochromes
MH  - Dimerization
MH  - *Drosophila Proteins
MH  - *Eye Proteins
MH  - Flavoproteins/metabolism/*physiology
MH  - *Gene Expression Regulation
MH  - Genes, Reporter
MH  - Helix-Loop-Helix Motifs
MH  - Humans
MH  - Intracellular Signaling Peptides and Proteins
MH  - *Light
MH  - Mice
MH  - Nuclear Proteins/antagonists & inhibitors/*genetics/metabolism
MH  - Period Circadian Proteins
MH  - *Photoreceptor Cells, Invertebrate
MH  - Receptors, G-Protein-Coupled
MH  - Trans-Activators/antagonists & inhibitors/metabolism
MH  - Transcription Factors/antagonists & inhibitors/metabolism
MH  - Transcriptional Activation
MH  - Transfection
EDAT- 1999/10/26 09:00
MHDA- 2001/03/28 10:01
CRDT- 1999/10/26 09:00
PHST- 1999/10/26 09:00 [pubmed]
PHST- 2001/03/28 10:01 [medline]
PHST- 1999/10/26 09:00 [entrez]
AID - 7924 [pii]
AID - 10.1126/science.286.5440.768 [doi]
PST - ppublish
SO  - Science. 1999 Oct 22;286(5440):768-71. doi: 10.1126/science.286.5440.768.