PMID- 10524211
OWN - NLM
STAT- MEDLINE
DCOM- 19991104
LR  - 20190610
IS  - 0006-3002 (Print)
IS  - 0006-3002 (Linking)
VI  - 1446
IP  - 3
DP  - 1999 Sep 3
TI  - Isolation, molecular characterization, and tissue-specific expression of ECP-51
      and ECP-54 (TIP49), two homologous, interacting erythroid cytosolic proteins.
PG  - 365-70
AB  - We isolated two proteins, ECP-51 and ECP-54, from human erythrocyte cytosol by
      affinity chromatography using a peptide of the integral membrane protein stomatin
      as bait. Partial amino acid sequence information obtained by microsequencing
      allowed us to clone the respective cDNAs. Analysis of the nucleotide sequences
      revealed that ECP-51 and ECP-54 are homologous (44.2% amino acid identity) and
      contain ATP-binding sites. ECP-54 was identified as TIP49/RUVBL1/NMP238, which is
      a component of a large nuclear protein complex, possibly the RNA polymerase II
      holoenzyme; ECP-51 is a novel protein. Using the two-hybrid system, we showed
      that these proteins interact with each other. The interaction of ECP-51 and
      ECP-54 with the stomatin peptide and the localization to the nucleus and
      cytoplasm suggest an additional function for these proteins as chaperone
      components.
FAU - Salzer, U
AU  - Salzer U
AD  - Institute of Biochemistry, Medical Faculty, University of Vienna, Austria.
FAU - Kubicek, M
AU  - Kubicek M
FAU - Prohaska, R
AU  - Prohaska R
LA  - eng
SI  - GENBANK/Y18417
SI  - GENBANK/Y18418
PT  - Comparative Study
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Netherlands
TA  - Biochim Biophys Acta
JT  - Biochimica et biophysica acta
JID - 0217513
RN  - 0 (Blood Proteins)
RN  - 0 (Carrier Proteins)
RN  - 0 (DNA Primers)
RN  - 0 (Membrane Proteins)
RN  - 0 (STOM protein, human)
RN  - EC 3.6.4.- (ATPases Associated with Diverse Cellular Activities)
RN  - EC 3.6.4.- (DNA Helicases)
RN  - EC 3.6.4.12 (RUVBL1 protein, human)
RN  - EC 3.6.4.12 (RUVBL2 protein, human)
SB  - IM
MH  - ATPases Associated with Diverse Cellular Activities
MH  - Amino Acid Sequence
MH  - Blood Proteins/metabolism
MH  - Carrier Proteins/chemistry/*genetics/isolation & purification
MH  - Chromatography, Affinity
MH  - Cloning, Molecular
MH  - Cytosol/metabolism
MH  - *DNA Helicases
MH  - DNA Primers
MH  - Databases as Topic
MH  - Erythrocytes/*metabolism
MH  - Humans
MH  - *Membrane Proteins
MH  - Molecular Sequence Data
MH  - Polymerase Chain Reaction
MH  - Sequence Alignment
MH  - Sequence Homology, Amino Acid
EDAT- 1999/10/19 00:00
MHDA- 1999/10/19 00:01
CRDT- 1999/10/19 00:00
PHST- 1999/10/19 00:00 [pubmed]
PHST- 1999/10/19 00:01 [medline]
PHST- 1999/10/19 00:00 [entrez]
AID - S0167-4781(99)00104-9 [pii]
AID - 10.1016/s0167-4781(99)00104-9 [doi]
PST - ppublish
SO  - Biochim Biophys Acta. 1999 Sep 3;1446(3):365-70. doi:
      10.1016/s0167-4781(99)00104-9.