PMID- 10523300
OWN - NLM
STAT- MEDLINE
DCOM- 19991206
LR  - 20191210
IS  - 0261-4189 (Print)
IS  - 0261-4189 (Linking)
VI  - 18
IP  - 20
DP  - 1999 Oct 15
TI  - HLS7, a hemopoietic lineage switch gene homologous to the leukemia-inducing gene 
      MLF1.
PG  - 5559-66
AB  - Hemopoietic lineage switching occurs when leukemic cells, apparently committed to
      one lineage, change and display the phenotype of another pathway. cDNA
      representational difference analysis was used to identify myeloid-specific genes 
      that may be associated with an erythroid to myeloid lineage switch involving the 
      murine J2E erythroleukemic cell line. One of the genes isolated (HLS7) is
      homologous to the novel human oncogene myeloid leukemia factor 1 (MLF1) involved 
      in the t(3;5)(q25.1;q34) translocation associated with acute myeloid leukemia.
      Enforced expression of HLS7 in J2E cells induced a monoblastoid phenotype,
      thereby recapitulating the spontaneous erythroid to myeloid lineage switch. HLS7 
      also inhibited erythropoietin- or chemically-induced differentiation of
      erythroleukemic cell lines and suppressed development of
      erythropoietin-responsive colonies in semi-solid culture. However, intracellular 
      signaling activated by erythropoietin was not impeded by ectopic expression of
      HLS7. In contrast, HLS7 promoted maturation of M1 monoblastoid cells and
      increased myeloid colony formation in vitro. These data show that HLS7 can
      influence erythroid/myeloid lineage switching and the development of normal
      hemopoietic cells.
FAU - Williams, J H
AU  - Williams JH
AD  - Laboratory for Cancer Medicine, Department of Biochemistry, Royal Perth Hospital,
      Perth, Western Australia.
FAU - Daly, L N
AU  - Daly LN
FAU - Ingley, E
AU  - Ingley E
FAU - Beaumont, J G
AU  - Beaumont JG
FAU - Tilbrook, P A
AU  - Tilbrook PA
FAU - Lalonde, J P
AU  - Lalonde JP
FAU - Stillitano, J P
AU  - Stillitano JP
FAU - Klinken, S P
AU  - Klinken SP
LA  - eng
SI  - GENBANK/AF009515
PT  - Comparative Study
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - EMBO J
JT  - The EMBO journal
JID - 8208664
RN  - 0 (DNA Primers)
RN  - 0 (DNA-Binding Proteins)
RN  - 0 (MLF1 protein, human)
RN  - 0 (Mlf1 protein, mouse)
RN  - 0 (Proteins)
SB  - IM
MH  - Amino Acid Sequence
MH  - Animals
MH  - Base Sequence
MH  - DNA Primers/genetics
MH  - DNA-Binding Proteins
MH  - Gene Expression
MH  - *Genes, Switch
MH  - Hematopoiesis/*genetics
MH  - Humans
MH  - Leukemia/*genetics
MH  - Leukemia, Erythroblastic, Acute/genetics/pathology
MH  - Leukemia, Myeloid, Acute/genetics/pathology
MH  - Mice
MH  - Molecular Sequence Data
MH  - *Oncogenes
MH  - Phenotype
MH  - Proteins/*genetics
MH  - Sequence Homology, Amino Acid
MH  - Translocation, Genetic
MH  - Tumor Cells, Cultured
PMC - PMC1171624
EDAT- 1999/10/16 00:00
MHDA- 1999/10/16 00:01
CRDT- 1999/10/16 00:00
PHST- 1999/10/16 00:00 [pubmed]
PHST- 1999/10/16 00:01 [medline]
PHST- 1999/10/16 00:00 [entrez]
AID - 10.1093/emboj/18.20.5559 [doi]
PST - ppublish
SO  - EMBO J. 1999 Oct 15;18(20):5559-66. doi: 10.1093/emboj/18.20.5559.