PMID- 10527952
OWN - NLM
STAT- MEDLINE
DCOM- 20000103
LR  - 20181113
IS  - 0264-6021 (Print)
IS  - 0264-6021 (Linking)
VI  - 343 Pt 3
DP  - 1999 Nov 1
TI  - Regulation of intestinal Na+-dependent phosphate co-transporters by a
      low-phosphate diet and 1,25-dihydroxyvitamin D3.
PG  - 705-12
AB  - In a study of the rat intestinal P(i) transport system, an activator protein for 
      rat Na/P(i) co-transport system (PiUS) was isolated and characterized. We also
      investigated the effects of restriction of vitamin D and P(i) (two of the most
      important physiological and pathophysiological regulators of P(i) absorption in
      the small intestine) on intestinal P(i) transport activity and the expression of 
      Na/P(i) co-transporters that are expressed in rat small intestine. Rat PiUS
      encodes a 424-residue protein with a calculated molecular mass of 51463 Da. The
      microinjection of rat PiUS into Xenopus oocytes markedly stimulated
      Na(+)-dependent P(i) co-transport activity. In rats fed with a low-P(i) diet,
      Na(+)-dependent P(i) co-transport activity was increased approx. 2-fold compared 
      with that of rats fed a normal P(i) diet. Kinetic studies demonstrated that this 
      increased activity was due to an elevation of V(max) but not K(m). The PiUS mRNA 
      levels showed an approximate doubling in the rats fed with the low-P(i) diet
      compared with those fed with the normal P(i) diet. In addition, after the
      administration of 1, 25-dihydroxyvitamin D(3) [1,25-(OH)(2)D(3)] to vitamin
      D-deficient animals, the P(i) uptake was significantly increased in the
      Na(+)-dependent component in the brush border membrane vesicle (BBMV) at 24 and
      48 h. In addition, we found a further high-affinity Na/P(i) co-transport system
      in the BBMV isolated from the vitamin D-replete animals. The levels of type III
      Na/P(i) co-transporter PiT-2 mRNA were increased 24 and 48 h after
      1,25-(OH)(2)D(3) administration to vitamin D-deficient animals, whereas PiUS and 
      the type IIb Na/P(i) co-transporter mRNA levels were unchanged. In conclusion, we
      first cloned a rat activator protein, PiUS, and then studied its role along with 
      that of other type III Na/P(i) co-transporters. PiUS and PiT-2 might be important
      components in the regulation of the intestinal P(i) transport system by P(i)
      restriction and 1,25-(OH)(2)D(3).
FAU - Katai, K
AU  - Katai K
AD  - Department of Clinical Nutrition, School of Medicine, Tokushima University,
      Kuramoto-Cho 3, Tokushima City, Tokushima 770, Japan.
FAU - Miyamoto, K
AU  - Miyamoto K
FAU - Kishida, S
AU  - Kishida S
FAU - Segawa, H
AU  - Segawa H
FAU - Nii, T
AU  - Nii T
FAU - Tanaka, H
AU  - Tanaka H
FAU - Tani, Y
AU  - Tani Y
FAU - Arai, H
AU  - Arai H
FAU - Tatsumi, S
AU  - Tatsumi S
FAU - Morita, K
AU  - Morita K
FAU - Taketani, Y
AU  - Taketani Y
FAU - Takeda, E
AU  - Takeda E
LA  - eng
SI  - GENBANK/AB015723
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - Biochem J
JT  - The Biochemical journal
JID - 2984726R
RN  - 0 (Carrier Proteins)
RN  - 0 (Phosphates)
RN  - 0 (Phosphorus, Dietary)
RN  - 0 (Recombinant Proteins)
RN  - 0 (Slc34a2 protein, rat)
RN  - 0 (Sodium-Phosphate Cotransporter Proteins)
RN  - 0 (Sodium-Phosphate Cotransporter Proteins, Type IIb)
RN  - 0 (Symporters)
RN  - FXC9231JVH (Calcitriol)
SB  - IM
MH  - Amino Acid Sequence
MH  - Animals
MH  - Base Sequence
MH  - Calcitriol/*pharmacology
MH  - Carrier Proteins/chemistry/*genetics/*metabolism
MH  - Female
MH  - Gene Expression Regulation/drug effects/*physiology
MH  - Intestinal Mucosa/*metabolism
MH  - Jejunum/*physiology
MH  - Kinetics
MH  - Male
MH  - Microvilli/drug effects/metabolism
MH  - Molecular Sequence Data
MH  - Oocytes/physiology
MH  - Phosphates/administration & dosage/*metabolism/*pharmacology
MH  - Phosphorus, Dietary/*pharmacology
MH  - Rats
MH  - Rats, Wistar
MH  - Recombinant Proteins/metabolism
MH  - Sodium-Phosphate Cotransporter Proteins
MH  - Sodium-Phosphate Cotransporter Proteins, Type IIb
MH  - *Symporters
MH  - *Transcription, Genetic/drug effects
MH  - Vitamin D Deficiency/metabolism
MH  - Xenopus laevis
PMC - PMC1220605
EDAT- 1999/10/21 00:00
MHDA- 1999/10/21 00:01
CRDT- 1999/10/21 00:00
PHST- 1999/10/21 00:00 [pubmed]
PHST- 1999/10/21 00:01 [medline]
PHST- 1999/10/21 00:00 [entrez]
PST - ppublish
SO  - Biochem J. 1999 Nov 1;343 Pt 3:705-12.