PMID- 10524221
OWN - NLM
STAT- MEDLINE
DCOM- 19991104
LR  - 20190610
IS  - 0006-3002 (Print)
IS  - 0006-3002 (Linking)
VI  - 1446
IP  - 3
DP  - 1999 Sep 3
TI  - Cloning of ClC-2 chloride channel from murine duodenum and its presence in CFTR
      knockout mice.
PG  - 431-7
AB  - We report the cloning of a murine ClC-2 chloride channel cDNA from duodenal
      epithelium by reverse transcriptase-polymerase chain reaction (RT-PCR) using
      degenerate primers and by rapid amplification of cDNA ends (RACE)-PCR. Other than
      CFTR, this represents the first cloned chloride channel from intact intestine.
      The ClC-2 cDNA predicts encoding of a 908 amino acid polypeptide with a
      calculated M(r) of 99,373. The amino acid sequence of the murine ClC-2 chloride
      channel is over 94% identical to the ClC-2 chloride channel proteins of other
      species. Of interest is the finding that the ClC-2 mRNA is expressed about the
      same level in duodena from both CFTR knockout and wild-type mice. This is in
      keeping with the suggestion that ClC-2 might be a therapeutic target in cystic
      fibrosis.
FAU - Joo, N S
AU  - Joo NS
AD  - Department of Pharmacology, University of Missouri, Columbia 65212, USA.
FAU - Clarke, L L
AU  - Clarke LL
FAU - Han, B H
AU  - Han BH
FAU - Forte, L R
AU  - Forte LR
FAU - Kim, H D
AU  - Kim HD
LA  - eng
GR  - R29 DK048816/DK/NIDDK NIH HHS/United States
GR  - DK-48816/DK/NIDDK NIH HHS/United States
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - Netherlands
TA  - Biochim Biophys Acta
JT  - Biochimica et biophysica acta
JID - 0217513
RN  - 0 (Chloride Channels)
RN  - 0 (ClC-2 chloride channels)
RN  - 0 (DNA, Complementary)
RN  - 126880-72-6 (Cystic Fibrosis Transmembrane Conductance Regulator)
RN  - 63231-63-0 (RNA)
SB  - IM
MH  - Amino Acid Sequence
MH  - Animals
MH  - Base Sequence
MH  - Blotting, Northern
MH  - Chloride Channels/*genetics
MH  - Cloning, Molecular
MH  - Cystic Fibrosis Transmembrane Conductance Regulator/genetics
MH  - DNA, Complementary/chemistry/isolation & purification
MH  - Duodenum/*metabolism
MH  - Intestinal Mucosa/metabolism
MH  - Mice
MH  - Mice, Knockout
MH  - Molecular Sequence Data
MH  - Plasmids
MH  - RNA/chemistry/isolation & purification
MH  - Reverse Transcriptase Polymerase Chain Reaction
MH  - Sequence Alignment
EDAT- 1999/10/19 00:00
MHDA- 1999/10/19 00:01
CRDT- 1999/10/19 00:00
PHST- 1999/10/19 00:00 [pubmed]
PHST- 1999/10/19 00:01 [medline]
PHST- 1999/10/19 00:00 [entrez]
AID - S0167-4781(99)00110-4 [pii]
AID - 10.1016/s0167-4781(99)00110-4 [doi]
PST - ppublish
SO  - Biochim Biophys Acta. 1999 Sep 3;1446(3):431-7. doi:
      10.1016/s0167-4781(99)00110-4.