PMID- 10525161
OWN - NLM
STAT- MEDLINE
DCOM- 19991123
LR  - 20190610
IS  - 0006-3002 (Print)
IS  - 0006-3002 (Linking)
VI  - 1452
IP  - 1
DP  - 1999 Oct 13
TI  - Each mammalian mitochondrial outer membrane porin protein is dispensable: effects
      on cellular respiration.
PG  - 68-78
AB  - Voltage-dependent anion channels (VDACs, also known as mitochondrial porins) are 
      small pore-forming proteins of the mitochondrial outer membrane found in all
      eukaryotes. Mammals harbor three distinct VDAC isoforms, with each protein
      sharing 65-70% sequence identity. Deletion of the yeast VDAC1 gene leads to
      conditional lethality that can be partially or completely complemented by the
      mammalian VDAC genes. In vitro, VDACs conduct a variety of small metabolites and 
      in vivo they serve as a binding site for several cytosolic kinases involved in
      intermediary metabolism, yet the specific physiologic role of each isoform is
      unknown. Here we show that mouse embryonic stem cells lacking each isoform are
      viable but exhibit a 30% reduction in oxygen consumption. VDAC1 and VDAC2
      deficient cells exhibit reduced cytochrome c oxidase activity, whereas VDAC3
      deficient cells have normal activity. These results indicate that VDACs are not
      essential for cell viability and we speculate that reduced respiration in part
      reflects decreased outer membrane permeability for small metabolites necessary
      for oxidative phosphorylation.
FAU - Wu, S
AU  - Wu S
AD  - Department of Molecular and Human Genetics, Baylor College of Medicine, One
      Baylor Plaza, Houston, TX 77030, USA.
FAU - Sampson, M J
AU  - Sampson MJ
FAU - Decker, W K
AU  - Decker WK
FAU - Craigen, W J
AU  - Craigen WJ
LA  - eng
GR  - 1P30-HD27823/HD/NICHD NIH HHS/United States
GR  - R01 GM055713-02/GM/NIGMS NIH HHS/United States
PT  - Journal Article
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - Netherlands
TA  - Biochim Biophys Acta
JT  - Biochimica et biophysica acta
JID - 0217513
RN  - 0 (Ion Channels)
RN  - 0 (Porins)
RN  - 0 (Vdac1 protein, mouse)
RN  - 0 (Vdac2 protein, mouse)
RN  - 0 (Voltage-Dependent Anion Channel 2)
RN  - 0 (Voltage-Dependent Anion Channels)
RN  - 63231-63-0 (RNA)
RN  - EC 1.6.- (Voltage-Dependent Anion Channel 1)
RN  - EC 1.9.3.1 (Electron Transport Complex IV)
SB  - IM
MH  - Animals
MH  - Blotting, Western
MH  - Cell Line
MH  - Cell Survival
MH  - Electron Transport Complex IV/metabolism
MH  - Gene Targeting
MH  - Ion Channels/*metabolism
MH  - Mice
MH  - Mitochondria/*metabolism
MH  - Oxygen Consumption/genetics
MH  - Porins/deficiency/genetics/*metabolism
MH  - RNA/analysis
MH  - Transfection
MH  - Voltage-Dependent Anion Channel 1
MH  - Voltage-Dependent Anion Channel 2
MH  - Voltage-Dependent Anion Channels
EDAT- 1999/10/19 00:00
MHDA- 1999/10/19 00:01
CRDT- 1999/10/19 00:00
PHST- 1999/10/19 00:00 [pubmed]
PHST- 1999/10/19 00:01 [medline]
PHST- 1999/10/19 00:00 [entrez]
AID - S0167-4889(99)00120-2 [pii]
AID - 10.1016/s0167-4889(99)00120-2 [doi]
PST - ppublish
SO  - Biochim Biophys Acta. 1999 Oct 13;1452(1):68-78. doi:
      10.1016/s0167-4889(99)00120-2.