PMID- 10524236
OWN - NLM
STAT- MEDLINE
DCOM- 19991102
LR  - 20190707
IS  - 0378-1119 (Print)
IS  - 0378-1119 (Linking)
VI  - 237
IP  - 1
DP  - 1999 Sep 3
TI  - Five different genes, Eif4a1, Cd68, Supl15h, Sox15 and Fxr2h, are clustered in a 
      40 kb region of mouse chromosome 11.
PG  - 53-60
AB  - We characterized a region of the mouse genome disrupted by integration of a gene 
      trap (GT) vector in ES cells. On 5' rapid amplification of cDNA ends analysis of 
      the fusion transcripts containing the GT vector, we identified the eukaryotic
      protein synthesis initiation factor 4A1 gene (Eif4a1) as a promoter-trapped gene.
      Plasmid rescue was used to show that the other end of the integrated vector
      disrupted the murine homolog of the human fragile X mental retardation
      syndrome-related protein 2 gene (Fxr2h). Structural analysis of P1 clones,
      isolated from the wild-type mouse genome by PCR with Eif4a1-specific primers,
      indicated that the integration of the GT vector was accompanied by the deletion
      of about 35 kb of genomic DNA and that the disrupted region also included three
      genes, Cd68, Supl15h and Sox15, the latter two of which are transcribed in
      opposite directions with overlapping 3' ends. These five different genes at
      least, Eif4a1, Cd68, Supl15h, Sox15 and Fxr2h, are clustered in a 40 kb region.
      The chromosomal location of this region was mapped by means of interspecific
      backcross panel DNAs to the central part of mouse chromosome 11, exhibiting a
      known region of synteny with human chromosome 17.
FAU - Miyashita, A
AU  - Miyashita A
AD  - Research Laboratory for Molecular Genetics, Niigata University, Japan.
FAU - Shimizu, N
AU  - Shimizu N
FAU - Endo, N
AU  - Endo N
FAU - Hanyuu, T
AU  - Hanyuu T
FAU - Ishii, N
AU  - Ishii N
FAU - Ito, K
AU  - Ito K
FAU - Itoh, Y
AU  - Itoh Y
FAU - Shirai, M
AU  - Shirai M
FAU - Nakajima, T
AU  - Nakajima T
FAU - Odani, S
AU  - Odani S
FAU - Kuwano, R
AU  - Kuwano R
LA  - eng
SI  - GENBANK/AB009287
SI  - GENBANK/AB011595
SI  - GENBANK/AB014468
SI  - GENBANK/AB014469
SI  - GENBANK/AB014470
SI  - GENBANK/AB014471
SI  - GENBANK/AB014472
SI  - GENBANK/AB014473
SI  - GENBANK/AB014474
SI  - GENBANK/AB024713
SI  - GENBANK/AB025263
SI  - GENBANK/AB025264
SI  - GENBANK/AB025265
SI  - GENBANK/AB025266
SI  - GENBANK/AB025267
SI  - GENBANK/AB025268
SI  - GENBANK/AB025269
SI  - GENBANK/AB025311
SI  - GENBANK/AB025354
SI  - GENBANK/AB025355
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Netherlands
TA  - Gene
JT  - Gene
JID - 7706761
RN  - 0 (Antigens, CD)
RN  - 0 (Antigens, Differentiation, Myelomonocytic)
RN  - 0 (CD68 antigen, human)
RN  - 0 (DNA Transposable Elements)
RN  - 0 (FXR2 protein, human)
RN  - 0 (High Mobility Group Proteins)
RN  - 0 (Lec35 protein, Cricetulus griseus)
RN  - 0 (Peptide Initiation Factors)
RN  - 0 (RNA-Binding Proteins)
RN  - 0 (Repressor Proteins)
RN  - 0 (SOX Transcription Factors)
RN  - 0 (Sox15 protein, mouse)
RN  - 0 (Transcription Factors)
RN  - EC 2.7.7.- (Eukaryotic Initiation Factor-4A)
SB  - IM
MH  - Animals
MH  - Antigens, CD/*genetics
MH  - Antigens, Differentiation, Myelomonocytic/*genetics
MH  - Base Sequence
MH  - *Chromosome Mapping
MH  - Chromosomes/*chemistry
MH  - Chromosomes, Human, Pair 17
MH  - DNA Transposable Elements
MH  - Eukaryotic Initiation Factor-4A
MH  - Fetal Death/genetics
MH  - Genetic Vectors
MH  - High Mobility Group Proteins/*genetics
MH  - Homozygote
MH  - Humans
MH  - Mice
MH  - Mice, Inbred C57BL
MH  - Mice, Inbred Strains
MH  - Molecular Sequence Data
MH  - Multigene Family
MH  - Peptide Initiation Factors/*genetics
MH  - RNA-Binding Proteins/*genetics
MH  - Repressor Proteins/genetics
MH  - SOX Transcription Factors
MH  - Transcription Factors/*genetics
EDAT- 1999/10/19 00:00
MHDA- 1999/10/19 00:01
CRDT- 1999/10/19 00:00
PHST- 1999/10/19 00:00 [pubmed]
PHST- 1999/10/19 00:01 [medline]
PHST- 1999/10/19 00:00 [entrez]
AID - S0378-1119(99)00301-7 [pii]
AID - 10.1016/s0378-1119(99)00301-7 [doi]
PST - ppublish
SO  - Gene. 1999 Sep 3;237(1):53-60. doi: 10.1016/s0378-1119(99)00301-7.