PMID- 10529272
OWN - NLM
STAT- MEDLINE
DCOM- 19991206
LR  - 20190513
IS  - 0006-3363 (Print)
IS  - 0006-3363 (Linking)
VI  - 61
IP  - 5
DP  - 1999 Nov
TI  - Round spermatid-specific transcription of the mouse SP-10 gene is mediated by a
      294-base pair proximal promoter.
PG  - 1256-66
AB  - Spermiogenesis is the terminal phase of male germ cell differentiation during
      which haploid spermatids engage in coordinate expression of a number of
      testis-specific genes, including those specifying acrosomal proteins. To begin to
      understand the transcriptional regulation during acrosomal biogenesis, we
      initiated promoter analysis of the gene encoding the acrosomal protein SP-10.
      SP-10 was previously shown to be transcribed within Golgi-phase round spermatids 
      in the human. The present study characterizes SP-10 gene expression during
      spermiogenesis in the mouse and identifies regions of the mouse SP-10 (mSP-10)
      promoter that are capable of driving round spermatid-specific transcription in
      vivo. Expression of mSP-10 mRNA was initiated in early round spermatids
      coincident with acrosomal biogenesis and was terminated prior to nuclear
      elongation. The core promoter of mSP-10 lacked a TATA box but contained a
      canonical initiator (Inr) element surrounding the transcription start site. Using
      transgenic mice, we showed that the -408 to +28-base pair (bp) or the -266 to
      +28-bp mSP-10 5' flanking region is sufficient to direct round spermatid-specific
      expression of a green fluorescent protein reporter gene. On the other hand, the
      -91 to +28-bp mSP-10 gene fragment lacked promoter activity in vivo. This is the 
      first functional characterization of a testis-specific gene promoter active in
      early round spermatids.
FAU - Reddi, P P
AU  - Reddi PP
AD  - Department of Cell Biology, University of Virginia, Charlottesville, Virginia
      22908, USA. ppr5s@virginia.edu
FAU - Flickinger, C J
AU  - Flickinger CJ
FAU - Herr, J C
AU  - Herr JC
LA  - eng
SI  - GENBANK/AF133710
GR  - R29-HD36239-01/HD/NICHD NIH HHS/United States
GR  - U54-HD28934/HD/NICHD NIH HHS/United States
GR  - U54-HD29099/HD/NICHD NIH HHS/United States
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - Biol Reprod
JT  - Biology of reproduction
JID - 0207224
RN  - 0 (ACRV1 protein, human)
RN  - 0 (Acrv1 protein, mouse)
RN  - 0 (Antigens)
RN  - 0 (DNA Primers)
RN  - 0 (Gonadal Steroid Hormones)
RN  - 0 (Membrane Proteins)
RN  - 0 (Proteins)
RN  - 9007-49-2 (DNA)
SB  - IM
MH  - *Acrosome
MH  - Animals
MH  - *Antigens
MH  - Base Pairing
MH  - Base Sequence
MH  - Cloning, Molecular
MH  - DNA/chemistry
MH  - DNA Primers
MH  - Genes, Reporter/genetics
MH  - Genome
MH  - Genomic Library
MH  - *Gonadal Steroid Hormones
MH  - In Situ Hybridization
MH  - Male
MH  - Membrane Proteins
MH  - Mice
MH  - Mice, Transgenic
MH  - Molecular Sequence Data
MH  - Promoter Regions, Genetic/*genetics
MH  - *Protein Biosynthesis
MH  - Proteins/*genetics
MH  - Reverse Transcriptase Polymerase Chain Reaction
MH  - Spermatids/*metabolism/*ultrastructure
MH  - Testis/growth & development/metabolism
MH  - Transcription, Genetic/*physiology
EDAT- 1999/10/21 00:00
MHDA- 1999/10/21 00:01
CRDT- 1999/10/21 00:00
PHST- 1999/10/21 00:00 [pubmed]
PHST- 1999/10/21 00:01 [medline]
PHST- 1999/10/21 00:00 [entrez]
AID - 10.1095/biolreprod61.5.1256 [doi]
PST - ppublish
SO  - Biol Reprod. 1999 Nov;61(5):1256-66. doi: 10.1095/biolreprod61.5.1256.