PMID- 10526179
OWN - NLM
STAT- MEDLINE
DCOM- 19991122
LR  - 20190621
IS  - 0014-5793 (Print)
IS  - 0014-5793 (Linking)
VI  - 459
IP  - 3
DP  - 1999 Oct 15
TI  - Cloning and characterization of two novel aldo-keto reductases (AKR1C12 and
      AKR1C13) from mouse stomach.
PG  - 433-7
AB  - In contrast to hepatic hydrosteroid dehydrogenases (HSDs) of the aldo-keto
      reductase family (AKR1C), little is known about a stomach one. From a mouse
      stomach cDNA library, we isolated two clones encoding proteins of 323 amino acid 
      residues. They exhibited 93.2% amino acid sequence identity and 64-68% with any
      known HSDs. Recombinant proteins expressed in Escherichia coli reduced
      9,10-phenanthraquinone with NAD(P)H as cofactor. The mRNAs were exclusively
      expressed in stomach, liver and ileum. The present study demonstrates that these 
      proteins are new members of the HSD subfamily and they are named AKR1C12 and
      AKR1C13. Immunohistochemical analysis suggests that they are involved in
      detoxification of xenobiotics in the stomach.
FAU - Ikeda, S
AU  - Ikeda S
AD  - Department of Anesthesiology, Kansai Medical University, Moriguchi, Japan.
FAU - Okuda-Ashitaka, E
AU  - Okuda-Ashitaka E
FAU - Masu, Y
AU  - Masu Y
FAU - Suzuki, T
AU  - Suzuki T
FAU - Watanabe, K
AU  - Watanabe K
FAU - Nakao, M
AU  - Nakao M
FAU - Shingu, K
AU  - Shingu K
FAU - Ito, S
AU  - Ito S
LA  - eng
SI  - GENBANK/AB027125
SI  - GENBANK/AB027237
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - FEBS Lett
JT  - FEBS letters
JID - 0155157
RN  - 0 (DNA, Complementary)
RN  - EC 1.1.- (Alcohol Oxidoreductases)
RN  - EC 1.1.1.- (Aldo-Keto Reductases)
RN  - EC 1.1.1.- (Hydroxyprostaglandin Dehydrogenases)
RN  - EC 1.1.1.188 (prostaglandin-F synthase)
RN  - EC 1.1.1.21 (Aldehyde Reductase)
RN  - EC 1.1.1.n11 (AKR7A5 protein, mouse)
SB  - IM
MH  - Alcohol Oxidoreductases/classification/*genetics/metabolism
MH  - Aldehyde Reductase
MH  - Aldo-Keto Reductases
MH  - Amino Acid Sequence
MH  - Animals
MH  - Cloning, Molecular
MH  - DNA, Complementary/analysis
MH  - Escherichia coli
MH  - Gastric Mucosa/metabolism
MH  - Hydroxyprostaglandin Dehydrogenases/chemistry/genetics
MH  - Mice
MH  - Molecular Sequence Data
MH  - Phylogeny
MH  - Sequence Homology, Amino Acid
MH  - Stomach/*enzymology
MH  - Tissue Distribution
EDAT- 1999/10/20 00:00
MHDA- 1999/10/20 00:01
CRDT- 1999/10/20 00:00
PHST- 1999/10/20 00:00 [pubmed]
PHST- 1999/10/20 00:01 [medline]
PHST- 1999/10/20 00:00 [entrez]
AID - S0014-5793(99)01243-0 [pii]
AID - 10.1016/s0014-5793(99)01243-0 [doi]
PST - ppublish
SO  - FEBS Lett. 1999 Oct 15;459(3):433-7. doi: 10.1016/s0014-5793(99)01243-0.