PMID- 10531035
OWN - NLM
STAT- MEDLINE
DCOM- 19991206
LR  - 20161124
IS  - 0960-9822 (Print)
IS  - 0960-9822 (Linking)
VI  - 9
IP  - 20
DP  - 1999 Oct 21
TI  - Identification of a family of human F-box proteins.
PG  - 1177-9
AB  - F-box proteins are an expanding family of eukaryotic proteins characterized by an
      approximately 40 aminoacid motif, the F box (so named because cyclin F was one of
      the first proteins in which this motif was identified) [1]. Some F-box proteins
      have been shown to be critical for the controlled degradation of cellular
      regulatory proteins [2] [3]. In fact, F-box proteins are one of the four subunits
      of ubiquitin protein ligases called SCFs. The other three subunits are the Skp1
      protein; one of the cullin proteins (Cul1 in metazoans and Cdc53 or Cul A in the 
      yeast Saccharomyces cerevisiae); and the recently identified Roc1 protein (also
      called Rbx1 or Hrt1). SCF ligases bring ubiquitin conjugating enzymes (either
      Ubc3 or Ubc4) to substrates that are specifically recruited by the different
      F-box proteins. The need for high substrate specificity and the large number of
      known F-box proteins in yeast and worms [2] [4] suggest the existence of a large 
      family of mammalian F-box proteins. Using Skp1 as a bait in a yeast two-hybrid
      screen and by searching DNA databases, we identified a family of 26 human F-box
      proteins, 25 of which were novel. Some of these proteins contained WD-40 domains 
      or leucine-rich repeats; others contained either different protein-protein
      interaction modules or no recognizable motifs. We have named the F-box proteins
      that contain WD-40 domains Fbws, those containing leucine-rich repeats, Fbls, and
      the remaining ones Fbxs. We have further characterized representative members of 
      these three classes of F-box proteins.
FAU - Cenciarelli, C
AU  - Cenciarelli C
AD  - Department of Pathology, Kaplan Comprehensive Cancer Center, MSB 548, New York
      University School of Medicine 550 First Avenue, New York, New York, 10016, USA.
FAU - Chiaur, D S
AU  - Chiaur DS
FAU - Guardavaccaro, D
AU  - Guardavaccaro D
FAU - Parks, W
AU  - Parks W
FAU - Vidal, M
AU  - Vidal M
FAU - Pagano, M
AU  - Pagano M
LA  - eng
SI  - GENBANK/AF129530
SI  - GENBANK/AF129531
SI  - GENBANK/AF129532
SI  - GENBANK/AF129533
SI  - GENBANK/AF129534
SI  - GENBANK/AF129535
SI  - GENBANK/AF129536
SI  - GENBANK/AF129537
SI  - GENBANK/AF174589
SI  - GENBANK/AF174590
SI  - GENBANK/AF174591
SI  - GENBANK/AF174592
SI  - GENBANK/AF174593
SI  - GENBANK/AF174594
SI  - GENBANK/AF174595
SI  - GENBANK/AF174596
SI  - GENBANK/AF174597
SI  - GENBANK/AF174598
SI  - GENBANK/AF174599
SI  - GENBANK/AF174600
SI  - GENBANK/AF174601
SI  - GENBANK/AF174602
SI  - GENBANK/AF174603
SI  - GENBANK/AF174604
SI  - GENBANK/AF174605
SI  - GENBANK/AF174606
SI  - GENBANK/AF176022
GR  - R01 CA76584/CA/NCI NIH HHS/United States
GR  - R01 GM57587/GM/NIGMS NIH HHS/United States
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, Non-P.H.S.
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - England
TA  - Curr Biol
JT  - Current biology : CB
JID - 9107782
RN  - 0 (Proteins)
RN  - 0 (Ubiquitins)
RN  - EC 2.3.2.27 (SKP Cullin F-Box Protein Ligases)
RN  - EC 6.3.2.- (Peptide Synthases)
SB  - IM
MH  - Amino Acid Motifs
MH  - Amino Acid Sequence
MH  - Animals
MH  - HeLa Cells
MH  - Humans
MH  - Molecular Sequence Data
MH  - Peptide Synthases/metabolism
MH  - Proteins/*chemistry/*classification/genetics
MH  - SKP Cullin F-Box Protein Ligases
MH  - Sequence Homology, Amino Acid
MH  - Two-Hybrid System Techniques
MH  - Ubiquitins/metabolism
EDAT- 1999/10/26 00:00
MHDA- 1999/10/26 00:01
CRDT- 1999/10/26 00:00
PHST- 1999/10/26 00:00 [pubmed]
PHST- 1999/10/26 00:01 [medline]
PHST- 1999/10/26 00:00 [entrez]
AID - S0960-9822(00)80020-2 [pii]
AID - 10.1016/S0960-9822(00)80020-2 [doi]
PST - ppublish
SO  - Curr Biol. 1999 Oct 21;9(20):1177-9. doi: 10.1016/S0960-9822(00)80020-2.