PMID- 10527525
OWN - NLM
STAT- MEDLINE
DCOM- 19991213
LR  - 20061115
IS  - 0003-2697 (Print)
IS  - 0003-2697 (Linking)
VI  - 274
IP  - 2
DP  - 1999 Oct 15
TI  - Alkaline density gradient floatation of membranes: polypeptide composition of the
      mammalian peroxisomal membrane.
PG  - 270-7
AB  - A method for purification of the peroxisomal membrane from rat liver is
      described. The procedure consists of floating the (contaminated) peroxisomal
      membranes through an alkaline sucrose density gradient. A good resolution between
      the peroxisomal membrane and other membrane systems is achieved. Using these
      floated peroxisomal membranes we have determined that only 7.8 +/- 0.9% of the
      total peroxisomal protein is alkali resistant. The polypeptide composition of
      these highly pure peroxisomal membranes was analyzed by SDS-PAGE. Bands
      corresponding to polypeptides with apparent molecular masses of 15, 18, 22, 24,
      26, 29, 35, 36, 38, 40, 52, 55, 70, 74-77, and 88 kDa are detected upon Coomassie
      blue staining of polyacrylamide gels. The identity of several of these
      polypeptides was determined by N-terminal sequencing and Western blotting
      analysis.
CI  - Copyright 1999 Academic Press.
FAU - Gouveia, A M
AU  - Gouveia AM
AD  - Instituto de Biologia Molecular e Celular, Universidade do Porto, Porto,
      Portugal.
FAU - Reguenga, C
AU  - Reguenga C
FAU - Oliveira, M E
AU  - Oliveira ME
FAU - Eckerskorn, C
AU  - Eckerskorn C
FAU - Sa-Miranda, C
AU  - Sa-Miranda C
FAU - Azevedo, J E
AU  - Azevedo JE
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Anal Biochem
JT  - Analytical biochemistry
JID - 0370535
RN  - 0 (Peptides)
SB  - IM
MH  - Amino Acid Sequence
MH  - Animals
MH  - Cell Fractionation/methods
MH  - Centrifugation, Density Gradient/*methods
MH  - Electrophoresis, Polyacrylamide Gel
MH  - Intracellular Membranes/*chemistry
MH  - Male
MH  - Molecular Sequence Data
MH  - Peptides/*chemistry
MH  - Peroxisomes/*chemistry
MH  - Rats
MH  - Rats, Wistar
EDAT- 1999/10/21 00:00
MHDA- 1999/10/21 00:01
CRDT- 1999/10/21 00:00
PHST- 1999/10/21 00:00 [pubmed]
PHST- 1999/10/21 00:01 [medline]
PHST- 1999/10/21 00:00 [entrez]
AID - 10.1006/abio.1999.4281 [doi]
AID - S0003-2697(99)94281-1 [pii]
PST - ppublish
SO  - Anal Biochem. 1999 Oct 15;274(2):270-7. doi: 10.1006/abio.1999.4281.