PMID- 10523658
OWN - NLM
STAT- MEDLINE
DCOM- 19991124
LR  - 20190508
IS  - 0270-7306 (Print)
IS  - 0270-7306 (Linking)
VI  - 19
IP  - 11
DP  - 1999 Nov
TI  - The TFIIIC90 subunit of TFIIIC interacts with multiple components of the RNA
      polymerase III machinery and contains a histone-specific acetyltransferase
      activity.
PG  - 7697-704
AB  - Human transcription factor IIIC (hTFIIIC) is a multisubunit complex that directly
      recognizes promoter elements and recruits TFIIIB and RNA polymerase III. Here we 
      describe the cDNA cloning and characterization of the 90-kDa subunit (hTFIIIC90) 
      that is present within a DNA-binding subcomplex (TFIIIC2) of TFIIIC. hTFIIIC90
      has no specific homology to any of the known yeast TFIIIC subunits.
      Immunodepletion and immunoprecipitation studies indicate that hTFIIIC90 is a bona
      fide subunit of TFIIIC2 and absolutely required for RNA polymerase III
      transcription. hTFIIIC90 shows interactions with the hTFIIIC220, hTFIIIC110, and 
      hTFIIIC63 subunits of TFIIIC, the hTFIIIB90 subunit of TFIIIB, and the human
      RPC39 (hRPC39) and hRPC62 subunits of an initiation-specific subcomplex of RNA
      polymerase III. These interactions may facilitate both TFIIIB and RNA polymerase 
      III recruitment to the preinitiation complex by TFIIIC. We show that hTFIIIC90
      has an intrinsic histone acetyltransferase activity with a substrate specificity 
      for histone H3.
FAU - Hsieh, Y J
AU  - Hsieh YJ
AD  - Laboratory of Biochemistry, The Rockefeller University, New York, New York 10021,
      USA.
FAU - Kundu, T K
AU  - Kundu TK
FAU - Wang, Z
AU  - Wang Z
FAU - Kovelman, R
AU  - Kovelman R
FAU - Roeder, R G
AU  - Roeder RG
LA  - eng
SI  - GENBANK/AF142328
GR  - CA42567/CA/NCI NIH HHS/United States
PT  - Journal Article
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - Mol Cell Biol
JT  - Molecular and cellular biology
JID - 8109087
RN  - 0 (DNA, Complementary)
RN  - 0 (Histones)
RN  - 0 (Saccharomyces cerevisiae Proteins)
RN  - 0 (Transcription Factor TFIIIB)
RN  - 0 (Transcription Factors)
RN  - 0 (Transcription Factors, TFIII)
RN  - 0 (transcription factor TFIIIC)
RN  - EC 2.3.1.- (Acetyltransferases)
RN  - EC 2.3.1.48 (Histone Acetyltransferases)
RN  - EC 2.7.7.6 (RNA Polymerase III)
SB  - IM
MH  - Acetylation
MH  - Acetyltransferases/genetics/*metabolism
MH  - Amino Acid Sequence
MH  - Cloning, Molecular
MH  - DNA, Complementary/genetics
MH  - Histone Acetyltransferases
MH  - Histones/*metabolism
MH  - Humans
MH  - Molecular Sequence Data
MH  - Precipitin Tests
MH  - Protein Binding
MH  - RNA Polymerase III/*metabolism
MH  - *Saccharomyces cerevisiae Proteins
MH  - Sequence Analysis, DNA
MH  - Substrate Specificity
MH  - Transcription Factor TFIIIB
MH  - Transcription Factors/genetics/*metabolism
MH  - *Transcription Factors, TFIII
MH  - Transcription, Genetic
PMC - PMC84812
EDAT- 1999/10/19 00:00
MHDA- 1999/10/19 00:01
CRDT- 1999/10/19 00:00
PHST- 1999/10/19 00:00 [pubmed]
PHST- 1999/10/19 00:01 [medline]
PHST- 1999/10/19 00:00 [entrez]
AID - 10.1128/mcb.19.11.7697 [doi]
PST - ppublish
SO  - Mol Cell Biol. 1999 Nov;19(11):7697-704. doi: 10.1128/mcb.19.11.7697.