PMID- 10523320
OWN - NLM
STAT- MEDLINE
DCOM- 19991206
LR  - 20141120
IS  - 0261-4189 (Print)
IS  - 0261-4189 (Linking)
VI  - 18
IP  - 20
DP  - 1999 Oct 15
TI  - A doughnut-shaped heteromer of human Sm-like proteins binds to the 3'-end of U6
      snRNA, thereby facilitating U4/U6 duplex formation in vitro.
PG  - 5789-802
AB  - We describe the isolation and molecular characterization of seven distinct
      proteins present in human [U4/U6.U5] tri-snRNPs. These proteins exhibit clear
      homology to the Sm proteins and are thus denoted LSm (like Sm) proteins. Purified
      LSm proteins form a heteromer that is stable even in the absence of RNA and
      exhibits a doughnut shape under the electron microscope, with striking similarity
      to the Sm core RNP structure. The purified LSm heteromer binds specifically to U6
      snRNA, requiring the 3'-terminal U-tract for complex formation. The 3'-end of U6 
      snRNA was also co-precipitated with LSm proteins after digestion of isolated
      tri-snRNPs with RNaseT(1). Importantly, the LSm proteins did not bind to the
      U-rich Sm sites of intact U1, U2, U4 or U5 snRNAs, indicating that they can only 
      interact with a 3'-terminal U-tract. Finally, we show that the LSm proteins
      facilitate the formation of U4/U6 RNA duplices in vitro, suggesting that the LSm 
      proteins may play a role in U4/U6 snRNP formation.
FAU - Achsel, T
AU  - Achsel T
AD  - Institut fur Molekularbiologie und Tumorforschung, Universitat Marburg, Emil
      Mannkopff-Strasse 2, 35037 Marburg, Germany.
FAU - Brahms, H
AU  - Brahms H
FAU - Kastner, B
AU  - Kastner B
FAU - Bachi, A
AU  - Bachi A
FAU - Wilm, M
AU  - Wilm M
FAU - Luhrmann, R
AU  - Luhrmann R
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - EMBO J
JT  - The EMBO journal
JID - 8208664
RN  - 0 (RNA, Small Nuclear)
RN  - 0 (Ribonucleoprotein, U4-U6 Small Nuclear)
SB  - IM
MH  - Amino Acid Sequence
MH  - Base Sequence
MH  - Binding Sites/genetics
MH  - HeLa Cells
MH  - Humans
MH  - In Vitro Techniques
MH  - Microscopy, Electron
MH  - Molecular Sequence Data
MH  - Nucleic Acid Conformation
MH  - Protein Binding
MH  - Protein Structure, Quaternary
MH  - RNA Splicing
MH  - RNA, Small Nuclear/*chemistry/genetics/*metabolism
MH  - Ribonucleoprotein, U4-U6 Small Nuclear/*chemistry/genetics/*metabolism
MH  - Sequence Homology, Amino Acid
MH  - Spliceosomes/metabolism
PMC - PMC1171645
EDAT- 1999/10/16 00:00
MHDA- 1999/10/16 00:01
CRDT- 1999/10/16 00:00
PHST- 1999/10/16 00:00 [pubmed]
PHST- 1999/10/16 00:01 [medline]
PHST- 1999/10/16 00:00 [entrez]
AID - 10.1093/emboj/18.20.5789 [doi]
PST - ppublish
SO  - EMBO J. 1999 Oct 15;18(20):5789-802. doi: 10.1093/emboj/18.20.5789.