PMID- 10526164
OWN - NLM
STAT- MEDLINE
DCOM- 19991122
LR  - 20190621
IS  - 0014-5793 (Print)
IS  - 0014-5793 (Linking)
VI  - 459
IP  - 3
DP  - 1999 Oct 15
TI  - Kinetics of apolipoprotein E isoforms-binding to the major glycosaminoglycans of 
      the extracellular matrix.
PG  - 353-7
AB  - Apolipoprotein E (apoE), a key lipid transport protein, displays a
      heparin-binding property that is critical in several apoE functions. The kinetics
      of the interaction between apoE isoforms and glycosaminoglycans (GAGs) were
      studied using surface plasmon resonance. The dissociation constant of equilibrium
      K(D) for apoE3-heparin interaction was estimated to be 12 nM for apoE3 and three 
      common apoE isoforms revealed similar affinities for heparin. ApoE binds to GAGs 
      in the following order: heparin>heparan sulfate>dermatan sulfate>chondroitin
      sulfate. The affinity parameter of the binding of low molecular weight heparins
      to apoE is correlated with the chain length. The effective number Z of
      electrostatic interactions between plasma apoE3 and heparin was assessed to be
      three. Metal chelators were able to diminish apoE-binding to heparin, suggesting 
      some stabilizing effect of metal ions while reconstitution with lipids did not
      affect binding affinities for heparin, suggesting that the N-terminal
      heparin-binding site is responsible for apoE-containing lipoprotein interactions 
      with heparin.
FAU - Shuvaev, V V
AU  - Shuvaev VV
AD  - Centre du Medicament, Universite Henri Poincare Nancy I, 30 rue Lionnois, 54000, 
      Nancy, France.
FAU - Laffont, I
AU  - Laffont I
FAU - Siest, G
AU  - Siest G
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - FEBS Lett
JT  - FEBS letters
JID - 0155157
RN  - 0 (Apolipoproteins E)
RN  - 0 (Glycosaminoglycans)
RN  - 0 (Protein Isoforms)
RN  - 24967-94-0 (Dermatan Sulfate)
RN  - 9005-49-6 (Heparin)
RN  - 9007-28-7 (Chondroitin Sulfates)
RN  - 9050-30-0 (Heparitin Sulfate)
SB  - IM
MH  - Apolipoproteins E/*metabolism
MH  - Binding, Competitive
MH  - Biotinylation
MH  - Chondroitin Sulfates/metabolism
MH  - Dermatan Sulfate/metabolism
MH  - Extracellular Matrix/*metabolism
MH  - Glycosaminoglycans/*metabolism
MH  - Heparin/metabolism
MH  - Heparitin Sulfate/metabolism
MH  - Humans
MH  - Kinetics
MH  - Protein Isoforms/*metabolism
MH  - Surface Plasmon Resonance
EDAT- 1999/10/20 09:00
MHDA- 2000/05/20 09:00
CRDT- 1999/10/20 09:00
PHST- 1999/10/20 09:00 [pubmed]
PHST- 2000/05/20 09:00 [medline]
PHST- 1999/10/20 09:00 [entrez]
AID - S0014-5793(99)01285-5 [pii]
AID - 10.1016/s0014-5793(99)01285-5 [doi]
PST - ppublish
SO  - FEBS Lett. 1999 Oct 15;459(3):353-7. doi: 10.1016/s0014-5793(99)01285-5.