PMID- 10525409
OWN - NLM
STAT- MEDLINE
DCOM- 19991104
LR  - 20131121
IS  - 0022-2836 (Print)
IS  - 0022-2836 (Linking)
VI  - 292
IP  - 4
DP  - 1999 Oct 1
TI  - Structure of recombinant mouse collagenase-3 (MMP-13).
PG  - 837-44
AB  - The matrix metalloproteinases are crucial in the physiological and pathological
      degradation of the mammalian extracellular matrix, including breast tumours, and 
      osteoarthritic cartilage. These enzymes are classified according to their matrix 
      substrate specificity. Collagenase-3 (MMP-13) is a member of this family and
      preferentially cleaves type II collagen, cartilage, fibronectin and aggrecan.
      Collagenase-3 is normally expressed in hypertrophic chondrocytes, periosteal
      cells, and osteoblasts during bone development. The structure of the catalytic
      domain of recombinant mouse collagenase-3, complexed to the hydroxamate inhibitor
      (RS-113456), is reported at 2.0 A resolution. Molecular replacement and weak
      phasing information from a single derivative determined the structure. Neither
      molecular replacement nor derivative methods had a sufficient radius of
      convergence to yield a refinable structure. The structure illuminates the atomic 
      zinc ion interactions with functional groups in the active site, emphasizing zinc
      ligation and the very voluminous hydrophobic P1' group for the inhibitor potency.
      The structure provides insight into the specificity of this enzyme, facilitating 
      design of specific inhibitors to target various diseases.
FAU - Botos, I
AU  - Botos I
AD  - Department of Biochemistry and Biophysics, Texas A&M University, TX, 77843-2128, 
      USA.
FAU - Meyer, E
AU  - Meyer E
FAU - Swanson, S M
AU  - Swanson SM
FAU - Lemaitre, V
AU  - Lemaitre V
FAU - Eeckhout, Y
AU  - Eeckhout Y
FAU - Meyer, E F
AU  - Meyer EF
LA  - eng
SI  - PDB/1CXV
SI  - SWISSPROT/P23097
SI  - SWISSPROT/P33435
SI  - SWISSPROT/P45452
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Netherlands
TA  - J Mol Biol
JT  - Journal of molecular biology
JID - 2985088R
RN  - 0 (Hydroxamic Acids)
RN  - 0 (Matrix Metalloproteinase Inhibitors)
RN  - 0 (Pyrans)
RN  - 0 (RS 113456)
RN  - 0 (Recombinant Proteins)
RN  - EC 3.4.24.- (Collagenases)
RN  - EC 3.4.24.- (MMP13 protein, human)
RN  - EC 3.4.24.- (Matrix Metalloproteinase 13)
RN  - EC 3.4.24.- (Matrix Metalloproteinases)
RN  - EC 3.4.24.- (Mmp13 protein, mouse)
RN  - J41CSQ7QDS (Zinc)
RN  - SY7Q814VUP (Calcium)
SB  - IM
MH  - Amino Acid Sequence
MH  - Animals
MH  - Binding Sites
MH  - Calcium/metabolism
MH  - *Catalytic Domain
MH  - Collagenases/*chemistry/*metabolism
MH  - Crystallization
MH  - Crystallography, X-Ray
MH  - Humans
MH  - Hydrogen Bonding
MH  - Hydroxamic Acids/chemistry/metabolism
MH  - Matrix Metalloproteinase 13
MH  - Matrix Metalloproteinase Inhibitors
MH  - Matrix Metalloproteinases/*chemistry/*metabolism
MH  - Mice
MH  - Models, Molecular
MH  - Molecular Sequence Data
MH  - Protein Binding
MH  - Protein Conformation
MH  - Pyrans/chemistry/*metabolism
MH  - Recombinant Proteins/antagonists & inhibitors/chemistry/metabolism
MH  - Zinc/metabolism
EDAT- 1999/10/20 00:00
MHDA- 1999/10/20 00:01
CRDT- 1999/10/20 00:00
PHST- 1999/10/20 00:00 [pubmed]
PHST- 1999/10/20 00:01 [medline]
PHST- 1999/10/20 00:00 [entrez]
AID - 10.1006/jmbi.1999.3068 [doi]
AID - S0022-2836(99)93068-1 [pii]
PST - ppublish
SO  - J Mol Biol. 1999 Oct 1;292(4):837-44. doi: 10.1006/jmbi.1999.3068.