PMID- 10528209
OWN - NLM
STAT- MEDLINE
DCOM- 19991119
LR  - 20081121
IS  - 0022-1767 (Print)
IS  - 0022-1767 (Linking)
VI  - 163
IP  - 9
DP  - 1999 Nov 1
TI  - A novel LPS-inducible C-type lectin is a transcriptional target of NF-IL6 in
      macrophages.
PG  - 5039-48
AB  - C-type lectins serve multiple functions through recognizing carbohydrate chains. 
      Here we report a novel C-type lectin, macrophage-inducible C-type lectin
      (Mincle), as a downstream target of NF-IL6 in macrophages. NF-IL6 belongs to the 
      CCAAT/enhancer binding protein (C/EBP) of transcription factors and plays a
      crucial role in activated macrophages. However, what particular genes are
      regulated by NF-IL6 has been poorly defined in macrophages. Identification of
      downstream targets is required to elucidate the function of NF-IL6 in more
      detail. To identify downstream genes of NF-IL6, we screened a subtraction library
      constructed from wild-type and NF-IL6-deficient peritoneal macrophages and
      isolated Mincle that exhibits the highest homology to the members of group II
      C-type lectins. Mincle mRNA expression was strongly induced in response to
      several inflammatory stimuli, such as LPS, TNF-alpha, IL-6, and IFN-gamma in
      wild-type macrophages. In contrast, NF-IL6-deficient macrophages displayed a much
      lower level of Mincle mRNA induction following treatment with these inflammatory 
      reagents. The mouse Mincle proximal promoter region contains an indispensable
      NF-IL6 binding element, demonstrating that Mincle is a direct target of NF-IL6.
      The Mincle gene locus was mapped at 0.6 centiMorgans proximal to CD4 on mouse
      chromosome 6.
FAU - Matsumoto, M
AU  - Matsumoto M
AD  - Department of Host Defense, Research Institute for Microbial Diseases, Osaka
      University, Japan.
FAU - Tanaka, T
AU  - Tanaka T
FAU - Kaisho, T
AU  - Kaisho T
FAU - Sanjo, H
AU  - Sanjo H
FAU - Copeland, N G
AU  - Copeland NG
FAU - Gilbert, D J
AU  - Gilbert DJ
FAU - Jenkins, N A
AU  - Jenkins NA
FAU - Akira, S
AU  - Akira S
LA  - eng
SI  - GENBANK/AB024717
SI  - GENBANK/AB024718
SI  - GENBANK/AB024719
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - J Immunol
JT  - Journal of immunology (Baltimore, Md. : 1950)
JID - 2985117R
RN  - 0 (CCAAT-Enhancer-Binding Proteins)
RN  - 0 (Clecsf8 protein, mouse)
RN  - 0 (DNA-Binding Proteins)
RN  - 0 (Lectins)
RN  - 0 (Lectins, C-Type)
RN  - 0 (Lipopolysaccharides)
RN  - 0 (Membrane Proteins)
RN  - 0 (Nuclear Proteins)
RN  - 0 (Transcription Factors)
SB  - AIM
SB  - IM
MH  - Animals
MH  - CCAAT-Enhancer-Binding Proteins
MH  - Chromosome Mapping
MH  - Cloning, Molecular
MH  - DNA-Binding Proteins/deficiency/genetics/metabolism/*physiology
MH  - Female
MH  - Gene Expression Regulation/immunology
MH  - Lectins/*biosynthesis/*genetics/metabolism
MH  - *Lectins, C-Type
MH  - Lipopolysaccharides/*pharmacology
MH  - Macrophages, Peritoneal/immunology/*metabolism
MH  - Male
MH  - Membrane Proteins/*biosynthesis/*genetics/metabolism
MH  - Mice
MH  - Mice, Inbred C57BL
MH  - Mice, Knockout
MH  - Nuclear Proteins/deficiency/genetics/metabolism/*physiology
MH  - Promoter Regions, Genetic/immunology
MH  - Protein Binding/genetics/immunology
MH  - Transcription Factors/deficiency/genetics/metabolism/*physiology
MH  - Transcription, Genetic/*immunology
EDAT- 1999/10/21 00:00
MHDA- 1999/10/21 00:01
CRDT- 1999/10/21 00:00
PHST- 1999/10/21 00:00 [pubmed]
PHST- 1999/10/21 00:01 [medline]
PHST- 1999/10/21 00:00 [entrez]
AID - ji_v163n9p5039 [pii]
PST - ppublish
SO  - J Immunol. 1999 Nov 1;163(9):5039-48.