PMID- 10526176
OWN - NLM
STAT- MEDLINE
DCOM- 19991122
LR  - 20190621
IS  - 0014-5793 (Print)
IS  - 0014-5793 (Linking)
VI  - 459
IP  - 3
DP  - 1999 Oct 15
TI  - Characterization of recombinant human glucuronyltransferase I involved in the
      biosynthesis of the glycosaminoglycan-protein linkage region of proteoglycans.
PG  - 415-20
AB  - We characterized the recombinant glucuronyltransferase I (GlcAT-I) involved in
      the glycosaminoglycan-protein linkage region biosynthesis. The enzyme showed
      strict specificity for Galbeta1-3Galbeta1-4Xyl, exhibiting negligible
      incorporation into other galactoside substrates including
      Galbeta1-3Galbeta1-O-benzyl, Galbeta1-4GlcNAc and Galbeta1-4Glc. A comparison of 
      the GlcAT-I with another beta1,3-glucuronyltransferase involved in the HNK-1
      epitope biosynthesis revealed that the two beta1,3-glucuronyltransferases
      exhibited distinct and no overlapping acceptor substrate specificities in vitro. 
      Nevertheless, the transfection of the GlcAT-I cDNA into COS-1 cells induced the
      significant expression of the HNK-1 epitope. These results suggested that the
      high expression of the GlcAT-I gene rendered the cells capable of synthesizing
      the HNK-1 epitope.
FAU - Tone, Y
AU  - Tone Y
AD  - Department of Biochemistry, Kobe Pharmaceutical University, 4-19-1
      Motoyamakita-machi, Higashinada-ku, Kobe, Japan.
FAU - Kitagawa, H
AU  - Kitagawa H
FAU - Imiya, K
AU  - Imiya K
FAU - Oka, S
AU  - Oka S
FAU - Kawasaki, T
AU  - Kawasaki T
FAU - Sugahara, K
AU  - Sugahara K
LA  - eng
PT  - Comparative Study
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - FEBS Lett
JT  - FEBS letters
JID - 0155157
RN  - 0 (Glycosaminoglycans)
RN  - 0 (Proteins)
RN  - 0 (Proteoglycans)
RN  - 0 (Recombinant Proteins)
RN  - EC 2.4.1.135 (galactosylgalactoylxylosylprotein 3-beta-glucuronosyltransferase)
RN  - EC 2.4.1.17 (Glucuronosyltransferase)
SB  - IM
MH  - Animals
MH  - COS Cells
MH  - Glucuronosyltransferase/biosynthesis/genetics/*metabolism
MH  - Glycosaminoglycans/*metabolism
MH  - Humans
MH  - Proteins/metabolism
MH  - Proteoglycans/*biosynthesis/metabolism
MH  - Recombinant Proteins/metabolism
MH  - Substrate Specificity
EDAT- 1999/10/20 00:00
MHDA- 1999/10/20 00:01
CRDT- 1999/10/20 00:00
PHST- 1999/10/20 00:00 [pubmed]
PHST- 1999/10/20 00:01 [medline]
PHST- 1999/10/20 00:00 [entrez]
AID - S0014-5793(99)01287-9 [pii]
AID - 10.1016/s0014-5793(99)01287-9 [doi]
PST - ppublish
SO  - FEBS Lett. 1999 Oct 15;459(3):415-20. doi: 10.1016/s0014-5793(99)01287-9.