PMID- 10525162
OWN - NLM
STAT- MEDLINE
DCOM- 19991123
LR  - 20190610
IS  - 0006-3002 (Print)
IS  - 0006-3002 (Linking)
VI  - 1452
IP  - 1
DP  - 1999 Oct 13
TI  - Identification of soluble interleukin-4 receptor in rat glomerular epithelial
      cells(1).
PG  - 79-88
AB  - Interleukin (IL)-4, a pleiotropic cytokine involved in many glomerular diseases, 
      is regulated positively by membrane-bound IL-4R (mIL-4R) and negatively by
      soluble IL-4R (sIL-4R). Because natural sIL-4R has been documented only in mice, 
      we undertook this study in rats to determine whether they, too, express sIL-4R,
      particularly in kidney cells. A pair of IL-4R primers was designed for this
      purpose and used in the polymerase chain reaction. As a result, sIL-4R was found 
      not only in rats spleen cells but also in their glomerular epithelial cells
      (GEC). Sequence analysis revealed that the mRNA of rat sIL-4R has a 75-bp insert 
      sequence. This insert generated a termination TGA codon upstream from the
      transmembrane region, resulting in formation of the sIL-4R. Subsequent screening 
      of the kidney cDNA library enabled us to obtain the whole 3605-bp cDNA of sIL-4R;
      the full-length 3530-bp mIL-4R cDNA was also identified as a much longer sequence
      than previously published. Among the total 39 clones positive for IL-4R, two were
      confirmed as sIL-4R, and 37 clones were positive for mIL-4R. Next, the translated
      portion of sIL-4R cDNA was constructed into an expression vector, enabling us to 
      obtain a recombinant sIL4R-myc fusion protein. By using this recombinant sIL-4R, 
      we proved that sIL-4R can antagonize the IL-4-induced proliferation of spleen
      cells. Present study demonstrated that sIL-4R is expressed in kidney cells and
      antagonistically functional.
FAU - Chen, G
AU  - Chen G
AD  - Department of Molecular Pathology, Tokyo Metropolitan Institute of Gerontology,
      35-2 Sakaecho, Itabashi-ku, Tokyo, Japan.
FAU - Nagasawa, R
AU  - Nagasawa R
FAU - Imasawa, T
AU  - Imasawa T
FAU - Eto, Y
AU  - Eto Y
FAU - Kikuchi, K
AU  - Kikuchi K
FAU - Maruyama, N
AU  - Maruyama N
LA  - eng
SI  - GENBANK/AB015746
SI  - GENBANK/AB015747
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Netherlands
TA  - Biochim Biophys Acta
JT  - Biochimica et biophysica acta
JID - 0217513
RN  - 0 (DNA, Complementary)
RN  - 0 (Receptors, Interleukin-4)
RN  - 0 (Recombinant Fusion Proteins)
RN  - 207137-56-2 (Interleukin-4)
SB  - IM
MH  - Animals
MH  - Base Sequence
MH  - COS Cells
MH  - Cell Division/drug effects
MH  - Cell Membrane/metabolism
MH  - Cells, Cultured
MH  - DNA, Complementary/isolation & purification
MH  - Epithelial Cells/*metabolism
MH  - Gene Library
MH  - Interleukin-4/antagonists & inhibitors/pharmacology
MH  - Kidney Glomerulus/*metabolism
MH  - Male
MH  - Molecular Sequence Data
MH  - Rats
MH  - Rats, Wistar
MH  - Receptors, Interleukin-4/*analysis/genetics
MH  - Recombinant Fusion Proteins/isolation & purification/metabolism
MH  - Reverse Transcriptase Polymerase Chain Reaction
MH  - Solubility
MH  - Spleen
EDAT- 1999/10/19 00:00
MHDA- 1999/10/19 00:01
CRDT- 1999/10/19 00:00
PHST- 1999/10/19 00:00 [pubmed]
PHST- 1999/10/19 00:01 [medline]
PHST- 1999/10/19 00:00 [entrez]
AID - S0167488999001172 [pii]
AID - 10.1016/s0167-4889(99)00117-2 [doi]
PST - ppublish
SO  - Biochim Biophys Acta. 1999 Oct 13;1452(1):79-88. doi:
      10.1016/s0167-4889(99)00117-2.