PMID- 10527559
OWN - NLM
STAT- MEDLINE
DCOM- 19991117
LR  - 20071114
IS  - 0008-8749 (Print)
IS  - 0008-8749 (Linking)
VI  - 196
IP  - 2
DP  - 1999 Sep 15
TI  - Cloning of a novel MHC-encoded serine peptidase highly expressed by cortical
      epithelial cells of the thymus.
PG  - 80-6
AB  - Antigen presentation by cortical thymic epithelial cells (cTEC) during the
      positive selection of T cells has been shown to differ from that of other
      antigen-presenting cells. In the case of MHC class II presentation, cathepsin L
      as opposed to cathepsin S is responsible at least in part for the degradation of 
      invariant chain. Other proteases, however, must be involved. We have identified a
      putative serine protease that is specifically expressed in the thymus. Encoded
      within the class I major histocompatibility complex (MHC) region, this gene has
      sequence homology with lysosomal prolylcarboxypeptidase, suggesting that it is a 
      serine protease. We have, therefore, designated this gene thymus-specific serine 
      protease (TSSP). In situ hybridization and immunofluorescence staining reveal
      that TSSP is expressed exclusively by cortical thymic epithelial cells, with the 
      strongest staining noted around vessels and the thymic capsule. The
      identification of TSSP further supports the theory that MHC class II antigen
      processing and presentation in the thymic cortex involves a proteolytic milieu
      that differs from that of other antigen-presenting cells.
CI  - Copyright 1999 Academic Press.
FAU - Bowlus, C L
AU  - Bowlus CL
AD  - Department of Internal Medicine, University of California Davis Medical Center,
      Sacramento, California 95817, USA. clbowlus@ucdavis.edu
FAU - Ahn, J
AU  - Ahn J
FAU - Chu, T
AU  - Chu T
FAU - Gruen, J R
AU  - Gruen JR
LA  - eng
SI  - GENBANK/AF052514
GR  - DK02398/DK/NIDDK NIH HHS/United States
GR  - DK45819/DK/NIDDK NIH HHS/United States
PT  - Comparative Study
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - Netherlands
TA  - Cell Immunol
JT  - Cellular immunology
JID - 1246405
RN  - 0 (Antigens, Differentiation, B-Lymphocyte)
RN  - 0 (Histocompatibility Antigens Class II)
RN  - 0 (invariant chain)
RN  - EC 3.4.- (Carboxypeptidases)
RN  - EC 3.4.16.2 (lysosomal Pro-X carboxypeptidase)
RN  - EC 3.4.21.- (Serine Endopeptidases)
SB  - IM
MH  - Amino Acid Sequence
MH  - Animals
MH  - Antigen Presentation
MH  - Antigens, Differentiation, B-Lymphocyte/metabolism
MH  - Base Sequence
MH  - Carboxypeptidases/chemistry
MH  - Consensus Sequence
MH  - Cosmids
MH  - Epithelial Cells/enzymology
MH  - Fluorescent Antibody Technique, Indirect
MH  - *Genes
MH  - Histocompatibility Antigens Class II/metabolism
MH  - Humans
MH  - In Situ Hybridization
MH  - Lysosomes/enzymology
MH  - Major Histocompatibility Complex/*genetics
MH  - Mice
MH  - Mice, Inbred C57BL
MH  - Molecular Sequence Data
MH  - Sequence Alignment
MH  - Sequence Homology, Amino Acid
MH  - Serine Endopeptidases/*genetics/isolation & purification
MH  - Thymus Gland/*enzymology/growth & development
EDAT- 1999/10/21 00:00
MHDA- 1999/10/21 00:01
CRDT- 1999/10/21 00:00
PHST- 1999/10/21 00:00 [pubmed]
PHST- 1999/10/21 00:01 [medline]
PHST- 1999/10/21 00:00 [entrez]
AID - 10.1006/cimm.1999.1543 [doi]
AID - S0008-8749(99)91543-5 [pii]
PST - ppublish
SO  - Cell Immunol. 1999 Sep 15;196(2):80-6. doi: 10.1006/cimm.1999.1543.