PMID- 10524201
OWN - NLM
STAT- MEDLINE
DCOM- 19991104
LR  - 20190610
IS  - 0006-3002 (Print)
IS  - 0006-3002 (Linking)
VI  - 1446
IP  - 3
DP  - 1999 Sep 3
TI  - Isolation and characterization of the distal promoter region of mouse Cbfa1.
PG  - 265-72
AB  - Cbfa1 is an essential transcription factor for bone formation, and as such little
      is known about the region responsible for the transcriptional regulation of this 
      gene. Here we report the determination of the transcription start sites,
      isolation and partial characterization of distal promoter region of this gene.
      Three transcription start sites were identified by the 5'-Cap site method,
      recently invented for rapid examination of the 5'-end of genes of interest. A
      reporter construct containing 1.8 kb of 5' of transcription start sites had
      approximately 25-fold more luciferase activity than the promoter-less vector in
      osteoblastic cell lines. Deletion analysis of the reporter construct demonstrated
      that the minimal region to express promoter activity lies between bp -168 and
      -99, taking the most downstream transcription start site as +1. By Northern blot 
      analysis, mRNA expression from the distal promoter was detected in the
      differentiated osteoblastic cell lines, UMR-106, ROS17/2.8 and MC3T3-E1, but not 
      in cell lines of immature phenotype or originated from other organs. Luciferase
      activity was strongest in UMR-106 and ROS17/2.8, and weakest in COS-1 and HepG2, 
      which are cell lines originating from other organs, corresponding to the level of
      mRNA expression. These results demonstrated that the distal promoter region
      examined here is important for tissue- and cell-type-specific gene expression of 
      Cbfa1.
FAU - Fujiwara, M
AU  - Fujiwara M
AD  - Sumitomo Pharmaceuticals Research Center, Osaka, Japan.
FAU - Tagashira, S
AU  - Tagashira S
FAU - Harada, H
AU  - Harada H
FAU - Ogawa, S
AU  - Ogawa S
FAU - Katsumata, T
AU  - Katsumata T
FAU - Nakatsuka, M
AU  - Nakatsuka M
FAU - Komori, T
AU  - Komori T
FAU - Takada, H
AU  - Takada H
LA  - eng
SI  - GENBANK/AB013129
PT  - Comparative Study
PT  - Journal Article
PL  - Netherlands
TA  - Biochim Biophys Acta
JT  - Biochimica et biophysica acta
JID - 0217513
RN  - 0 (Core Binding Factor Alpha 1 Subunit)
RN  - 0 (DNA-Binding Proteins)
RN  - 0 (Neoplasm Proteins)
RN  - 0 (RNA, Messenger)
RN  - 0 (Transcription Factors)
SB  - IM
MH  - Animals
MH  - Base Sequence
MH  - Cell Line
MH  - Cloning, Molecular
MH  - Core Binding Factor Alpha 1 Subunit
MH  - DNA-Binding Proteins/*genetics
MH  - Haplorhini
MH  - Humans
MH  - Mice
MH  - Molecular Sequence Data
MH  - *Neoplasm Proteins
MH  - Plasmids
MH  - Polymerase Chain Reaction
MH  - Promoter Regions, Genetic
MH  - RNA, Messenger/genetics/isolation & purification
MH  - Rats
MH  - Transcription Factors/*genetics
MH  - Transfection
EDAT- 1999/10/19 00:00
MHDA- 1999/10/19 00:01
CRDT- 1999/10/19 00:00
PHST- 1999/10/19 00:00 [pubmed]
PHST- 1999/10/19 00:01 [medline]
PHST- 1999/10/19 00:00 [entrez]
AID - S0167-4781(99)00113-X [pii]
AID - 10.1016/s0167-4781(99)00113-x [doi]
PST - ppublish
SO  - Biochim Biophys Acta. 1999 Sep 3;1446(3):265-72. doi:
      10.1016/s0167-4781(99)00113-x.