PMID- 10523317
OWN - NLM
STAT- MEDLINE
DCOM- 19991206
LR  - 20161124
IS  - 0261-4189 (Print)
IS  - 0261-4189 (Linking)
VI  - 18
IP  - 20
DP  - 1999 Oct 15
TI  - The replication factory targeting sequence/PCNA-binding site is required in G(1) 
      to control the phosphorylation status of DNA ligase I.
PG  - 5745-54
AB  - The recruitment of DNA ligase I to replication foci in S phase depends on a
      replication factory targeting sequence that also mediates the interaction with
      proliferating cell nuclear antigen (PCNA) in vitro. By exploiting a monoclonal
      antibody directed at a phospho-epitope, we demonstrate that Ser66 of DNA ligase
      I, which is part of a strong CKII consensus site, is phosphorylated in a cell
      cycle-dependent manner. After dephosphorylation in early G(1), the level of Ser66
      phosphorylation is minimal in G(1), increases progressively in S and peaks in
      G(2)/M phase. The analysis of epitope-tagged DNA ligase I mutants demonstrates
      that dephosphorylation of Ser66 requires both the nuclear localization and the
      PCNA-binding site of the enzyme. Finally, we show that DNA ligase I and PCNA
      interact in vivo in G(1) and S phase but not in G(2)/M. We propose that
      dephosphorylation of Ser66 is part of a novel control mechanism to establish the 
      pre-replicative form of DNA ligase I.
FAU - Rossi, R
AU  - Rossi R
AD  - Istituto di Genetica Biochimica ed Evoluzionistica, CNR, Via Abbiategrasso 207,
      27100 Pavia, Italy.
FAU - Villa, A
AU  - Villa A
FAU - Negri, C
AU  - Negri C
FAU - Scovassi, I
AU  - Scovassi I
FAU - Ciarrocchi, G
AU  - Ciarrocchi G
FAU - Biamonti, G
AU  - Biamonti G
FAU - Montecucco, A
AU  - Montecucco A
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - EMBO J
JT  - The EMBO journal
JID - 8208664
RN  - 0 (Antibodies, Monoclonal)
RN  - 0 (Epitopes)
RN  - 0 (LIG1 protein, human)
RN  - 0 (Proliferating Cell Nuclear Antigen)
RN  - 452VLY9402 (Serine)
RN  - EC 6.5.1.- (DNA Ligases)
RN  - EC 6.5.1.1 (DNA Ligase ATP)
SB  - IM
MH  - 3T3 Cells
MH  - Animals
MH  - Antibodies, Monoclonal
MH  - Binding Sites
MH  - COS Cells
MH  - Cell Line
MH  - DNA Ligase ATP
MH  - DNA Ligases/chemistry/immunology/*metabolism
MH  - DNA Replication/*physiology
MH  - Epitopes/chemistry
MH  - G1 Phase/*physiology
MH  - HeLa Cells
MH  - Humans
MH  - Mice
MH  - Phosphorylation
MH  - Proliferating Cell Nuclear Antigen/*metabolism
MH  - S Phase/physiology
MH  - Serine/chemistry
PMC - PMC1171641
EDAT- 1999/10/16 00:00
MHDA- 1999/10/16 00:01
CRDT- 1999/10/16 00:00
PHST- 1999/10/16 00:00 [pubmed]
PHST- 1999/10/16 00:01 [medline]
PHST- 1999/10/16 00:00 [entrez]
AID - 10.1093/emboj/18.20.5745 [doi]
PST - ppublish
SO  - EMBO J. 1999 Oct 15;18(20):5745-54. doi: 10.1093/emboj/18.20.5745.