PMID- 10529385
OWN - NLM
STAT- MEDLINE
DCOM- 19991207
LR  - 20120625
IS  - 0006-291X (Print)
IS  - 0006-291X (Linking)
VI  - 264
IP  - 2
DP  - 1999 Oct 22
TI  - Cloning and characterization of a novel p21(Cip1/Waf1)-interacting zinc finger
      protein, ciz1.
PG  - 457-64
AB  - p21(Cip1/Waf1) inhibits cell-cycle progression by binding to G1 cyclin/CDK
      complexes and proliferating cell nuclear antigen (PCNA) through its N- and
      C-terminal domains, respectively. Here, we report a novel
      p21(Cip1/Waf1)-interacting protein, Ciz1 (for Cip1 interacting zinc finger
      protein), which contains polyglutamine repeats and glutamine-rich region in the
      N-terminus as well as three zinc-finger motifs and one MH3 (matrin 3-homologous
      domain 3) in the C-terminal region. Ciz1 bound to the N-terminal, the
      CDK2-interacting part of p21(Cip1/Waf1), and the interaction was disrupted by the
      overexpression of CDK2. A region of about 150 amino acids containing the first
      zinc-finger motif in Ciz1 was the binding site for p21(Cip1/Waf1). When Ciz1 and 
      p21(Cip1/Waf1) were individually overexpressed in U2-OS cells, they mostly
      localized in the nucleus. However, coexpression of Ciz1 induced cytoplasmic
      distribution of p21(Cip1/Waf1). These data indicate that Ciz1 is a unique nuclear
      protein that regulates the cellular localization of p21(Cip1/Waf1).
CI  - Copyright 1999 Academic Press.
FAU - Mitsui, K
AU  - Mitsui K
AD  - Institute of Life Science, Kurume University, Aikawa-machi 2432-3, Kurume,
      839-0861, Japan.
FAU - Matsumoto, A
AU  - Matsumoto A
FAU - Ohtsuka, S
AU  - Ohtsuka S
FAU - Ohtsubo, M
AU  - Ohtsubo M
FAU - Yoshimura, A
AU  - Yoshimura A
LA  - eng
SI  - GENBANK/AB030835
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Biochem Biophys Res Commun
JT  - Biochemical and biophysical research communications
JID - 0372516
RN  - 0 (CDKN1A protein, human)
RN  - 0 (Cyclin-Dependent Kinase Inhibitor p21)
RN  - 0 (Cyclins)
RN  - 0 (DNA, Complementary)
RN  - 0 (Enzyme Inhibitors)
RN  - 0 (RNA, Messenger)
RN  - EC 2.7.11.1 (Protein-Serine-Threonine Kinases)
RN  - EC 2.7.11.22 (CDC2-CDC28 Kinases)
RN  - EC 2.7.11.22 (CDK2 protein, human)
RN  - EC 2.7.11.22 (Cyclin-Dependent Kinase 2)
RN  - EC 2.7.11.22 (Cyclin-Dependent Kinases)
SB  - IM
MH  - Amino Acid Sequence
MH  - Binding Sites
MH  - *CDC2-CDC28 Kinases
MH  - Cell Line
MH  - Cell Nucleus/metabolism
MH  - Cloning, Molecular
MH  - Cyclin-Dependent Kinase 2
MH  - Cyclin-Dependent Kinase Inhibitor p21
MH  - Cyclin-Dependent Kinases/chemistry/metabolism
MH  - Cyclins/*chemistry/genetics
MH  - DNA, Complementary/chemistry/isolation & purification
MH  - Enzyme Inhibitors/*chemistry/metabolism
MH  - Gene Library
MH  - Humans
MH  - Molecular Sequence Data
MH  - Mutation
MH  - Protein-Serine-Threonine Kinases/chemistry/metabolism
MH  - RNA, Messenger/biosynthesis
MH  - Zinc Fingers
EDAT- 1999/10/26 00:00
MHDA- 1999/10/26 00:01
CRDT- 1999/10/26 00:00
PHST- 1999/10/26 00:00 [pubmed]
PHST- 1999/10/26 00:01 [medline]
PHST- 1999/10/26 00:00 [entrez]
AID - 10.1006/bbrc.1999.1516 [doi]
AID - S0006-291X(99)91516-4 [pii]
PST - ppublish
SO  - Biochem Biophys Res Commun. 1999 Oct 22;264(2):457-64. doi:
      10.1006/bbrc.1999.1516.