PMID- 10528213
OWN - NLM
STAT- MEDLINE
DCOM- 19991119
LR  - 20101118
IS  - 0022-1767 (Print)
IS  - 0022-1767 (Linking)
VI  - 163
IP  - 9
DP  - 1999 Nov 1
TI  - L-selectin ligands expressed by human leukocytes are HECA-452 antibody-defined
      carbohydrate epitopes preferentially displayed by P-selectin glycoprotein
      ligand-1.
PG  - 5070-8
AB  - Leukocytes express L-selectin ligands critical for leukocyte-leukocyte
      interactions at sites of inflammation. The predominant leukocyte L-selectin
      ligand is P-selectin glycoprotein ligand-1 (PSGL-1), which displays appropriate
      sialyl Lewis x (sLex)-like carbohydrate determinants for L-selectin recognition. 
      Among the sLex-like determinants expressed by human leukocytes is a unique
      carbohydrate epitope defined by the HECA-452 mAb. The HECA-452 Ag is a critical
      component of L-selectin ligands expressed by vascular endothelial cells. However,
      HECA-452 Ag expression on human leukocyte L-selectin ligands has not been
      assessed. In this study, the HECA-452 mAb blocked 88-99% of neutrophil rolling
      on, or attachment to, adherent cells expressing L-selectin in multiple
      experimental systems. A function-blocking anti-PSGL-1 mAb also inhibited
      L-selectin binding to neutrophils by 89-98%. In addition, the HECA-452 and
      anti-PSGL-1 mAbs blocked the majority of P-selectin binding to neutrophils.
      Western blot analysis revealed that PSGL-1 immunoprecipitated from neutrophils
      displayed HECA-452 mAb-reactive determinants and that PSGL-1 was the predominant 
      scaffold for HECA-452 Ag display. Leukocyte L-selectin ligands also contained
      sulfated determinants since culturing ligand-bearing cells with NaClO3 abrogated 
      L-selectin binding. Consistent with this, human neutrophils expressed mRNA
      encoding five different sulfotransferases associated with the generation of
      selectin ligands: CHST1, CHST2, CHST3, TPST1, and HEC-GlcNAc6ST. Therefore, the
      HECA-452-defined carbohydrate determinant displayed on PSGL-1 represented the
      predominant L-selectin and P-selectin ligand expressed by neutrophils.
FAU - Tu, L
AU  - Tu L
AD  - Department of Immunology, Duke University Medical Center, Durham, NC 27710, USA.
FAU - Murphy, P G
AU  - Murphy PG
FAU - Li, X
AU  - Li X
FAU - Tedder, T F
AU  - Tedder TF
LA  - eng
GR  - CA54464/CA/NCI NIH HHS/United States
GR  - CA81776/CA/NCI NIH HHS/United States
GR  - HL50985/HL/NHLBI NIH HHS/United States
PT  - Journal Article
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - J Immunol
JT  - Journal of immunology (Baltimore, Md. : 1950)
JID - 2985117R
RN  - 0 (Antibodies, Blocking)
RN  - 0 (Antibodies, Monoclonal)
RN  - 0 (Antigens, Differentiation, T-Lymphocyte)
RN  - 0 (Antigens, Neoplasm)
RN  - 0 (CTAGE1 protein, human)
RN  - 0 (Carbohydrates)
RN  - 0 (Epitopes)
RN  - 0 (Ligands)
RN  - 0 (Membrane Glycoproteins)
RN  - 0 (P-Selectin)
RN  - 0 (P-selectin ligand protein)
RN  - 126880-86-2 (L-Selectin)
RN  - EC 2.4.1.- (Fucosyltransferases)
RN  - EC 2.4.1.152 (galactoside 3-fucosyltransferase)
RN  - EC 2.8.2.- (Sulfotransferases)
SB  - AIM
SB  - IM
MH  - Animals
MH  - Antibodies, Blocking/pharmacology
MH  - Antibodies, Monoclonal/*metabolism/pharmacology
MH  - Antigens, Differentiation, T-Lymphocyte
MH  - Antigens, Neoplasm
MH  - Blood Circulation/immunology/physiology
MH  - COS Cells
MH  - *Carbohydrate Metabolism
MH  - Carbohydrates/immunology
MH  - Cell Movement/immunology/physiology
MH  - Epitopes/*metabolism
MH  - Fucosyltransferases/genetics
MH  - HL-60 Cells
MH  - Humans
MH  - L-Selectin/*biosynthesis/metabolism
MH  - Leukocytes/*metabolism
MH  - Ligands
MH  - Membrane Glycoproteins/immunology/*metabolism
MH  - Mice
MH  - Neutrophils/immunology/metabolism/physiology
MH  - P-Selectin/immunology/*metabolism
MH  - Sulfotransferases/biosynthesis/genetics
MH  - Transfection
EDAT- 1999/10/21 00:00
MHDA- 1999/10/21 00:01
CRDT- 1999/10/21 00:00
PHST- 1999/10/21 00:00 [pubmed]
PHST- 1999/10/21 00:01 [medline]
PHST- 1999/10/21 00:00 [entrez]
AID - ji_v163n9p5070 [pii]
PST - ppublish
SO  - J Immunol. 1999 Nov 1;163(9):5070-8.