PMID- 10524196
OWN - NLM
STAT- MEDLINE
DCOM- 19991104
LR  - 20190610
IS  - 0006-3002 (Print)
IS  - 0006-3002 (Linking)
VI  - 1446
IP  - 3
DP  - 1999 Sep 3
TI  - Genomic structure and promoter analysis of the ecto-phosphodiesterase I gene
      (PDNP3) expressed in glial cells.
PG  - 213-24
AB  - PDNP (phosphodiesterase I/nucleotide pyrophosphatase) is one of a series of
      ectoenzymes that are involved in hydrolysis of extracellular nucleotides. PDNP
      possesses ATPase (EC 3.6.1.3) and ATP pyrophosphatase (EC 3.6.1.8) activities.
      Mammalian PDNP consists of three closely related family proteins (PDNP1, -2, and 
      -3), and they are expressed in different cell types and at different
      developmental stages. Rat PDNP3 is expressed in a subset of immature glial cells 
      and in the alimentary tract. Human PDNP3 is expressed in glioma cells, prostate, 
      and uterus, but not in the alimentary tract. We have cloned genomic DNA
      containing the whole coding region of the human PDNP3 gene and determined its
      exon-intron structure. The human PDNP3 gene spans over 60 kb and is organized
      into 25 exons and 24 introns. We determined the nucleotide sequence of the
      5'-flanking region of human and rat PDNP3 genes. The upstream region of both
      species lacks a canonical TATA box and contains a putative binding site for CCAAT
      enhancer-binding proteins near the transcription start site. Promoter activity
      analysis of the 5'-flanking region revealed that the sequence around the CCAAT
      box is required for its transcriptional activity in 9L rat glioma cells. A gel
      shift assay demonstrated that 9L nuclear extract contains proteins that bind to
      this region.
FAU - Andoh, K
AU  - Andoh K
AD  - Department of Pediatrics, Kobe University School of Medicine, Japan.
FAU - Piao, J H
AU  - Piao JH
FAU - Terashima, K
AU  - Terashima K
FAU - Nakamura, H
AU  - Nakamura H
FAU - Sano, K
AU  - Sano K
LA  - eng
SI  - GENBANK/AF119714
PT  - Comparative Study
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Netherlands
TA  - Biochim Biophys Acta
JT  - Biochimica et biophysica acta
JID - 0217513
RN  - 0 (DNA, Complementary)
RN  - EC 3.1.4.- (Phosphoric Diester Hydrolases)
RN  - EC 3.1.4.1 (Phosphodiesterase I)
RN  - EC 3.6.1.- (Pyrophosphatases)
RN  - EC 3.6.1.9 (nucleotide pyrophosphatase)
SB  - IM
MH  - Animals
MH  - Base Sequence
MH  - Cell Line
MH  - Cloning, Molecular
MH  - Colon/metabolism
MH  - DNA, Complementary/chemistry/isolation & purification
MH  - Exons
MH  - Humans
MH  - Intestine, Small/metabolism
MH  - Introns
MH  - Liver/metabolism
MH  - Molecular Sequence Data
MH  - Neuroglia/enzymology/*metabolism
MH  - Pancreas/metabolism
MH  - Phosphodiesterase I
MH  - Phosphoric Diester Hydrolases/*genetics
MH  - Promoter Regions, Genetic
MH  - Pyrophosphatases/*genetics
MH  - Rats
MH  - Reverse Transcriptase Polymerase Chain Reaction
EDAT- 1999/10/19 00:00
MHDA- 1999/10/19 00:01
CRDT- 1999/10/19 00:00
PHST- 1999/10/19 00:00 [pubmed]
PHST- 1999/10/19 00:01 [medline]
PHST- 1999/10/19 00:00 [entrez]
AID - S0167-4781(99)00090-1 [pii]
AID - 10.1016/s0167-4781(99)00090-1 [doi]
PST - ppublish
SO  - Biochim Biophys Acta. 1999 Sep 3;1446(3):213-24. doi:
      10.1016/s0167-4781(99)00090-1.