PMID- 10527948
OWN - NLM
STAT- MEDLINE
DCOM- 20000103
LR  - 20181113
IS  - 0264-6021 (Print)
IS  - 0264-6021 (Linking)
VI  - 343 Pt 3
DP  - 1999 Nov 1
TI  - Evidence for an interaction of the metalloprotease-disintegrin tumour necrosis
      factor alpha convertase (TACE) with mitotic arrest deficient 2 (MAD2), and of the
      metalloprotease-disintegrin MDC9 with a novel MAD2-related protein, MAD2beta.
PG  - 673-80
AB  - Metalloprotease-disintegrins are a family of transmembrane glycoproteins that
      have a role in fertilization, sperm migration, myoblast fusion, neural
      development and ectodomain shedding. In the present study we used the yeast
      two-hybrid system to search for proteins that interact with the cytoplasmic
      domain of two metalloprotease-disintegrins, tumour necrosis factor alpha
      convertase (TACE; ADAM17) and MDC9 (ADAM9; meltrin gamma). We have identified
      mitotic arrest deficient 2 (MAD2) as a binding partner of the TACE cytoplasmic
      domain, and a novel MAD2-related protein, MAD2beta, as a binding partner of the
      MDC9 cytoplasmic domain. MAD2beta has 23% sequence identity with MAD2, which is a
      component of the spindle assembly (or mitotic) checkpoint mechanism. Northern
      blot analysis of human tissues indicates that MAD2beta mRNA is expressed
      ubiquitously. The interaction of the TACE and MDC9 cytoplasmic domains with their
      binding partners has been confirmed biochemically. The independent identification
      of MAD2 and MAD2beta as potential interacting partners of distinct
      metalloprotease-disintegrins raises the possibility of a link between
      metalloprotease-disintegrins and the cell cycle, or of functions for MAD2 and
      MAD2beta that are not related to cell cycle control.
FAU - Nelson, K K
AU  - Nelson KK
AD  - Cellular Biochemistry and Biophysics Program, Sloan-Kettering Institute, Memorial
      Sloan-Kettering Cancer Center, New York, NY 10021, USA.
FAU - Schlondorff, J
AU  - Schlondorff J
FAU - Blobel, C P
AU  - Blobel CP
LA  - eng
GR  - 5F32GM07739-17/GM/NIGMS NIH HHS/United States
GR  - P30-CA-08748/CA/NCI NIH HHS/United States
GR  - R55GM51988/GM/NIGMS NIH HHS/United States
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - England
TA  - Biochem J
JT  - The Biochemical journal
JID - 2984726R
RN  - 0 (Calcium-Binding Proteins)
RN  - 0 (Carrier Proteins)
RN  - 0 (Cell Cycle Proteins)
RN  - 0 (DNA-Binding Proteins)
RN  - 0 (Disintegrins)
RN  - 0 (Fungal Proteins)
RN  - 0 (MAD2 protein, S cerevisiae)
RN  - 0 (Mad2 Proteins)
RN  - 0 (Membrane Proteins)
RN  - 0 (Nuclear Proteins)
RN  - 0 (Recombinant Proteins)
RN  - 0 (SMAD2 protein, human)
RN  - 0 (Saccharomyces cerevisiae Proteins)
RN  - 0 (Smad2 Protein)
RN  - 0 (Trans-Activators)
RN  - 0 (Tumor Necrosis Factor-alpha)
RN  - EC 3.4.24.- (ADAM Proteins)
RN  - EC 3.4.24.- (ADAM9 protein, human)
RN  - EC 3.4.24.- (Metalloendopeptidases)
RN  - EC 3.4.24.86 (ADAM17 Protein)
RN  - EC 3.4.24.86 (ADAM17 protein, human)
SB  - IM
MH  - ADAM Proteins
MH  - ADAM17 Protein
MH  - Amino Acid Sequence
MH  - Animals
MH  - COS Cells
MH  - Calcium-Binding Proteins/chemistry/isolation & purification/*metabolism
MH  - *Carrier Proteins
MH  - Cell Cycle Proteins
MH  - Cloning, Molecular
MH  - DNA-Binding Proteins/chemistry/isolation & purification/*metabolism
MH  - Disintegrins/chemistry/isolation & purification/*metabolism
MH  - Fungal Proteins/chemistry/isolation & purification/*metabolism
MH  - Humans
MH  - Mad2 Proteins
MH  - Membrane Proteins/metabolism
MH  - Metalloendopeptidases/chemistry/isolation & purification/*metabolism
MH  - Molecular Sequence Data
MH  - Nuclear Proteins
MH  - Recombinant Proteins/chemistry/isolation & purification/metabolism
MH  - Saccharomyces cerevisiae/metabolism
MH  - Saccharomyces cerevisiae Proteins
MH  - Sequence Alignment
MH  - Sequence Homology, Amino Acid
MH  - Smad2 Protein
MH  - Trans-Activators/chemistry/isolation & purification/*metabolism
MH  - Transfection
MH  - Tumor Necrosis Factor-alpha/metabolism
PMC - PMC1220601
EDAT- 1999/10/21 00:00
MHDA- 1999/10/21 00:01
CRDT- 1999/10/21 00:00
PHST- 1999/10/21 00:00 [pubmed]
PHST- 1999/10/21 00:01 [medline]
PHST- 1999/10/21 00:00 [entrez]
PST - ppublish
SO  - Biochem J. 1999 Nov 1;343 Pt 3:673-80.