PMID- 10523663
OWN - NLM
STAT- MEDLINE
DCOM- 19991124
LR  - 20190508
IS  - 0270-7306 (Print)
IS  - 0270-7306 (Linking)
VI  - 19
IP  - 11
DP  - 1999 Nov
TI  - Two new members of the emerging KDWK family of combinatorial transcription
      modulators bind as a heterodimer to flexibly spaced PuCGPy half-sites.
PG  - 7741-50
AB  - Initially recognized as a HeLa factor essential for parvovirus DNA replication,
      parvovirus initiation factor (PIF) is a site-specific DNA-binding complex
      consisting of p96 and p79 subunits. We have cloned and sequenced the human cDNAs 
      encoding each subunit and characterized their products expressed from recombinant
      baculoviruses. The p96 and p79 polypeptides have 40% amino acid identity, focused
      particularly within a 94-residue region containing the sequence KDWK. This motif,
      first described for the Drosophila homeobox activator DEAF-1, identifies an
      emerging group of metazoan transcriptional modulators. During viral replication, 
      PIF critically regulates the viral nickase, but in the host cell it probably
      modulates transcription, since each subunit is active in promoter activation
      assays and the complex binds to previously described regulatory elements in the
      tyrosine aminotransferase and transferrin receptor promoters. Within its
      recognition site, PIF binds coordinately to two copies of the tetranucleotide
      PuCGPy, which, remarkably, can be spaced from 1 to 15 nucleotides apart, a novel 
      flexibility that we suggest may be characteristic of the KDWK family. Such
      tetranucleotides are common in promoter regions, particularly in activating
      transcription factor/cyclic AMP response element-binding protein (ATF/CREB) and
      E-box motifs, suggesting that PIF may modulate the transcription of many genes.
FAU - Christensen, J
AU  - Christensen J
AD  - Department of Laboratory Medicine, Yale University School of Medicine, New Haven,
      Connecticut 06510, USA.
FAU - Cotmore, S F
AU  - Cotmore SF
FAU - Tattersall, P
AU  - Tattersall P
LA  - eng
SI  - GENBANK/AF173867
SI  - GENBANK/AF173868
GR  - AI26109/AI/NIAID NIH HHS/United States
GR  - CA29303/CA/NCI NIH HHS/United States
GR  - R37 AI026109/AI/NIAID NIH HHS/United States
GR  - R01 AI026109/AI/NIAID NIH HHS/United States
GR  - R01 CA029303/CA/NCI NIH HHS/United States
PT  - Comparative Study
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - Mol Cell Biol
JT  - Molecular and cellular biology
JID - 8109087
RN  - 0 (DNA, Complementary)
RN  - 0 (DNA-Binding Proteins)
RN  - 0 (Nuclear Proteins)
RN  - 0 (Receptors, Transferrin)
RN  - 0 (Recombinant Proteins)
RN  - 0 (Transcription Factors)
RN  - 0 (p79 subunit, parvovirus initiation factor)
RN  - 0 (p96 subunit, parvovirus initiation factor)
RN  - EC 2.6.1.5 (Tyrosine Transaminase)
SB  - IM
MH  - *Amino Acid Motifs
MH  - Amino Acid Sequence
MH  - Binding Sites
MH  - Cloning, Molecular
MH  - DNA, Complementary/genetics
MH  - DNA-Binding Proteins/genetics/isolation & purification/*metabolism
MH  - Dimerization
MH  - *GC Rich Sequence
MH  - HeLa Cells
MH  - Humans
MH  - Molecular Sequence Data
MH  - *Multigene Family
MH  - Nuclear Proteins/genetics/isolation & purification/metabolism
MH  - Parvovirus/genetics
MH  - Promoter Regions, Genetic
MH  - Protein Binding
MH  - Receptors, Transferrin/genetics
MH  - Recombinant Proteins/metabolism
MH  - Replication Origin
MH  - Sequence Homology, Amino Acid
MH  - Transcription Factors/genetics/isolation & purification/*metabolism
MH  - Tyrosine Transaminase/genetics
PMC - PMC84824
EDAT- 1999/10/19 00:00
MHDA- 1999/10/19 00:01
CRDT- 1999/10/19 00:00
PHST- 1999/10/19 00:00 [pubmed]
PHST- 1999/10/19 00:01 [medline]
PHST- 1999/10/19 00:00 [entrez]
AID - 10.1128/mcb.19.11.7741 [doi]
PST - ppublish
SO  - Mol Cell Biol. 1999 Nov;19(11):7741-50. doi: 10.1128/mcb.19.11.7741.