PMID- 10526156
OWN - NLM
STAT- MEDLINE
DCOM- 19991122
LR  - 20190621
IS  - 0014-5793 (Print)
IS  - 0014-5793 (Linking)
VI  - 459
IP  - 3
DP  - 1999 Oct 15
TI  - Rho-specific binding and guanine nucleotide exchange catalysis by KIAA0380, a dbl
      family member.
PG  - 313-8
AB  - Several guanine nucleotide exchange factors (GEFs) for Rho-GTPases have been
      identified, all of them containing a Dbl homology (DH) and pleckstrin homology
      (PH) domain, but exhibiting different specificities to the Rho family members,
      Rho, Rac and Cdc42. We report here that KIAA0380, a protein with a tandem DH/PH
      domain, an amino-terminal PDZ domain and a regulator of G protein signalling
      (RGS) homology domain, is a specific GEF for RhoA, but not for Rac1 and Cdc42, as
      determined by GDP release, guanosine 5'-O-(3-thio)triphosphate (GTPgammaS)
      binding and protein binding assays. When expressed in J82 cells, DH/PH
      domain-containing forms of KIAA0380 induced actin stress fibers, whereas
      expression of the RGS homology domain prevented lysophosphatidic acid
      (LPA)-induced stress fiber formation.
FAU - Rumenapp, U
AU  - Rumenapp U
AD  - Institut fur Pharmakologie, Universitatsklinikum Essen, Hufelandstr. 55, D-45122,
      Essen, Germany. ulrich.ruemenapp@uni-essen.de
FAU - Blomquist, A
AU  - Blomquist A
FAU - Schworer, G
AU  - Schworer G
FAU - Schablowski, H
AU  - Schablowski H
FAU - Psoma, A
AU  - Psoma A
FAU - Jakobs, K H
AU  - Jakobs KH
LA  - eng
SI  - GENBANK/AB002378
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - FEBS Lett
JT  - FEBS letters
JID - 0155157
RN  - 0 (ARHGEF11 protein, human)
RN  - 0 (Actins)
RN  - 0 (Guanine Nucleotide Exchange Factors)
RN  - 0 (Guanine Nucleotides)
RN  - 0 (MCF2 protein, human)
RN  - 0 (Oligopeptides)
RN  - 0 (Proto-Oncogene Proteins)
RN  - 0 (Receptors, Cell Surface)
RN  - 0 (Receptors, G-Protein-Coupled)
RN  - 0 (Receptors, Lysophosphatidic Acid)
RN  - 0 (Rho Guanine Nucleotide Exchange Factors)
RN  - 78VO7F77PN (arginyl-glycyl-aspartic acid)
RN  - EC 3.6.5.2 (rhoA GTP-Binding Protein)
SB  - IM
EIN - FEBS Lett 2000 Feb 4;467(1):134-5
MH  - Actins/metabolism
MH  - Catalysis
MH  - Guanine Nucleotide Exchange Factors/chemistry/classification/*metabolism
MH  - Guanine Nucleotides/*metabolism
MH  - Humans
MH  - Molecular Sequence Data
MH  - Oligopeptides/chemistry/metabolism
MH  - Protein Conformation
MH  - Proto-Oncogene Proteins/chemistry/classification
MH  - Receptors, Cell Surface/metabolism
MH  - *Receptors, G-Protein-Coupled
MH  - Receptors, Lysophosphatidic Acid
MH  - Rho Guanine Nucleotide Exchange Factors
MH  - Tumor Cells, Cultured
MH  - rhoA GTP-Binding Protein/*metabolism
EDAT- 1999/10/20 09:00
MHDA- 2000/03/11 09:00
CRDT- 1999/10/20 09:00
PHST- 1999/10/20 09:00 [pubmed]
PHST- 2000/03/11 09:00 [medline]
PHST- 1999/10/20 09:00 [entrez]
AID - S0014-5793(99)01270-3 [pii]
AID - 10.1016/s0014-5793(99)01270-3 [doi]
PST - ppublish
SO  - FEBS Lett. 1999 Oct 15;459(3):313-8. doi: 10.1016/s0014-5793(99)01270-3.