PMID- 10521512
OWN - NLM
STAT- MEDLINE
DCOM- 19991123
LR  - 20190508
IS  - 0021-9258 (Print)
IS  - 0021-9258 (Linking)
VI  - 274
IP  - 43
DP  - 1999 Oct 22
TI  - Regulation of bad phosphorylation and association with Bcl-x(L) by the MAPK/Erk
      kinase.
PG  - 31108-13
AB  - Phosphorylation of the Bcl-2 family protein Bad may represent an important bridge
      between survival signaling by growth factor receptors and the prevention of
      apoptosis. Bad phosphorylation was examined following cytokine stimulation, which
      revealed phosphorylation on a critical residue, serine 112, in a MEK-dependent
      manner. Furthermore, Bad phosphorylation also increased on several sites distinct
      from serine 112 but could not be detected on serine 136, previously thought to be
      a protein kinase B/Akt-targeted residue. Serine 112 phosphorylation was shown to 
      be absolutely required for dissociation of Bad from Bcl-x(L). These results
      demonstrate for the first time in mammalian cells the involvement of the Ras-MAPK
      pathway in the phosphorylation of Bad and the regulation of its function.
FAU - Scheid, M P
AU  - Scheid MP
AD  - Jack Bell Research Centre, University of British Columbia, Vancouver, British
      Columbia V6H 3Z6, Canada.
FAU - Schubert, K M
AU  - Schubert KM
FAU - Duronio, V
AU  - Duronio V
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - J Biol Chem
JT  - The Journal of biological chemistry
JID - 2985121R
RN  - 0 (BAD protein, human)
RN  - 0 (BCL2L1 protein, human)
RN  - 0 (Carrier Proteins)
RN  - 0 (Enzyme Inhibitors)
RN  - 0 (Flavonoids)
RN  - 0 (Phosphopeptides)
RN  - 0 (Proto-Oncogene Proteins)
RN  - 0 (Proto-Oncogene Proteins c-bcl-2)
RN  - 0 (bcl-Associated Death Protein)
RN  - 0 (bcl-X Protein)
RN  - 17885-08-4 (Phosphoserine)
RN  - 452VLY9402 (Serine)
RN  - EC 2.7.1.- (MAP2K2 protein, human)
RN  - EC 2.7.10.1 (Protein-Tyrosine Kinases)
RN  - EC 2.7.11.1 (AKT1 protein, human)
RN  - EC 2.7.11.1 (Protein-Serine-Threonine Kinases)
RN  - EC 2.7.11.1 (Proto-Oncogene Proteins c-akt)
RN  - EC 2.7.11.24 (Mitogen-Activated Protein Kinases)
RN  - EC 2.7.12.2 (MAP Kinase Kinase 1)
RN  - EC 2.7.12.2 (MAP Kinase Kinase 2)
RN  - EC 2.7.12.2 (MAP2K1 protein, human)
RN  - EC 2.7.12.2 (Mitogen-Activated Protein Kinase Kinases)
RN  - SJE1IO5E3I (2-(2-amino-3-methoxyphenyl)-4H-1-benzopyran-4-one)
SB  - IM
MH  - Apoptosis
MH  - Carrier Proteins/*metabolism
MH  - Cell Death
MH  - Cell Line
MH  - Enzyme Inhibitors/pharmacology
MH  - Flavonoids/pharmacology
MH  - Homeostasis
MH  - Humans
MH  - MAP Kinase Kinase 1
MH  - MAP Kinase Kinase 2
MH  - Mitogen-Activated Protein Kinase Kinases/metabolism
MH  - Mitogen-Activated Protein Kinases/*metabolism
MH  - Peptide Mapping
MH  - Phosphopeptides/chemistry
MH  - Phosphorylation
MH  - Phosphoserine
MH  - *Protein-Serine-Threonine Kinases
MH  - Protein-Tyrosine Kinases/metabolism
MH  - Proto-Oncogene Proteins/metabolism
MH  - Proto-Oncogene Proteins c-akt
MH  - Proto-Oncogene Proteins c-bcl-2/*metabolism
MH  - Serine
MH  - Substrate Specificity
MH  - bcl-Associated Death Protein
MH  - bcl-X Protein
EDAT- 1999/10/16 00:00
MHDA- 1999/10/16 00:01
CRDT- 1999/10/16 00:00
PHST- 1999/10/16 00:00 [pubmed]
PHST- 1999/10/16 00:01 [medline]
PHST- 1999/10/16 00:00 [entrez]
AID - 10.1074/jbc.274.43.31108 [doi]
PST - ppublish
SO  - J Biol Chem. 1999 Oct 22;274(43):31108-13. doi: 10.1074/jbc.274.43.31108.