PMID- 10521600
OWN - NLM
STAT- MEDLINE
DCOM- 19991222
LR  - 20190826
IS  - 0169-328X (Print)
IS  - 0169-328X (Linking)
VI  - 72
IP  - 1
DP  - 1999 Sep 8
TI  - Identification of NFI-binding sites and cloning of NFI-cDNAs suggest a regulatory
      role for NFI transcription factors in olfactory neuron gene expression.
PG  - 65-79
AB  - Olfactory receptor neurons are responsible for the detection and signal
      transduction of odor ligands. Several genes associated with this activity are
      preferentially or exclusively expressed in these neurons. Among these genes are
      those coding for olfactory receptors, adenylyl cyclase type III, the cyclic
      nucleotide gated olfactory channel 1 (OcNC-1), Galpha(olf) and the olfactory
      marker protein (OMP). Promoter analyses of these genes identified a binding site 
      for the new transcription factor family O/E whose initial member, Olf-1, is
      abundantly expressed in olfactory neurons. We report here that the proximal
      promoters of three of these genes, that are selectively expressed in olfactory
      neurons, each contains a functional NFI binding site and that the sites have
      different affinities for NFI proteins indicating a regulatory role for NFI
      proteins in olfactory gene expression. We further demonstrate, by cloning, that
      all four NFI genes are expressed in the olfactory nasal mucosa. Analysis by in
      situ hybridization illustrates that at least three of these gene products are
      expressed in the neuroepithelium in which the olfactory neurons reside. NFI
      proteins are capable of functioning as positive or negative regulators of
      transcription depending on the tissue, cell-type, age, and gene in question.
      These multivalent functions of NFI could be achieved by temporally and spatially 
      regulated expression of distinct subsets of NFI isoforms. It now remains to
      characterize the tissue and cell specific patterns of expression of distinct NFI 
      transcription factors during ontogeny and their roles in regulating gene
      expression.
FAU - Baumeister, H
AU  - Baumeister H
AD  - Deutsches Institut fur Ernahrungsforschung, Bergholz-Rehbrucke, 14558, Germany.
FAU - Gronostajski, R M
AU  - Gronostajski RM
FAU - Lyons, G E
AU  - Lyons GE
FAU - Margolis, F L
AU  - Margolis FL
LA  - eng
SI  - GENBANK/AF112455
SI  - GENBANK/AF112456
SI  - GENBANK/AF112457
SI  - GENBANK/AF112458
SI  - GENBANK/AF112459
GR  - DC03112/DC/NIDCD NIH HHS/United States
GR  - HD34908/HD/NICHD NIH HHS/United States
PT  - Comparative Study
PT  - Journal Article
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - Netherlands
TA  - Brain Res Mol Brain Res
JT  - Brain research. Molecular brain research
JID - 8908640
RN  - 0 (DNA, Complementary)
RN  - 0 (DNA-Binding Proteins)
RN  - 0 (Ebf1 protein, mouse)
RN  - 0 (Ebf1 protein, rat)
RN  - 0 (NFI Transcription Factors)
RN  - 0 (NFI-A2 protein, rat)
RN  - 0 (Nerve Tissue Proteins)
RN  - 0 (Nfia protein, rat)
RN  - 0 (Nfic protein, rat)
RN  - 0 (Protein Isoforms)
RN  - 0 (Repressor Proteins)
RN  - 0 (Trans-Activators)
RN  - 0 (Transcription Factors)
SB  - IM
MH  - Amino Acid Sequence
MH  - Animals
MH  - Base Sequence
MH  - Binding Sites
MH  - Cloning, Molecular
MH  - DNA, Complementary/genetics
MH  - DNA-Binding Proteins/*physiology
MH  - Epithelial Cells/metabolism
MH  - *Gene Expression Regulation
MH  - *Genes
MH  - In Situ Hybridization
MH  - Mice
MH  - Molecular Sequence Data
MH  - Multigene Family
MH  - NFI Transcription Factors
MH  - Nerve Tissue Proteins/genetics/*physiology
MH  - Olfactory Mucosa/metabolism
MH  - Olfactory Receptor Neurons/*metabolism
MH  - Promoter Regions, Genetic
MH  - Protein Isoforms/genetics/*physiology
MH  - Rats
MH  - Repressor Proteins/genetics/*physiology
MH  - Reverse Transcriptase Polymerase Chain Reaction
MH  - Sequence Alignment
MH  - Sequence Homology, Nucleic Acid
MH  - Species Specificity
MH  - Trans-Activators/*physiology
MH  - Transcription Factors/*chemistry/genetics
EDAT- 1999/10/16 00:00
MHDA- 1999/10/16 00:01
CRDT- 1999/10/16 00:00
PHST- 1999/10/16 00:00 [pubmed]
PHST- 1999/10/16 00:01 [medline]
PHST- 1999/10/16 00:00 [entrez]
AID - S0169328X99002107 [pii]
AID - 10.1016/s0169-328x(99)00210-7 [doi]
PST - ppublish
SO  - Brain Res Mol Brain Res. 1999 Sep 8;72(1):65-79. doi:
      10.1016/s0169-328x(99)00210-7.