PMID- 10521539
OWN - NLM
STAT- MEDLINE
DCOM- 19991122
LR  - 20190513
IS  - 0959-6658 (Print)
IS  - 0959-6658 (Linking)
VI  - 9
IP  - 10
DP  - 1999 Oct
TI  - Mice infected with Schistosoma mansoni generate antibodies to LacdiNAc (GalNAc
      beta 1-->4GlcNAc) determinants.
PG  - 1029-35
AB  - Schistosoma mansoni is a parasitic trematode infecting humans and animals. We
      reported previously that adult S. mansoni synthesizes complex type biantennary
      N-glycans bearing the terminal sequence GalNAc beta 1-->4GlcNAc-R (lacdiNAc or
      LDN). We now report that mice infected with S. mansoni generate antibodies to
      LDN, as assessed by ELISA using a synthetic neoglycoconjugate containing LDN
      sequences. Sera of infected mice, but not uninfected mice, contained primarily
      IgM and low levels of IgG toward LDN. Interestingly, these antibodies also
      recognize bovine milk glycoproteins, which are known to express LDN sequences.
      The anti-LDN in sera of infected mice were affinity purified on immobilized
      bovine milk glycoproteins and shown to specifically bind LDN. An IgM monoclonal
      antibody (SMLDN1.1) was derived from the spleens of S. mansoni infected mice and 
      shown to specifically bind LDN determinants. Immunoblots with affinity purified
      anti-LDN and SMLDN1.1 demonstrate that LDN sequences occur primarily on N-glycans
      of numerous glycoproteins of adult S. mansoni. LDN sequences are also expressed
      in many glycoproteins from S. japonicum and S. haematobium. The availability of
      antibody to LDN determinants should aid in defining the roles of these glycans in
      helminth and vertebrate biology.
FAU - Nyame, A K
AU  - Nyame AK
AD  - Department of Biochemistry and Molecular Biology, University of Oklahoma Health
      Sciences Center 73104, USA.
FAU - Leppanen, A M
AU  - Leppanen AM
FAU - DeBose-Boyd, R
AU  - DeBose-Boyd R
FAU - Cummings, R D
AU  - Cummings RD
LA  - eng
GR  - N01-AI-55270/AI/NIAID NIH HHS/United States
GR  - R01 AI42272-01/AI/NIAID NIH HHS/United States
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - England
TA  - Glycobiology
JT  - Glycobiology
JID - 9104124
RN  - 0 (Antibodies, Helminth)
RN  - 0 (Antibodies, Monoclonal)
RN  - 0 (Antigens, Helminth)
RN  - 0 (Disaccharides)
RN  - 0 (Glycoproteins)
RN  - 0 (Helminth Proteins)
RN  - 0 (Immunoglobulin M)
RN  - 0 (Oligosaccharides)
RN  - 0 (Polysaccharides)
RN  - 136198-41-9 (N-acetylgalactosaminyl-1-4-N-acetylglucosamine)
RN  - J2B2A4N98G (Lactose)
SB  - IM
MH  - Animals
MH  - Antibodies, Helminth/*blood
MH  - Antibodies, Monoclonal
MH  - Antigens, Helminth/chemistry
MH  - Carbohydrate Sequence
MH  - Cattle
MH  - Disaccharides/*immunology
MH  - Glycoproteins/chemistry/immunology
MH  - Helminth Proteins/chemistry/immunology
MH  - Immunoglobulin M/blood
MH  - Lactose/*analogs & derivatives/immunology
MH  - Mice
MH  - Molecular Sequence Data
MH  - Oligosaccharides/chemistry/immunology
MH  - Polysaccharides/chemistry/immunology
MH  - Schistosoma mansoni/*immunology
MH  - Schistosomiasis mansoni/*immunology
EDAT- 1999/10/16 00:00
MHDA- 1999/10/16 00:01
CRDT- 1999/10/16 00:00
PHST- 1999/10/16 00:00 [pubmed]
PHST- 1999/10/16 00:01 [medline]
PHST- 1999/10/16 00:00 [entrez]
AID - cwc109 [pii]
AID - 10.1093/glycob/9.10.1029 [doi]
PST - ppublish
SO  - Glycobiology. 1999 Oct;9(10):1029-35. doi: 10.1093/glycob/9.10.1029.