PMID- 10527891
OWN - NLM
STAT- MEDLINE
DCOM- 20000906
LR  - 20071114
IS  - 1522-4724 (Print)
IS  - 1522-4724 (Linking)
VI  - 2
IP  - 1
DP  - 1999 Jul
TI  - The human thrombospondin 3 gene: analysis of transcription initiation and an
      alternatively spliced transcript.
PG  - 47-52
AB  - Thrombospondin 3 (TSP3) is a member of a family of modular, extracellular
      proteins that have been implicated in a diverse number of important biological
      processes. To contribute to an understanding of the precise roles of human TSP3, 
      aspects of TSP3 gene transcription have been investigated. The TSP3 gene (THBS3) 
      shares a promoter/intergenic region of 1.4 kb with the divergently transcribed
      metaxin gene, and the existence of TSP3 transcription initiation sites in the
      TSP3/metaxin intergenic region was investigated by a PCR procedure. Transcripts
      were detected which initiate in the intergenic region, up to several hundred
      bases upstream from the major transcription start site. An alternatively spliced 
      transcript of TSP3 was also detected by the PCR procedure. This includes a new
      exon, exon A', which replaces exon A. Exon A' is located in the TSP3/metaxin
      intergenic region, 1 kb 5' of exon A. In addition, transcripts of metaxin were
      found with extended 5' ends; these overlap the 5' end of the TSP3 alternative
      transcript. The complexities of TSP3 transcription initiation revealed by this
      study could contribute to the tissue-specific expression and diverse functions of
      TSP3.
FAU - Adolph, K W
AU  - Adolph KW
AD  - Department of Biochemistry, Molecular Biology and Biophysics, University of
      Minnesota, Minneapolis 55455, USA. ka@brain.biochem.umn.edu
FAU - Bornstein, P
AU  - Bornstein P
LA  - eng
GR  - DE08229/DE/NIDCR NIH HHS/United States
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - Mol Cell Biol Res Commun
JT  - Molecular cell biology research communications : MCBRC
JID - 100889076
RN  - 0 (MTX1 protein, human)
RN  - 0 (Proteins)
RN  - 0 (RNA, Messenger)
RN  - 0 (Thrombospondins)
RN  - 0 (thrombospondin 3)
RN  - 9007-49-2 (DNA)
SB  - IM
MH  - *Alternative Splicing
MH  - Amino Acid Sequence
MH  - Base Sequence
MH  - DNA/analysis
MH  - Exons
MH  - Gene Expression Regulation
MH  - Humans
MH  - Introns
MH  - Molecular Sequence Data
MH  - Organ Specificity
MH  - Proteins/genetics/*metabolism
MH  - RNA, Messenger/analysis
MH  - Regulatory Sequences, Nucleic Acid/*genetics
MH  - Sequence Analysis, DNA
MH  - Thrombospondins/genetics/*metabolism
MH  - Transcription, Genetic
EDAT- 1999/11/30 09:00
MHDA- 2000/09/09 11:01
CRDT- 1999/11/30 09:00
PHST- 1999/11/30 09:00 [pubmed]
PHST- 2000/09/09 11:01 [medline]
PHST- 1999/11/30 09:00 [entrez]
AID - 10.1006/mcbr.1999.0148 [doi]
AID - S1522472499901484 [pii]
PST - ppublish
SO  - Mol Cell Biol Res Commun. 1999 Jul;2(1):47-52. doi: 10.1006/mcbr.1999.0148.