PMID- 10524337
OWN - NLM
STAT- MEDLINE
DCOM- 19991221
LR  - 20071114
IS  - 0021-9967 (Print)
IS  - 0021-9967 (Linking)
VI  - 413
IP  - 2
DP  - 1999 Oct 18
TI  - Ultrastructural localization of the corticotropin-releasing factor-binding
      protein in rat brain and pituitary.
PG  - 241-54
AB  - Preembedding immunoperoxidase staining methods were used to permit
      ultrastructural analyses of the distribution in rat brain and pituitary of the
      corticotropin-releasing factor-binding protein (CRF-BP), a moiety distinct from
      CRF receptors, but which is nonetheless capable of binding the peptide and
      reversibly neutralizing its biological actions. In anterior pituitary, CRF-BP
      immunoreactivity (ir) was detected in corticotropelike cells, with reaction
      product associated principally with secondary lysosomes and multivesicular bodies
      and not at all with secretory granules. In brain, marked regional differences in 
      the subcellular pattern of CRF-BP staining were evident. In isocortex, where
      BP/peptide colocalization is rare, BP-ir was distributed in cells and processes
      in a manner similar to that of a prototypic neuropeptide, including in terminals 
      commonly engaging in synaptic contacts with unlabeled dendritic profiles. In the 
      bed nucleus of the stria terminalis, a site that contains overlapping
      accumulations of CRF-BP-ir projections and CRF-ir perikarya, BP staining was
      restricted to vesicle-laden varicosities that rarely engaged in synaptic contacts
      with somatic or dendritic elements but were frequently apposed to unlabeled axon 
      varicosities and terminals. In the ventromedial medulla, a site of partial CRF/BP
      overlap, most cells displayed a subcellular localization CRF-BP-ir like that seen
      in cortex, whereas in others the distribution shared similarities with that
      observed in pituitary. The results suggest that the function of the CRF-BP may
      differ in different cellular contexts. In cellular targets of CRF or in neurons
      in which peptide and BP coexist, the CRF-BP may play a role in processing and
      degradation of CRF and/or ligand-receptor complexes. In other areas of the
      central nervous system, the BP seems positioned to serve as a
      transmitter/modulator at conventional synapses or as an autocrine or paracrine
      modulator of local CRF effects.
FAU - Peto, C A
AU  - Peto CA
AD  - Laboratory of Neuronal Structure and Function, Salk Institute for Biological
      Studies, La Jolla, California 92037, USA.
FAU - Arias, C
AU  - Arias C
FAU - Vale, W W
AU  - Vale WW
FAU - Sawchenko, P E
AU  - Sawchenko PE
LA  - eng
GR  - DK-26741/DK/NIDDK NIH HHS/United States
PT  - Comparative Study
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - J Comp Neurol
JT  - The Journal of comparative neurology
JID - 0406041
RN  - 0 (Carrier Proteins)
RN  - 0 (Nerve Tissue Proteins)
RN  - 134773-81-2 (corticotropin releasing factor-binding protein)
SB  - IM
MH  - Animals
MH  - *Brain Chemistry
MH  - Carrier Proteins/*analysis/physiology
MH  - Cerebral Cortex/chemistry
MH  - Cytoplasm/chemistry
MH  - Dendrites/chemistry
MH  - Lysosomes/chemistry
MH  - Medulla Oblongata/chemistry
MH  - Nerve Tissue Proteins/*analysis/physiology
MH  - Organ Specificity
MH  - Pituitary Gland, Anterior/*chemistry
MH  - Rats
MH  - Rats, Sprague-Dawley
MH  - Septal Nuclei/chemistry
MH  - Subcellular Fractions/chemistry
MH  - Synapses/chemistry
EDAT- 1999/10/19 09:00
MHDA- 2000/06/20 09:00
CRDT- 1999/10/19 09:00
PHST- 1999/10/19 09:00 [pubmed]
PHST- 2000/06/20 09:00 [medline]
PHST- 1999/10/19 09:00 [entrez]
AID - 10.1002/(SICI)1096-9861(19991018)413:2<241::AID-CNE6>3.0.CO;2-U [pii]
PST - ppublish
SO  - J Comp Neurol. 1999 Oct 18;413(2):241-54.