PMID- 10528200
OWN - NLM
STAT- MEDLINE
DCOM- 19991119
LR  - 20171116
IS  - 0022-1767 (Print)
IS  - 0022-1767 (Linking)
VI  - 163
IP  - 9
DP  - 1999 Nov 1
TI  - Immune-associated nucleotide-1 (IAN-1) is a thymic selection marker and defines a
      novel gene family conserved in plants.
PG  - 4960-9
AB  - Positive selection of thymocytes is a complex and crucial event in T cell
      development that is characterized by cell death rescue, commitment toward the
      helper or cytotoxic lineage, and functional maturation of thymocytes bearing an
      appropriate TCR. To search for novel genes involved in this process, we compared 
      gene expression patterns in positively selected thymocytes and their immediate
      progenitors in mice using the differential display technique. This approach lead 
      to the identification of a novel gene, mIAN-1 (murine immune-associated
      nucleotide-1), that is switched on upon positive selection and predominantly
      expressed in the lymphoid system. We show that mIAN-1 encodes a 42-kDa protein
      sharing sequence homology with the pathogen-induced plant protein aig1 and that
      it defines a novel family of at least three putative GTP-binding proteins.
      Analysis of protein expression at various stages of thymocyte development links
      mIAN-1 to CD3-mediated selection events, suggesting that it represents a key
      player of thymocyte development and that it participates to peripheral specific
      immune responses. The evolutionary conservation of the IAN family provides a
      unique example of a plant pathogen response gene conserved in animals.
FAU - Poirier, G M
AU  - Poirier GM
AD  - Anatomy Department, Medical School, Birmingham University, Edgbaston, United
      Kingdom. poirigmc@novell5.bham.ac.uk
FAU - Anderson, G
AU  - Anderson G
FAU - Huvar, A
AU  - Huvar A
FAU - Wagaman, P C
AU  - Wagaman PC
FAU - Shuttleworth, J
AU  - Shuttleworth J
FAU - Jenkinson, E
AU  - Jenkinson E
FAU - Jackson, M R
AU  - Jackson MR
FAU - Peterson, P A
AU  - Peterson PA
FAU - Erlander, M G
AU  - Erlander MG
LA  - eng
SI  - GENBANK/AA197670
GR  - Wellcome Trust/United Kingdom
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - J Immunol
JT  - Journal of immunology (Baltimore, Md. : 1950)
JID - 2985117R
RN  - 0 (Apoptosis Regulatory Proteins)
RN  - 0 (Biomarkers)
RN  - 0 (CD3 Complex)
RN  - 0 (DNA, Complementary)
RN  - 0 (Ian1 protein, mouse)
RN  - 0 (Ian3 protein, mouse)
RN  - 0 (Plant Proteins)
RN  - 0 (RNA, Messenger)
RN  - EC 3.6.1.- (GTP-Binding Proteins)
SB  - AIM
SB  - IM
MH  - Amino Acid Sequence
MH  - Animals
MH  - Apoptosis Regulatory Proteins
MH  - Base Sequence
MH  - Biomarkers/chemistry
MH  - CD3 Complex/physiology
MH  - Cell Differentiation/immunology
MH  - Cloning, Molecular
MH  - *Conserved Sequence
MH  - DNA, Complementary/chemistry/isolation & purification
MH  - GTP-Binding Proteins/*chemistry/*genetics
MH  - *Genes, Plant
MH  - Lymphocytes/metabolism
MH  - Mice
MH  - Mice, Inbred BALB C
MH  - Mice, Inbred C57BL
MH  - Mice, Knockout
MH  - Mice, Transgenic
MH  - Molecular Sequence Data
MH  - Multigene Family/*immunology
MH  - Organ Culture Techniques
MH  - Organ Specificity/genetics
MH  - Plant Proteins/genetics
MH  - RNA, Messenger/biosynthesis
MH  - Sequence Homology, Amino Acid
MH  - Signal Transduction/genetics/immunology
MH  - Thymus Gland/chemistry/*cytology/*immunology/metabolism
EDAT- 1999/10/21 00:00
MHDA- 1999/10/21 00:01
CRDT- 1999/10/21 00:00
PHST- 1999/10/21 00:00 [pubmed]
PHST- 1999/10/21 00:01 [medline]
PHST- 1999/10/21 00:00 [entrez]
AID - ji_v163n9p4960 [pii]
PST - ppublish
SO  - J Immunol. 1999 Nov 1;163(9):4960-9.